Labshake search
Citations for Leica :
751 - 800 of 1014 citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63x/1.40 oil objective (Leica). Multichannel time-lapse images were acquired in 4 fields each with 4 averages per frame in 1-min or 1.5-min intervals ...
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bioRxiv - Immunology 2021Quote: ... Organs included in paraffin were cut to a thickness of 5 microns (Microtome Leica RM2245 of disposable metal blades) and placed in a flotation solution in a water bath and then fixed on a slide sheet ...
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bioRxiv - Plant Biology 2020Quote: ... The embedded blocks were transversely sectioned at 5 to μm thickness using a Leica RM2125RT rotary microtome (Leica Microsystems) on RNase-free polyethylene naphthalate PEN membrane slides (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Physiology 2022Quote: ... The samples were imbedded in paraffin followed by serial sectioning at 5 µM thickness using the Leica microtome (Leica). The sections were deparaffinized ...
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bioRxiv - Biochemistry 2023Quote: ... The paraffin-embedded tissues were sectioned (5 µm thick) with a semi-automated rotary microtome (Leica Microsystems, Wetzlar, Germany) and placed on microscope glass slides coated with poly-L-lysine ...
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bioRxiv - Zoology 2023Quote: ... Paraffin blocks were sectioned (5-7 μm, 20-40 sections per block) using a microtome (Leica RM 2125RT, Germany). Histological sections were stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Bioengineering 2023Quote: ... the grips were attached to a custom mechanical testing rig mounted on an upright confocal microscope (Leica STELLARIS 5) in a bath with 1× PBS (Fig ...
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... PL Apo 40x/0.85 CORR CS and PL APO 63x/1.40 oil CS2 and Leica Stellaris 5 confocal microscopes (Leica Microsystems) equipped with a HCPL FLUOTAR 10x/0.30 ...
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bioRxiv - Neuroscience 2024Quote: ... saturated with 95 % O2 and 5 % CO2 to obtain 250 μm-thick coronal sections with a vibratome (Leica VT1200S). Slices were transferred and incubated in a holding chamber containing (ACSF ...
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bioRxiv - Biophysics 2023Quote: ... They were imaged with sequential z-stacks using a similar procedure as for the 3D colonies but with an 5× air objective (Nplan5×0.12PHO, Leica). The voxel size in (x,y,z ...
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bioRxiv - Developmental Biology 2024Quote: Fixed samples were imaged in a Leica Stellaris 5 upright confocal microscope using a HC PL APO 40x/1,1 Water CORR CS2 objective (#506425, Leica). The microscope was operated by the LAS X (v4.5.0.25531 ...
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bioRxiv - Immunology 2024Quote: ... The stage-top chamber was set onto the stage of a Stellaris 5 confocal microscope (Leica Microsystems, Deerfield, IL). Transmitted (brightfield ...
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bioRxiv - Cell Biology 2019Quote: ... submersed in the media chamber containing 4°C Phosphate buffered Saline and cut using a Leica VT1200S vibrating blade microtome (Leica Biosystems, UK) at a speed 0.3mm/sec ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... A series of 4-μm thick longitudinal sections and transverse sections (rapidly elongating region) were made with a rotary microtome RM2265 (LEICA EM UC7). Fixed sections were stained with toluidine blue O for 30 min and photographed with an Olympus BX51 microscope equipped with a DP74 camera.
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were postfixed in 4% paraformaldehyde overnight on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S), with sections collected into six tubes (containing cryoprotectant solution as described[32] ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... The embedded tissue blocks were serially cut into 5-μm-thick ground sections using a microtome (Leica SM 2000; Germany) and mounted on glass slides ...
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bioRxiv - Neuroscience 2019Quote: ... a minimum of 5 fields of view were examined for each of 3 sections in both hemispheres using a LASX (Leica) widefield microscope or a SP5II (Leica ...
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bioRxiv - Neuroscience 2019Quote: ... Imaging was performed for 5min at 1Hz at 37°C 5% CO2 on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40 ...