Labshake search
Citations for Leica :
801 - 850 of 1003 citations for IL 33 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... brains were submerged in ice-cold and oxygenated media (DMEM High-Glucose without phenol red, GibcoTM, USA) and sectioned in a Leica VT100S vibratome (Leica, Buffalo Grove, IL) set to 300 μm in the z-direction ...
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bioRxiv - Cell Biology 2022Quote: ... Whole wells were supplemented with 50 μl of PBS post staining and well were scanned on the DMi8 fluorescent microscope (Leica, Buffalo Grove, IL)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Digital images of these sections were captured with 10-100 × objectives using upright fluorescent microscopy (Leica DM6, Leica Microsystems Inc., Buffalo Grove, IL). Analyses were performed by investigators blinded to the sample identification.
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Pathology 2022Quote: ... in situ hybridization was performed on 12 µm-thick sections of 4% PFA-fixed OCT mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval (HIER ...
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bioRxiv - Genetics 2022Quote: ... Light was delivered in pulses of 30s of blue LED (from the LED light source of the inverted laboratory microscope LEICA DM IL LED) at 1 Hz (Table S3) ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Pathology 2021Quote: ... was performed on 5µm-thick sections of formalin-fixed lung mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Plant Biology 2021Quote: ... Embedments were cut into small blocks and mounted in the vise-chuck of a Reichert Ultracut UCT ultramicrotome (Leica, Buffalo Grove, IL, USA). Ultrathin sections (∼60 to 70 nm ...
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bioRxiv - Plant Biology 2021Quote: ... GFP and YFP signals were imaged on a Leica SP2 or Leica Stellaris 8 Laser Scanning Confocal Microscope (Leica Microsystems, Buffalo Grove, IL) at 48hpi ...
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bioRxiv - Neuroscience 2021Quote: ... brightfield images of DAB-stained brain sections were acquired at 40x using an Aperio AT2 slide scanner (Leica Biosystems Inc., Buffalo Grove, IL). Digital images were subsequently exported from Aperio Imagescope (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... leaving the left and right hemispheres intact and coronal cortical slices were cut to a thickness of 300μm using a vibratome (Leica VT1200, Buffalo Grove, IL). After cutting ...
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bioRxiv - Biochemistry 2019Quote: ... cells were transferred to a 12 mm glass base dish and imaged with a Leica TCS SP8 confocal imaging system (Leica Microsystems Inc, IL) using an HC PL APO 40x/1.3 oil CS2 objective ...
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bioRxiv - Cell Biology 2019Quote: ... Tissue was then treated with 70% ethanol prior to paraffin embedding using a Leica eASP300 enclosed tissue processor (Leica Biosystems, Buffalo Grove, IL). Paraffin processed tissues were cut into 5 micron section and slide mounted using a HM 355S Automatic Microtome (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... All sections were imaged and analyzed at 20x optical zoom using an Aperio CS2 digital slide scanner (Leica Biosystems Inc., Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: ... stain quantification of total tumor area (necrotic and stromal areas removed) and percent positive stain or stain intensity was performed using ImageScope Aperio Analysis software (Leica, Buffalo Grove, IL). Areas for quantification were annotated using Aperio analysis tools ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... Hydrogels were imaged using DMi8 Yokogawa W1 spinning disk confocal microscope with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL) by taking z-stacks at 10x magnification with a step size of 5 µm and a total depth of 200 µm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of paraffin sections with 8 µm thickness were continuously cut using a Leica RM2255 microtome (Leica Biosystems Inc. Buffalo Grove, IL) and mounted on Superfrost Plus microscope slides (cat# 12-550-15 ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0 mm deep with a controlled velocity of 5.0+0.2 m/s and a dwell time of 100 ms using a stereotaxic electromagnetic impactor (Impact one, Leica; Buffalo Grove, IL, USA) equipped with a 5.0 mm flat steel tip ...
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Myofiber injury induces capillary disruption and regeneration of disorganized microvascular networksbioRxiv - Physiology 2021Quote: ... or 25X water immersion objective (NA = 0.95; image size, 465 µm x 465 µm) on a laser scanning confocal microscope (TCS SP8, Leica Microsystems; Buffalo Grove, IL) using Leica LASX software ...
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bioRxiv - Neuroscience 2021Quote: ... Sagittal vermal slices and coronal floccular slices at 50μm were obtained from two animals using Leica VT1000S vibratome (Leica Biosystems, Buffalo Groves, IL). For immunolabeling of inhibitory synapses and the unipolar brush cells ...
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bioRxiv - Pathology 2021Quote: ... The RNAscope® ISH assay was performed using the RNAscope 2.5 LSx Reagent Kit (Advanced Cell Diagnostics, Newark, CA) on the automated BOND RXm platform (Leica Biosystems, Buffalo Grove, IL) as described previously (23) ...
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bioRxiv - Biophysics 2022Quote: ... Embedded brains were then immersed in ice-cold and oxygenated DMEM media without phenol red and then sectioned in a Leica VT100S vibratome (Leica, Buffalo Grove, IL). 300 mm thick brain tumor sections were transferred to laminin coated cell culture insert (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: Cell pellets in cryoprotectant solution were loaded onto membrane carriers (Mager Scientific, Dexter, MI) and cryo-fixed using the EM PACT2 high pressure freezer (Leica, Buffalo Grove, IL). Cryo-fixed cells were processed by freeze substitution using an AFS2 automated freeze substitution unit (Leica) ...
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bioRxiv - Molecular Biology 2022Quote: Wound healing response at day 7 and scar tissue morphology and ECM architecture at day 30 post-wounding were determined by hematoxylin and eosin (H&E) staining and Masson’s Trichrome Staining respectively (Leica Biosystems, Buffalo Grove, IL). Epithelial gap ...
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bioRxiv - Physiology 2022Quote: ... All samples were imaged using a Leica SP8 confocal/multiphoton microscope and Coherent Chameleon Vision II Ti:Sapphire laser (Leica, Buffalo Grove, IL, USA) tuned to a wavelength of 910 nm and the Leica application suite (LAX ...
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bioRxiv - Physiology 2022Quote: ... Images were acquired with a HC PL APO 10/0.40 CS2 objective on an inverted laser scanning confocal microscope (TCS SP8) using LASX software (all from, Leica Microsystems; Buffalo Grove, IL). A Dragonfly High Speed Confocal Microscope System [Leica DMi8 microscope coupled to an Andor Dragonfly 200 and integrated Fusion software (Oxford Instruments ...
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bioRxiv - Microbiology 2022Quote: ... Tumor multiplicity was determined by a double- blind gross counting of colonic tumors visualized with a Leica M165FC microscope (Leica, Buffalo Grove, IL) at 5X magnification (Amos-Landgraf et al. ...
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bioRxiv - Plant Biology 2022Quote: Images were acquired from different infiltrated leaf sections of tobacco at 12-72 h after leaf inoculation using a Leica TCS SP5 laser scanning confocal microscope (Leica Microsystems, Bannockburn, IL). Images were acquired in the XYZ plane in 0.25 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Sections of 65 nm were cut from the outer surface of the tissue with an ultramicrotome UC7 (Leica Microsystems Inc., Buffalo Grove, IL) using a diamond knife (Diatome ...
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bioRxiv - Neuroscience 2023Quote: ... and the medial geniculate nucleus (MGN) was isolated and 500 μm thick thalamocortical slices were cut using a vibrating microtome (Leica, Deer Parl, IL) in ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2022Quote: ... Digital images of these sections were captured with 10-100 × objectives using upright fluorescent microscopy (Leica DM6, Leica Microsystems Inc., Buffalo Grove, IL). Analyses were performed by investigators blinded to the sample identification ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The morphologies of the spheroids were captured at various time points using an inverted light microscope equipped with a digital camera (Leica DM-IL MC170).
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then frozen rapidly on dry ice before being coronally sectioned into 100 μm sections using a Leica CM3050 cryostat (Leica Biosystems, Richmond, IL). Slices were placed in 0.01M PBS and the ones containing the site of infection were selected to be stained ...
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bioRxiv - Neuroscience 2023Quote: ... A stainless-steel circular impactor tip (5.0 mm diameter) was positioned over the craniectomy and an electromagnetic controlled cortical impactor (Leica Biosystems, Buffalo Grove, IL) was used to induce a focal injury (2.5 mm depth ...
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bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
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bioRxiv - Cell Biology 2023Quote: ... Bright-field images were acquired using a Leica Leitz DMRXE upright microscope equipped with a DCF 300FXRI camera and Leica FireCam software (Leica Microsystems, Bannockburn, IL). Fluorescent images were acquired using a Leica SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Pathology 2023Quote: ... the vibratome stage with attached tissue was inserted into the vibratome (Model G Oxford Vibratome or Leica VT1200S, Leica Biosystems, Deerfield, IL, USA), surrounded by fresh ice ...
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bioRxiv - Microbiology 2023Quote: ... The size of the aggregates was calculated from images of live aggregated Spn captured using a Leica LMD6 with DFC3000G-1.3-megapixel monochrome camera at 40X magnification (Leica Biosystems, Buffalo Grove, IL). The top five largest aggregates were determined using ImageJ (NIH ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining images were taken using a Leica inverted microscope with a TCS SPEII confocal module and processed using LAS X software (Leica Microsystems Inc, IL).
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bioRxiv - Cell Biology 2023Quote: ... Acquisition and quantification of cell migration was performed using the motorized stage of an inverted video microscope (Leica DMIRB, Leica Microsystems, Deerfield, IL) and the cell counter plugin of the ImageJ software as described previously (Van Goethem et al. ...
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bioRxiv - Microbiology 2023Quote: ... were captured for each sample using a Leica LMD6 with DFC3000G-1.3-megapixel monochrome camera at 10X magnification (Leica Biosystems, Buffalo Grove, IL). Size of live bacterial aggregates was based on the top ten largest aggregates obtained using ImageJ (NIH ...
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bioRxiv - Cancer Biology 2024Quote: ... was performed on sections of formalin-fixed paraffin-embedded organoids (FFPE) using a Bond automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA) by the Translational Research Unit (TRU ...