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Citations for Leica :
801 - 850 of 1203 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: Immunohistochemistry (IHC) was performed on kidney sections using an automated stainer and system reagents (Leica Bond RX, Leica Biosystems, Buffalo Grove, IL). IHC stains were selected based on their routine use in published studies of the adenine diet model and included the F4/80 stain for murine macrophages and the KIM-1 and NGAL stain for renal tubular injury9 ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples were then cut into 12-micron sections for immunofluorescence staining using a Leica CM1520 cryostat (Leica Biosystems, Deer Park, IL). Samples were first fixed in methanol in −20°C then treated with Antigen Retrieval Buffer (pH 6 ...
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bioRxiv - Cell Biology 2023Quote: ... USA) and Bioptigen Envisu R2200 ultra-high resolution (UHR) spectral-domain optical coherence tomography system (SD-OCT; Leica Microsystems, Deerfield, IL, USA) (22) ...
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bioRxiv - Neuroscience 2023Quote: Each NDG was sectioned at 16 μm into serial sections using a Leica CM3050 S cryostat (Leica, Buffalo Grove, IL, United States). Sections were immediately mounted and slides were dipped in ethanol (100% ...
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bioRxiv - Genomics 2022Quote: ... The crickets where then observed under a fluorescent dissecting microscope with digital camera attached (Leica M125, Leica Microsystems Inc., Deerfield, IL, USA) for either different eye coloration from wild type in knock-out treatments (using a standard white LED ring light ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... then sectioned into 250 μm-thick slices in the oxygenated ice-cold cutting solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1200S; Leica, Deerfield, IL). The slices collected were allowed to recover at 30°C for 20 minutes in oxygenated saline solution (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was removed from the skull and sliced at 250 μm using a vibrating microtome (VT1000s, Leica Biosystems, Buffalo Grove, IL) in cold ...
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bioRxiv - Immunology 2023Quote: ... The coverslips were then mounted on glass slides and imaged using a confocal microscope (LEICA TCS SP8, Leica Microsystems, Buffalo Grove, IL) with a 40 X oil objective.
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bioRxiv - Neuroscience 2024Quote: ... Selected sections were also studied on a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) or scanned with an Olympus VS200 Research Slide Scanner (Olympus / Evident ...
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bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
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bioRxiv - Neuroscience 2024Quote: Hemibrains were drop-fixed in 4% paraformaldehyde for two days and sectioned at 40 mm on a vibratome (Leica, Buffalo Grove, IL). Sections were collected in a 1 in 4 series and stored in PBS at 4°C ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-CD4 primary antibody (clone 4B12, Leica Biosystems ...
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bioRxiv - Genomics 2022Quote: ... mouse brains were transferred to cryostat (Leica CM1950) and cut into 20 µm thick slices in coronal sections at -20 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-GFAP (PA0026, Leica Biosystems, Wetzler, Germany), or mouse anti-Factor VIII (ab41187 ...
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bioRxiv - Neuroscience 2022Quote: ... GFAP (anti-GFAP (mouse, GA5, Leica, 1:200) and IBA1 (1:1000 Wako ...
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bioRxiv - Pathology 2024Quote: ... CD163 (mouse monoclonal antibody, clone 10D6; Leica Biosystems), and CD34 (mouse monoclonal antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... PowerVision Poly-HRP anti-Mouse IgG (Leica, PV6119) as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse-anti-TRP53 (CM5, Novocastra™ Leica Biosystems); mouse-anti-p19/ARF (5.C3.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... was done on sections of FFPE tissue using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA), with the following antibodies and conditions (dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Acquisition and quantification of cell migration was performed using the motorized stage of an inverted video microscope (Leica DMIRB, Leica Microsystems, Deerfield, IL), the Metamorph software and the cell counter plugin of the ImageJ software as described (Van Goethem et al. ...
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bioRxiv - Cell Biology 2019Quote: Microscope images of fixed Drosophila follicles were obtained using LAS AF SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20×/0.60 IMM CORR -/D (Leica Microsystems, Buffalo Grove, IL), Zen software on a Zeiss 880 mounted on Zeiss Axio Observer.Z1 using Plan-Apochromat 20x/0.8 working distance (WD ...
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bioRxiv - Immunology 2022Quote: ... was performed on sections (5 mm thick) of formalin-fixed lung mounted onto charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... The nuclei were imaged using a Leica DM6 B microscope equipped with Leica DMC5400 camera or DM IL LED fluorescence microscope equipped with Leica DFC450 C (Leica Microsystems, Wetzlar, Germany) camera and the images were processed using ImageJ software version 1.53 (National Institutes of Health ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Cell Biology 2022Quote: ... cryosectioned parallel (axially) to the optic axis at 20 μm thickness using a Leica CM3050 S cryostat (Leica Biosystems Inc; Buffalo Grove, IL), and finally were transferred onto plain microscope slides ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The fixed organs were moved to a 70% ethanol solution and processed with an ASP300 S tissue processor (Leica Biosystems, Buffalo Grove, IL) for dehydration into paraffin ...
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bioRxiv - Physiology 2019Quote: Fixed whole EHBDs were imaged using a Leica SP8 confocal/multiphoton microscope and Coherent Chameleon Vision II Ti:Sapphire laser (Leica, Buffalo Grove, IL, USA) tuned to a wavelength of 910 nm ...
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Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivobioRxiv - Cell Biology 2019Quote: Microscope images of fixed Drosophila follicles were obtained using LAS AS SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20x/0.60 IMM CORR -/D objective (Leica Microsystems, Buffalo Grove, IL) or using Zen software on a Zeiss 700 LSM mounted on an Axio Observer.Z1 using a Plan-Apochromat 20x/0.8 working distance (WD ...
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bioRxiv - Cancer Biology 2020Quote: ... stain quantification of total tumor area (necrotic and stromal areas removed) and percent positive stain or stain intensity was performed using ImageScope Aperio Analysis software (Leica, Buffalo Grove, IL). Areas for quantification were annotated using Aperio analysis tools and percent weak ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were then rinsed briefly with PBS and then sectioned at a thickness of 100 μm on a vibratome (VT1200 Leica Biosystems, IL, USA). Brain sections were incubated in a blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... Each slide was analyzed by an investigator blinded to the identity of the experimental material using a Leica SP8 upright confocal microscopy (Leica Microsystems, IL, USA) and ImageJ and Plugin Cell Counter (NIH Image ...
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Sterol flow between the plasma membrane and the endosome is regulated by the LAM family protein Ltc1bioRxiv - Cell Biology 2019Quote: Filipin and Calcofluor imaging (Fig1G, 2D, 6B) was done by wide field microscopy acquired with the Leica AS AF software (Leica MicroSystems, Bannockburn, IL).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Cell Biology 2021Quote: Microscope images of fixed Drosophila follicles were obtained using LAS AS SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20x/0.60 IMM CORR -/D objective (Leica Microsystems, Buffalo Grove, IL) or using Zen software on a Zeiss 700 LSM mounted on an Axio Observer.Z1 using a Plan-Apochromat 20x/0.8 working distance (WD ...
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bioRxiv - Neuroscience 2021Quote: Slices were transferred to the recording chamber mounted on the fixed stage of a Leica DM6000 FS microscope (Leica Miscrosystems Inc. Bannackburn, IL) and perfused at ≈2mL/min with ACSF maintained at 32°C ...
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bioRxiv - Biochemistry 2020Quote: ... Imaging of mitotic cells was carried out with a Leica DMI6000 microscope (Leica DFC360 FX Camera, 63x/1.40-0.60 NA oil objective, Leica AF6000 software, Buffalo Grove, IL) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 40pm horizontal sections of the thalamus (D/V depth −5.3 mm to 6.0 mm) were obtained using a Leica VT 1000S microtome (Leica Biostystems, Buffalo Grove, IL, USA) and collected in 0.1 M phosphate buffer (PB ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... and parasagittal slices containing the vermis of the cerebellum were cut at 350 mm on a Leica VT1200 (Leica Microsystems, Buffalo Grove, IL). Sections were incubated in a holding chamber maintained at 32°C ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on sections of formalin-fixed paraffin-embedded patient tissue (FFPE) using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA). Slides were de-paraffinized and heat-mediated antigen retrieval using the Bond Epitope Retrieval 1 solution at pH6 (H1 ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL). For identification of DAT-specific deletion of PERK and consequential reduction of phosphorylated eIF2α (p-eIF2α ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Immunology 2021Quote: ... Additional sections were cut for immunohistochemistry for both CD4 and F4/80 which were stained using the Leica Bond Rx automated immunostainer (Leica, Buffalo Grove, IL). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were processed and embedded in paraffin at the University of Michigan Microscopy & Image Analysis Core Facility using a Leica ASP 300 paraffin tissue processor/Tissue-Tek paraffin tissue embedding station (Leica, Buffalo Grove IL). Tumor explants were used for collagenase treatment ...