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Citations for Leica :
801 - 850 of 997 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Four to five random fields per cell line and coverslip were imaged using the Leica SP8 confocal microscope (Leica Camera) or the Zeiss Apoptome 2 (Zeiss) ...
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bioRxiv - Pathology 2022Quote: ... with RNAscope 2.5 LS Reagent Kit-Red (322150, Advanced Cell Diagnostics, Newark, CA) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse Ppib to confirm adequate RNA preservation and detection ...
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bioRxiv - Cell Biology 2022Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 100x objective using a high-resolution light microscope (Leica DM6000 BD ...
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bioRxiv - Genetics 2023Quote: ... AID::GFP levels were measured by imaging fluorescence in body wall muscle cells in the head of living animals on a Leica TCS SP8 microscope (LAS AF software, Leica). AID::GFP expression levels were quantified in Fiji ImageJ.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Molecular Biology 2023Quote: Exponentially growing cells were labelled with 25mM IdU and 125mM CldU (as indicated, followed by cell lysis, fiber spreading with staining carried out as previously described2. Images were obtained using a Leica SP8 confocal microscope using a 63x oil objective ...
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bioRxiv - Biophysics 2023Quote: ... The collagen gel is stretched uniaxially in 1% step increments using a custom made stepper motor-driven cell stretching device (46) mounted on an epifluorescence microscope (Leica DMI6000 CS equipped with a 20x Leica HCX PL Fluotator objective ...
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bioRxiv - Biophysics 2023Quote: ... The collagen gel is stretched uniaxially in 1% step increments using a custom made stepper motor-driven cell stretching device (46) mounted on an epifluorescence microscope (Leica DMI6000 CS equipped with a 20x Leica HCX PL Fluotator objective ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Immunology 2023Quote: ... Cells were imaged directly using the 40x objective on a SP8X WLL (white light laser) microscope (Leica Microsystems, Wetzlar, Germany) with climate control at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx.) were injected in the duct of Cuvier under a M205 FA Fluorescence stereomicroscope (Leica), as previously described (Stoletov et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Ratiometric FRET imaging of living cells expressing talin tension sensors was performed using an epi-fluorescence microscope (Leica DMI 8000) with excitation at 496-516 nm from Leica LED8 system ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Developmental Biology 2023Quote: ... organoids were cryopreserved, embedded in OCT (Cell Path Ltd, Newtown, UK) and cut (10 µm) on a cryostat (Leica Cm1860). IF was performed as previously described [59] ...
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bioRxiv - Cancer Biology 2023Quote: ... with RNAscope 2.5 LS Assay Reagent Kit-Red (322150, Advanced Cell Diagnostics) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse peptidylprolyl isomerase B ...
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2023Quote: ... were imaged at different intervals to determine cell health and viability using a Leica DM IL microscope (Leica Microsystems, Switzerland).
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bioRxiv - Cell Biology 2023Quote: ... and 18.4 nL of a cell suspension at 108 cells/mL were injected in the duct of Cuvier of the embryos under a M205 FA stereomicroscope (Leica). Injected embryos were maintained at 28°C in between injection and imaging ...
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bioRxiv - Cell Biology 2023Quote: ... The last 3 washes were then performed and cells were imaged directly at the 96-well plate with an inverted DMi8 microscope (Leica). For image acquisition the same exposure times were used for each correspondent channel ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing OMM-Cepia3 and ER-mit RspA-splitFAST were mounted into an open-topped chamber in mKRB supplemented with TFCoral (5 µM) and imaged by a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
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bioRxiv - Cell Biology 2023Quote: Imaging of the different splitFAST-based probes for MCSs by fluorescence microscopy was performed on a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
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bioRxiv - Neuroscience 2024Quote: ... Transverse hippocampal slices (300 µm for whole-cell recording while 400 µm for fEPSP recording) were cut in the cutting solution using a vibratome (VT-1200S, Leica) and transferred to artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Cancer Biology 2024Quote: ... the SignalStar™ Multiplex Immunohistochemistry Assay (Cell Signalling Technologies) for Use on BOND RX Fully Automated Research Stainer (Leica Biosystems) was followed as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Fos-expressing cells were counted at 5x magnification bilaterally using a computer-assisted image analysis system (LAS AF 2D, Leica). A semi-transparent spinal cord atlas image (Supplementary Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Acute sagittal slices (250 um) of the cerebellum containing intact Purkinje cells were obtained using a vibrating tissue slicer (VT1200S; Leica) and incubated in ACSF (125 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... 13.8nL of cell suspension at 100.106 cells per ml were injected in the duct of Cuvier of the embryos under the M205 FA stereomicroscope (Leica). Polyacrylamide microgel beads at a respective stock concentration of 1.6kPa-Alexa Fluor 633 at 83 x 106 beads/ml) ...
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bioRxiv - Cancer Biology 2024Quote: The earliest bead/cell circulation and arrest events following injection in the zebrafish embryo were captured with the M205 FA stereomicroscope (Leica) under which the injections were performed ...
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bioRxiv - Cell Biology 2020Quote: ... We then pelleted the yeast cells by filtering and carried out high pressure freezing with the Leica ICE system (Leica Biosystems). We further processed the frozen cell pellets by freeze substitution (FS ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was for 40 h at – 20°C followed by 40 h at +20°C Ultrathin-sections of Lowicryl-embedded material were collected on Formvar-carbon-coated gold grids (200 mesh ...
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bioRxiv - Evolutionary Biology 2021Quote: ... differentiation of mother and daughter cells was possible and intracellular replication was observed by fluorescence microscopy (Leica DM5500B and Leica DFC360).
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bioRxiv - Evolutionary Biology 2021Quote: ... differentiation of mother and daughter cells was possible and intracellular replication was observed by fluorescence microscopy (Leica DM5500B and Leica DFC360).
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bioRxiv - Neuroscience 2021Quote: ... A block of tissue containing the hippocampus was prepared and transverse hippocampal slices (300 and 400 µm thickness for whole-cell and field recordings, respectively) were cut with a vibratome (Leica VT1000S). Slices were transferred to artificial CSF (ACSF ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was mounted with Vectashield mounting media with DAPI and viewed for colocalization of virally labeled and immunolabeled cell bodies using epifluorescence microscopy (Leica DM2500). Additional sections from an additional animal (without immunohistochemistry ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were mounted using mounting media and imaged under 63X oil immersion objective of the SP8 confocal microscope (Leica, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were mounted using mounting media and imaged under 63X oil immersion objective of the SP8 confocal microscope (Leica, Germany).
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bioRxiv - Cell Biology 2021Quote: Localisation of each fusion protein in transfected NSC-34 cells was characterised by imaging using a TCS SP5 II confocal microscope with a 63× oil-immersion objective lens (Leica Microsystems). Imaging was carried out 48 h post-transfection.
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bioRxiv - Systems Biology 2020Quote: ... ‘Force- loaded’ images (with cells) of the beads embedded in the polyacrylamide gels were obtained using a 40X oil-immersion objective (Leica Microsystems). The ‘null-force’ image was obtained at the end of each experiment by adding trypsin to the cells for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut into 300μm thick (for whole-cell experiments) or 500μm thick (for field recordings) coronal sections using a vibratome (VT1000S, Leica, Nussloch, Germany). Slices were transferred to an incubation chamber filled with warmed ACSF containing (in mM) ...
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bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
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bioRxiv - Cancer Biology 2020Quote: The lymphocyte cells expressing CD4+ and CD8+ were counted by a pathologist using Aperio Image Toolbox analysis software (Aperio, Leica Biosystems) and expressed as cell density (CD4+ and CD8+ cells/mm2 of analyzed tissue ...
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bioRxiv - Cell Biology 2020Quote: Live images of cells under stress and FRAP experiments (described below) were acquired using a Leica DMi8 STP800 (Leica, Bannockburn, IL) equipped with a X-light V2 Confocal Unit spinning disk spinning disk unit ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.
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bioRxiv - Biophysics 2020Quote: ... ‘Force-loaded’ images (with cells) of the beads embedded in the polyacrylamide gels were obtained using a 60× oil-immersion objective (Leica Microsystems). The ‘null-force’ image was obtained at the end of each experiment by adding trypsin to the cells for 1 h ...
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bioRxiv - Bioengineering 2022Quote: The imaging experimental setup consisted of Leica LASX live cell imaging workstation on DMi8 inverted fluorescence microscope (Leica Microsystems Wetzlar, Germany). An Andor iXON 897 Ultra back integrated camera was used for imaging ...
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bioRxiv - Bioengineering 2022Quote: The liver with repopulated bile ducts of GFP-expressing cells was cultured in the circulation culture system for 5 days and imaged using confocal microscopy (Leica Microsystems). A reconstructed 3D image was prepared from Z-stack images using ImageJ (National Institutes of Health).
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...