Labshake search
Citations for Leica :
801 - 850 of 1438 citations for R 4 Benzyl 2 2 diphenylphosphino benzyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections were cut frozen on a sliding microtome (Leica SM2010 R) to a thickness of 40 μm ...
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bioRxiv - Neuroscience 2021Quote: ... Chromogenic detection was performed with the Bond Intense R Detection System (Leica, DS9263) or the DCS Supervision 2 Polymer System (Innovative Diagnostik-Systeme ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sectioned by a cryostat at −20°C (Leica, Germany, SM2010 R), and 30 μm coronal slices were made ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections of 30 μm thickness were obtained in microtome (Leica SM2010 R).
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Cell Biology 2024Quote: ... Resin blocks were trimmed to approximately 2 mm2 and sectioned at 70 nm using a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc., Buffalo Grove, IL) and a Diatome diamond knife (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was re-sectioned at 60 μm using a freezing microtome (Leica, SM2010 R). Next ...
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bioRxiv - Neuroscience 2021Quote: ... the brains were sliced at 50 microns using the freezing microtome (Leica, SM2010 R). Selected slices (1 in 5 serially ...
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bioRxiv - Cancer Biology 2024Quote: ... before endogenous peroxidase blocking using a BOND Intense R Detection kit (#DS9263, Leica Biomarkers) for 5 min ...
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bioRxiv - Physiology 2024Quote: ... Ultrathin sections (∼50 nm) were cut using a Leica Ultracut R microtome (Leica Microsystem) with a Diatome diamond knife (Diatome Ltd. ...
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bioRxiv - Neuroscience 2024Quote: ... Forty µm coronal sections were collected on a freezing microtome (Leica SM 2000 R) and stored in 1xPBS with 0.01% sodium azide at 4°C until processing for histological assessment.
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bioRxiv - Cell Biology 2024Quote: ... In vivo retinal thickness was measured using the Envisu-R SD-OCT imager (Leica Microsystems Inc. ...
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bioRxiv - Pathology 2024Quote: ... immersed in xylene and then transferred to molten Formula R paraffin wax (Leica, Germany). All viscera were processed using protocol 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... and Stellaris 5 (Leica Microsystems) confocal microscopes.
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal slices (30-50 μm thick) were made using a freezing microtome (Leica, SM2010 R). Staining was performed on free floating slices ...
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bioRxiv - Plant Biology 2022Quote: ... R-GECO1 was imaged using a Leica DMi8 inverted microscope (Leica Microsystems, Milton Keynes, UK) with a 63x 1.4NA oil immersion objective ...
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bioRxiv - Physiology 2021Quote: ... Ultrathin sections (~40 nm) were cut in a Leica Ultracut R microtome (Leica Microsystem, Austria) using a Diatome diamond knife (DiatomeLtd ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μm tissue sections were prepared using a Leica SM2010 R Sliding Microtome (Leica Biosystems). Slides were heated at 60°C for 20 min ...