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Citations for Leica :
701 - 750 of 1094 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then embedded in low melting point agarose (σ Type VIIA, 3% in Ames medium) and 250 µm slices were obtained using a vibratome (Leica Microsystems, VT1200S) in cold (∼4° C) ...
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bioRxiv - Neuroscience 2024Quote: ... the sucrose solution was removed and organoids were placed on OCT (at least 3 organoids on each OCT drop) and cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 20 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of GN2 or scraped cells were placed between two 3 mm diameter aluminum- or gold-coated (Type A) specimen carriers with 100 µm indentations (Leica Microsystems Inc., Austria). HT29 cells directly grown on the same type of 3 mm diameter aluminum- or gold-coated (Type A ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Molecular Biology 2021Quote: ... The paraffin blocks transferred to room temperature and the blocks sections cut 4 mm thick in a rotatory microtome (Leica RM2135) and mounted in glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were removed and post-fixed in PBS containing 4% paraformaldehyde over night before cutting 50μm thick coronal sections on a vibratome (VT 1000 S from Leica, Wetzlar, Germany). Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Neuroscience 2020Quote: Brains were embedded in agar (3-4% w/v dissolved in dH2O) before being cut into 50 μm coronal sections on a vibrating microtome (VT1000S; Leica Microsystems). Free-floating tissue sections were collected in series ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
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bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted from the skull and stored in 4% formaldehyde before being cut in sagittal sections (50 μm) using a vibratome (Leica VT1000) and stained with cresyl violet ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Bioengineering 2021Quote: ... according to standard histological procedures and 4 μm thick sections of the paraffin blocks were cut on the microtome (Leica, HM325). The paraffin sections were stained with Haematoxylin-Eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
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bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused with 4% PFA in PBS and brains were removed and sectioned coronally or sagittally on vibratome (Leica VT1000) at 50-75 μm and mounted on slides with ProLong(tm ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde in PBS and imaged using laser scanning confocal microscopy (LEICA DMi8, Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...