Labshake search
Citations for Leica :
801 - 850 of 1112 citations for 5 NITRO BENZO B THIOPHENE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: All experiments were imaged using a Leica SP-5 confocal microscope using a Plan-Apochromatic 63x 1.40 NA oil-immersion objective (Leica microsystems, 506210). Fluorophores were excited using a 100 mW Ar laser (458 ...
-
bioRxiv - Plant Biology 2019Quote: ... The root segments were embedded in agarose 5% and cut into 25 μm radial sections with a Leica VT1200S Leica vibratome (Leica Microsystems). Sections were categorized according to their distance (100 ...
-
bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
-
bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...
-
bioRxiv - Neuroscience 2022Quote: ... Their brains were quickly dissected out and stuck on a 5 mm agar stage before being mounted on a vibratome (Leica VT1200S) equipped with a sapphire blade ...
-
bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
-
bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
-
Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Confocal images were captured at 63X oil immersion objective on Leica STELLARIS 5 (Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X software ...
-
bioRxiv - Cell Biology 2023Quote: ... All confocal images were acquired using the Leica TCS SP8 or Stellaris 5 confocal microscope with the Leica LAS X software (Leica Microsystems). Following objectives were used with the Leica TCS SP8 ...
-
bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
-
bioRxiv - Cell Biology 2023Quote: ... Slices were imaged with a laser scanning confocal Stellaris 5 or TCS SP8 gSTED equipped with a white light laser and a 405-diode ultraviolet laser (Leica Microscope). Image acquisition was done using the LAS-X software in accordance with the Nyquist sampling sequential mode by exciting the fluorophores and collecting their signal with hybrid detectors (HyDs).
-
bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
-
bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
-
bioRxiv - Plant Biology 2023Quote: Lengths of root RAM and EZ regions and root hairs in 5-d-old seedlings grown on vertical MS medium without sucrose were measured under a Leica microscope (Leica DM5000). The data were analyzed using the ImageJ software (http://rsb.info ...
-
bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
-
bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
-
bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
-
bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
-
bioRxiv - Developmental Biology 2023Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
-
bioRxiv - Neuroscience 2024Quote: ... the lumbar spinal cord from L3-5 was mounted on an agarose mold and the dorsal halve was micro dissected on a vibratome (Leica Microsystems). Next ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
-
bioRxiv - Evolutionary Biology 2023Quote: ... LEGE 07298 and Phormidesmis priestleyi ULC 007 stained with either calcein or 5-CF were acquired with an inverted confocal laser scanning microscope (CLSM; Leica SP8) using a HC PL APO x63/1.40 oil objective ...
-
bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S ...
-
bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
-
bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
-
bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
-
bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
-
bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
-
bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
-
bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
-
bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
-
bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
-
CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
-
bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and imaged with a Leica TCS SP5 Confocal Laser Scanning Microscope (Leica) using a 40X water immersion objective.
-
bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
-
bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
-
bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
-
bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
-
bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...