Labshake search
Citations for Leica :
701 - 750 of 1277 citations for 6 Bromo 3 4 dihydro 2H 1 benzothiin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... Positive nuclei (Ki67) and stained area (cleaved caspase 3) were analyzed using the ImageScope software (Leica Biosystems). Quantification is average of 3 sections ...
-
bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
-
bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
-
bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
-
bioRxiv - Cell Biology 2022Quote: ... and images were acquired using a 3-channel TCS SP2 laser-scanning confocal microscope (Leica Mycrosystems, Germany) and STELLARIS 8 ...
-
bioRxiv - Molecular Biology 2023Quote: 2.8 × 105 A549 or A549-IFITM3 cells were seeded on carbon- coated 3 mm sapphire discs (Leica) in a 6-well plate in complete growth medium (DMEM-F12 ...
-
bioRxiv - Neuroscience 2023Quote: ... the puncture sample was placed in an aluminum specimen carrier (Ø = 3 mm; Leica Microsystems, Wetzlar, Germany) and underwent freezing in a high-pressure freezer (HPM100 ...
-
bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
-
bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
-
bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
-
bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the possible contact sites were re-embedded into resin blocks and 70 nm thick sections were cut with an ultra-microtome (Leica EM UC 6). Electron microscopic images were taken either with a Hitachi H-7100 transmission electron microscope or a FEI series electron microscope to reveal synaptic contacts between BFPVNs and target cell types in the MS ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
-
bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
-
bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
-
bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...
-
bioRxiv - Neuroscience 2022Quote: ... then following fixation they were imaged with an inverted confocal microscope with the laser power increasing for 0.2 to 3% with 0.2% intervals (Extended Data Fig. 1a, b, SP8 TCS with HyD detectors, Leica). Laser power was linearly controlled by ATOF ...
-
bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
-
bioRxiv - Plant Biology 2022Quote: ... GUS-stained leaves were embedded in 3% agarose and sectioned with a vibrating blade microtome (Leica VT1000 S). Images were taken using ZEISS Axio Observer.
-
bioRxiv - Neuroscience 2023Quote: ... the ISH-mounted specimen was first loaded into a pre-tilted specimen block (Leica; Figure 3 J&K). The block was then placed into a Leica VCT-100 cryo-transfer system under liquid nitrogen to facilitate transfer into the pre-cooled cryo-FIB/SEM cryo-substage.
-
bioRxiv - Cell Biology 2023Quote: ... The cryosection ribbon (∼3 mm long) was then attached to the grid by operating the Crion (Leica Microsystems) in “charge” mode for ∼30 seconds (Pierson et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in blocks of 3% agarose and sectioned in 100 µm slices on a vibrating blade microtome (Leica) or mounted as whole droplets.
-
bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33 ...
-
bioRxiv - Cell Biology 2021Quote: ... was deposited on an EM copper grid (400-mesh, Plano G2400C, coated with 6 nm thick carbon produced with a Leica EM ACE600 sputter coater), incubated for 30-60 sec and manually blotted with filter paper ...
-
bioRxiv - Microbiology 2021Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
-
bioRxiv - Immunology 2023Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
-
bioRxiv - Cancer Biology 2021Quote: ... Each wash was performed for 3 min at room temperature with repetitive dipping using a linear stainer (Leica, #ST4020). Slides were transferred for heat-induced epitope retrieval to a PT module (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2021Quote: Fertilized ovaries from B73 plants were collected 3 days after pollination (DAP) and sliced using a Vibratom (Leica VT1000E) to create 200 to 225 μm sections ...
-
bioRxiv - Cancer Biology 2020Quote: ... two sections of formalin-fixed paraffin-embedded tissues of FMC with 3 µm thickness (Microtome Leica RM2135, Newcastle, UK) were prepared ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
-
bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
-
bioRxiv - Neuroscience 2021Quote: ... Three (3) brains per group were later sectioned with a cryostat (Cryocut 1800 Cryostat, Leica Biosystems, Vista, CA, USA) at 25 μm thickness in the coronal plane ...
-
bioRxiv - Immunology 2020Quote: ... Lung tissues were embedded in 3% LMA and 100μm-thick sections were cut at the vibratome (Leica VT 1200S). Sections were placed on microscope slides ...
-
bioRxiv - Plant Biology 2020Quote: ... cinerea conidia and germlings exposed to 3 μM NCR044.1 was also imaged using fluorescence confocal microscopy (Leica SP8-X)39 with an excitation and emission wavelength of 480 and 510-566 nm ...
-
bioRxiv - Genomics 2021Quote: ... Formalin-fixed agarose/paraffin-embedded sections (3-5µm) were manually cut from blocks with a microtome (Leica RM 2145) and transferred to glass slides (Superfrost ...
-
bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
-
bioRxiv - Developmental Biology 2022Quote: ... Blastema tissues (n=3 for neotenic and metamorphic animals) were first embedded in a tissue freezing medium (Leica, 14020108926). Then ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
-
bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
-
bioRxiv - Biophysics 2022Quote: ... 300 mesh grids were coated with a continuous carbon layer of 3 nm in sputter coater EM ACE600 (Leica). The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11 ...
-
bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
-
bioRxiv - Neuroscience 2023Quote: ... and cortex region (n=3/group) were cut at 5μm thickness in a rotary microtome (Leica RM2125RT, Wetzlar, Germany) for histological analysis (Venkidesh et al. ...
-
bioRxiv - Biophysics 2023Quote: ... We image z-stacks around single cells with a cubic volume of (123μm)3 using confocal reflection microscopy (Leica DM 6000 CFS with HCX APO L 20x/1,00 W objective) ...