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Citations for Leica :
801 - 850 of 1298 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The brains were then embedded in OCT and preserved at –80°C until cryosectioning at 60 µm (Leica CM3050). For free-floating immunostaining ...
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bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
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bioRxiv - Microbiology 2023Quote: ... Trypanosomes at 1x106 parasites/mL were maintained in matrix-dependent HMI-11 medium containing 0,5% or 1.1% methylcellulose at 37°C and were observed under the 10x objective of an inverted DMI-4000B microscope (Leica) coupled to an ORCA-03G (Hamamatsu ...
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bioRxiv - Neuroscience 2023Quote: ... samples were transferred under liquid nitrogen to an automated freeze substitution system at -90 °C (EM AFS2, Leica Microsystems). Using pre-cooled tweezers ...
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bioRxiv - Cell Biology 2023Quote: ... the high-pressure-frozen samples were freeze-substituted in 0.2% glutaraldehyde with 0.2% uranyl acetate in acetone at -90°C in an automated freeze-substitution device (Leica AFS). After 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
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bioRxiv - Cell Biology 2023Quote: ... Blocks were equilibrated at -20°C for 1 h prior to cutting 12-µm sections using a cryostat (Leica Biosystems Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... equipped with Mai-Tai and Insight X3 tunable lasers (Spectral Physics) and a 37 °C preheated 40X objective (NA 1.10, HC PL IRAPO, Leica). The specimens were excited at 910 nm.
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bioRxiv - Microbiology 2023Quote: ... and inhibitor-induced effects on worm motility assessed using a stereo microscope at 10x magnification (M125 C, Leica, Germany) using the following scores ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence lifetime imaging microscopy (FLIM) analysis was performed at 37°C using a confocal laser scanning microscope (SP8, Leica) equipped with a Fast Lifetime Contrast (FALCON ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... Images and videos were acquired using an SP8 confocal microscope system with a 63x oil-corrected lens (C-Apochromat, numerical aperture, NA = 1.4) and Lightning deconvolution software (Leica), and processed using ImageJ version 2.9.0/1.53t (109) ...
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bioRxiv - Genetics 2024Quote: ... Sections were floated onto a 45 °C water bath and mounted on Leica “X-tra” adhesive slides (Leica biosytems), drained ...
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bioRxiv - Biophysics 2022Quote: GFP confocal line scanning microscopy was performed in a Leica TCS SP5 II CW-STED microscope using a 63x Oil Numerical Aperture 1.4 objective (Leica HC PL APO 63x/1.40 Oil CS), using a multiline Argon laser at 488 nm for excitation ...
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bioRxiv - Neuroscience 2019Quote: ... A Leica Refine-HRP kit (Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and stored at -80°C before sectioning on a CM1860 UV cryostat (Leica). 6μm-thick slides were stained with TUNEL (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by a final step of fresh paraffin incubation for another 20 min at 65°C (Tissue Infiltration Medium by Surgipath available through Leica). The paraffin embedded tissue was sectioned in Richard-Allan Type 9 paraffin (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Freshly-extracted brains were frozen in isopentane at –80°C and then cut into 10 μm sections with a CM1950 Cryostat (Leica). After cutting ...
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bioRxiv - Developmental Biology 2022Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded organoid at −20 °C using a cryostat (Leica). Cryosections were permeated with in PBST at temperature for 30 min and blocked with sheep serum (Zhongshanjinqiao ...
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bioRxiv - Immunology 2022Quote: ... target cells were labeled with CMTMR at 10 µM and 1:1000 Hoechst 33342 for 30 min at 37°C, and live imaged in ibidi 8 well chamber slides (ibiTreat, #80826) by confocal microscopy (SP8, Leica) with z-stacks (0.22 µm step ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Neuroscience 2019Quote: ... Imaging was performed for 5min at 1Hz at 37°C 5% CO2 on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40 ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Developmental Biology 2021Quote: ... rtTA cells were placed in PBS and imaged at 37°C using a TCS SP8 STED 3x microscope (Leica Microsystems) equipped with white-light laser ...
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bioRxiv - Neuroscience 2020Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Physiology 2021Quote: Serial OCT mounted cryostat sections (10 µm in thickness) were cut at -14°C (Leica Cryostat (Fisher Supply, Waltham, MA)) ...
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bioRxiv - Bioengineering 2021Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded lungs tissue at -20 °C using a cryostat (Leica). Cryosections were blocked with 5% normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2020Quote: ... equipped with a GFP filter (470 ± 40 nm excitation and 525 ± 50 nm emission) and documented with the attached camera (DFC425 C, Leica). Leaf discs were cut from positive fluorescent plants using a 7 mm cork borer and placed with the abaxial side up in the bottom of transparent Nunc® 96-wells plates (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Systems Biology 2021Quote: ... The samples were stored at −80°C until 10-15 μm coronal sections were cut using a cryostat (Leica CM3050). The sections were immediately placed on the functionalized coverslips described above ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of the IC (200 µm) were cut in ∼34 °C ACSF with a vibrating microtome (VT1200S, Leica Biosystems) and incubated at 34 °C for 30 min in ACSF bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue-Tek) at −20 °C and serial sagittal sections of 20 μm thickness were obtained using a cryostat (Leica, CM1860). Brain sections were pre-mounted on positively charged slides and washed in TBS ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Biophysics 2022Quote: ... Samples were cross-sectioned into slices of 10 μm thickness by cryo-microtomy at −15°C (CM3050 S, Leica, Austria). The transversal sections were put onto microscope slides with a drop of D2O ...
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bioRxiv - Cell Biology 2022Quote: ... Infiltrated tissues were then casted into molds together with liquid paraffin (65°C) and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate) ...
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bioRxiv - Microbiology 2020Quote: ... The samples were cryo-fixed by plunge freezing at −180°C in liquid ethane using a Leica EMGP (Leica, Austria). Grids were observed at 200kV with a Tecnai F20 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal sections of the IC (200 µm) were cut in 34°C ACSF with a vibrating microtome (VT1200S, Leica Biosystems) and incubated at 34°C for 30 min in ACSF bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μl of the solution was applied on glow discharged C-flatTM grids (Protochips) and the grids were blotted and frozen with KF80 specimen plunge-freezing device (Leica) or Vitrobot Mark IV (Thermo Fisher Scientific)
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bioRxiv - Plant Biology 2019Quote: ... were mounted on slides in ½ MS liquid media (0% sucrose, pH 5.7, 21°C) and imaged using a TCS-SP8 microscope (Leica, Germany) equipped with a 10X/0.3 NA or 63X/1.2 NA water immersion objective ...
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bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 33° C for the for at least 24 h before being imaged with a fluorescence on a microscope (Leica).