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Citations for Leica :
751 - 800 of 888 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Z-stacks of zebra finch SETBP1 and co-localization experiments in Area X were acquired with a SP8-1 confocal microscope (Leica). All microscope settings were kept constant for all conditions and slices ...
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bioRxiv - Neuroscience 2024Quote: ... Z-stack were taken at 1 μm intervals using a 40x objective lens (W CORR CS2, NA: 1.10, Leica, Germany) on the Stellaris8 microscope (Leica ...
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bioRxiv - Developmental Biology 2024Quote: ... Included tendon constructs were then frozen at -80°C in isopentane for 1 minute and 12µm cryosections were generated with a CM3050 S Cryostat (Leica Biosystems).
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bioRxiv - Microbiology 2024Quote: ... Washed cell pellets were resuspended in a total volume of 50 µl PBS and 10 µl of each sample was spotted onto 1 % agar pads and subsequently imaged using the Thunder imager 3D cell culture microscope (Leica). First ...
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bioRxiv - Physiology 2024Quote: ... and covered with #1 thickness cover glass (Fisherbrand, cat#12-545-F) using a histological automaton (Leica Biosystem, cat#ST5020). Negative controls were treated with 1% BSA in PBS without the primary or secondary antibodies.
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled GFAP and EM48 fluorescent images were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Blinded images were opened in FIJI/ImageJ and condensed using Z project feature at maximum intensity ...
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled EM48 fluorescent images from the striatum were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Images were blinded ...
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bioRxiv - Neuroscience 2024Quote: ... and then a 1-mm-thick coronal slice containing the entire SCN was prepared using a vibratome (VT 1200S, Leica). Following this ...
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bioRxiv - Bioengineering 2024Quote: The specimens were immersed in an external cryoprotectant solution (1-hexadecene) and gently placed into HPF sample carriers (depth: 0.2 mm, Type A, Leica 16770141). The cover (Type B ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Briefly, MHC-II (clone V1030, dilution 1:500; Biomeda, Foster City, CA, USA) was revealed using Novolink Polymer (Leica Biosystems). Slides were then decolored using ethanol and the previous antibody was stripped using a β-mercaptoethanol/SDS solution in a waterbath preheated at 56°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibody was used: anti CD31 (CST, 3528, clone 89C2, 1:1600, antigen retrieval Leica ER1 AR9961 for 30min).
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bioRxiv - Cell Biology 2020Quote: ... Samples were mounted laterally in 1% agarose and imaged with a 10x objective lens on a SP5 confocal microscope (Leica Microsystems).
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Animals were observed under blue-light-cut illumination by a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and recorded in an H264 compressed movie6 ...
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bioRxiv - Bioengineering 2021Quote: ... The experimental setup consisted of (1) our microfluidic device installed on a Leica inverted microscope (DMIL LED Inverted Routine Fluorescence Microscope, Leica, Germany) and imaged using a colour camera (MC170 HD ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.
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bioRxiv - Plant Biology 2022Quote: ... seven-day-old Arabidopsis seedlings were incubated in 150 mM NaCl or 50 μM DNP for 1 hour and were then rapidly frozen with an EM ICE high-pressure freezer (Leica Microsystems).
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bioRxiv - Developmental Biology 2022Quote: ... A second planchet was placed over the first and then the sandwich was immediately frozen in a Leica EMPact 1 High Pressure Freezer (Leica Microsystems). The two halves of the planchet were then separated under liquid nitrogen to expose the tissue within the cavity ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Neuroscience 2020Quote: ... The signals of Dicer 1 and B2 Mm2 RNA and images of cells were obtained with a fluorescent microscope(DMi8, Leica Biosystems). The following probes were used:B2 Mm2 RNA probe 2 ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μm paraffin sections were prepared and processed on a Leica BOND Rx automated stainer using citrate-buffer based heat-induced antigen retrieval solution (Epitope Retrieval Solution 1 Bond, Leica, Germany) and DAB-based detection (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... elegans behaviors were recorded at 20 frames/s through a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and a CCD camera (1940 × 1460 pixels ...
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bioRxiv - Developmental Biology 2021Quote: ... ten fixed larvae were randomly selected from each culture (Dpf 1-24) and photographed under a microscope (Leica with DFC295 camera). Body length (BL ...
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bioRxiv - Cancer Biology 2020Quote: ... applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800, Leica Biosystems). Rabbit primary antibodies were incubated for either 15 or 30 minutes followed by detection with Refine DAB Polymer ...
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bioRxiv - Microbiology 2020Quote: ... were positioned on preheated flat agar plates (1% agar in egg water with 0.017% MS-222) and imaged with Fluorescence Stereo Microscope (Leica M205 FA). The image acquisition settings were as following ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Neuroscience 2023Quote: ... cut into 2 cm x 2 cm x 1 cm (LxWxH) tissue blocks using lancets and sectioned into 80 µm coronal sections on a microtome (Leica SM2010R). Brain sections were stored in cryoprotectant at -20 °C and washed in PBS at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... one ending of the tube was connected to the tip of a syringe (cut from a 1 ml syringe; Braun, 9166017V) which held the perfusion needle (Leica, 39471024), and the other ending was immersed in the solution chamber where the animal was placed ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture was incubated at room temperature for 5 min and 1 μL of each sample was pipetted onto a glass dish and imaged using THUNDER Imaging Systems (Leica Microsystems).
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Plant Biology 2023Quote: ... The transformed onion epidermal cells were inoculated on 1/2MS agar plates for ∼12h in the dark and then visualized under a confocal microscope (Leica SP8) for YFP signals ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with 1 µM siR-DNA solution and subsequently migratory behavior of the cells was recorded under a confocal-microscope (Leica SP8) for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Plant Biology 2023Quote: ... 1-Butanol was gradually replaced with melted paraffin wax (Surgipath Paraplast X-tra Tissue Infiltration/Embedding Media; Leica Biosystems, Wetzlar, Germany) using 1-butanol:paraffin (3:1) ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Developmental Biology 2024Quote: ... The individuals were first anesthetized (with MS-222, at a concentration of 150 mgL−1) and then photographed under a stereoscopic microscope (Leica, Germany) to measure the TL ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...