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Citations for Leica :
801 - 850 of 983 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
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bioRxiv - Plant Biology 2024Quote: ... The cells were then washed 3 times for 5 min with PBS and covered with PBS before imaging them with a TCS SP8 confocal laser-scanning microscope (Leica). Excitation/emission settings were 488 nm/498-524 nm HyD1 SMD hybrid detector for CAH4 ...
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bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin-embedded tissue was sectioned into approximate 0.5cm3 cubes and then cut into 5-μm slices by a LEICARM2235 Slice Machine (Leica, Germany). The samples were stained with haematoxylin/eosin (H/E ...
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bioRxiv - Pathology 2023Quote: ... 5 μm thick sections were taken from the paraffin blocks using a rotary microtome (Leica RM2155, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin-eosin (HE) ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
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bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
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bioRxiv - Biochemistry 2022Quote: ... serial 5 μm thick sections of adult scallop’s eye were obtained using a Leica CM1950 freezing microtome (Leica, Wetzlar, Germany) for Raman spectroscopy testing (alpha300R ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
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bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged either with a Zeiss Observer A1 microscope and the AxioVision software 4.8 or with a STELLARIS 5 confocal microscope (Leica, Germany) at 40x (HC PL APO 40x/1.30 OIL CS2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 μm or 0.5 μm step sizes was performed on a Leica TCS sp8 Lightning Confocal/STED microscope coupled to LasX software (Leica) using a 63x (1.4 ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and 18.5 counting images were acquired using Leica Application Suite X (LAS X) on a Leica DMI8 fluorescent microscope from Leica Microscopy ...
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bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Neuroscience 2024Quote: ... Brains was postfixed overnight at 4 °C in a 4 % formaldehyde solution and then embedded in 5 % agarose gel for sectioning (50-µm-thick coronal sections) with a vibratome (VT1000S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... at 5 µm-thickness for paraffin samples or in a cryostat at 30 µm-thickness for frozen samples (Leica, Germany).
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned to a thickness of 7 µm for the testis and 5 µm for the epididymis using an Ultrathin Microtome from Leica, Germany ...
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bioRxiv - Plant Biology 2024Quote: ... The protein patterns of AP2:VENUS and SOC1:GFP under continuous LDs at the SAM were acquired with a Stellaris 5 confocal microscope (Leica) for fluorescence quantification (Fig ...
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bioRxiv - Immunology 2024Quote: The quality of cells and grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 547 nm laser line (560-580 nm emission) ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 3-5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Microbiology 2024Quote: ... 3-3.5 μL) were applied to glow-discharged lacey carbon grids and plunge frozen using an EM GP Plunge Freezer (Leica). Grids were positively glow-discharged using amylamine for the Hyper2 sample ...
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bioRxiv - Immunology 2024Quote: ... Sections were paraffin embedded by the histological core at the RI-MUHC and cut into 5 µm section using a microtome (Leica). Sections were deparaffinized in sequential xylenes followed by serial hydration steps in descending concentrations of ethanol ...
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bioRxiv - Neuroscience 2024Quote: Positioning and fluorescence of VLPs/cells on grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems). Z-stacks of desired regions were acquired in confocal-scan mode with a 490 nm laser (505-540 nm emission) ...
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bioRxiv - Immunology 2024Quote: ... 5 µm formalin fixed paraffin-embedded sections were stained with Akoya Biosciences Opal multiplex automation kit reagents (Leica Cat#ARD1001EA) on a Leice BondRX autostainer ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Cancer Biology 2024Quote: IHC of MPO was performed using 5-micron paraffin embedded sections on a Leica Bond RX auto stainer (Leica Biosystems). MPO primary antibody was diluted to 1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... filaments with three or more cells of 5-day-old protonemata regenerated from protoplasts were imaged using a wide-field microscope (DMI4000B, Leica). The length of subapical cells was analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence signal was recorded between 410 nm and 600 nm after excitation at 405 nM in 5 nm increments using a confocal microscope (Leica TCS SP8-AOBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the cocoon and all the fibers were observed using scanning electron microscopy (SEM, Hitachi, Japan) at an acceleration voltage of 5 kV and a polarizing optical microscope (Leica, Germany). For transmission electron microscope (TEM ...
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bioRxiv - Developmental Biology 2021Quote: ... The entire cerebellum of each animal (N=5 analyzed) was sectioned coronally at 50μm thickness on a vibratome (Leica VT 1000S) and all sections were processed for immunohistochemistry using a human nuclear antigen antibody (HuNu ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Neuroscience 2020Quote: CGEs from E14.5 Plxna4+/+;Htr3a-GFP or PlxnA4-/-;Htr3a-GFP coronal acute brain slices were microdissected under a fluorescence dissecting scope (Leica M165FC) and cells dissociated with 0.25% trypsin-EDTA (Life Technologies) ...
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bioRxiv - Bioengineering 2020Quote: ... The diffusion experiments were performed in the environmentally controlled chamber (set at 37 °C, 5% CO2) of a fluorescent microscope (Leica, CTR6500). The dextran solution was perfused inside the microvessel channel and images were acquired every 30 sec over 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Genomics 2021Quote: ... Serial sections cut to a thickness of 5 μm were obtained using a microtome (Leica RM2125RT, Leica Microsystems Nussloch GmbH, Germany) and stained in Gill 2 hematoxylin (Thermo Scientific Kalamazoo ...
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bioRxiv - Microbiology 2021Quote: ... collecting ∼200 000 bright-field images at 5 ms resolution with a 100× oil objective on a Leica DMi8 inverted microscope (Leica, Germany) at a set temperature value of 30°C.
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bioRxiv - Microbiology 2021Quote: ... Images of the colony patterns were taken under a 5× objective using a Leica DM6000B fluorescence microscope (Leica Corporation, Wetzlar, Germany) equipped with a LED fluorescence illuminator (Leica Corporation) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were placed in an incubator chamber at 37 °C with 5% CO2 supplementation mounted on a Leica TCS SP8X microscope system (Leica Microsystems). Laser micro–irradiation was performed using a protocol adapted from (6) ...
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bioRxiv - Cell Biology 2021Quote: ... and xz images were acquired in the presence of the membrane dye CellMask (5 mg/ml, Life Technology) using Leica TCS SP5X confocal microscope (Leica Microsystems). The MFI of Fluo-4 in the cytoplasmic region in individual cells was measured using the NIH ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).
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bioRxiv - Bioengineering 2022Quote: The liver with repopulated bile ducts of GFP-expressing cells was cultured in the circulation culture system for 5 days and imaged using confocal microscopy (Leica Microsystems). A reconstructed 3D image was prepared from Z-stack images using ImageJ (National Institutes of Health).
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bioRxiv - Microbiology 2020Quote: ... were visualized in the phase-contrast mode with a Leica DMi8 microscope with a 20X objective and an attached environment control chamber (37°C with 5% CO2) (Leica Microsystems). For each Transwell ...