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Citations for Leica :
701 - 750 of 1490 citations for 6 BROMO 3 4 BROMO 2 METHYL PHENYL ISOQUINOLIN 1 YLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: Brains of mice (n = 4 per group) perfused with 4% PFA/PBS were serially cut into 50 μm-thick coronal sections using vibratome (VT1200S, Leica), and the sections from one cohort of mice (a littermate pair of wild-type and Bdnf+/- mice ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Evolutionary Biology 2021Quote: ... 300µm-thick acute coronal brain sections were cut after embedding in 4% low melt-agar using a vibratome (Leica, #VT1000S) under RNase-free conditions ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Neuroscience 2020Quote: ... the slices were embedded in 4% low melting agarose and cut in 70µm thick sections in a vibrating microtome (Leica, VT1000S). Sections around the tip of the recording electrode were collected and cryoprotected in 10% and 20% sucrose solutions in PB ...
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bioRxiv - Physiology 2021Quote: ... then individually embedded in paraffin blocks and sectioned in serial sagittal sections (4 μm) using a rotary microtome (Leica, Germany). Tissue sections were permanently mounted ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Color-coded time projections were generated in ImageJ using a publicly available plugin (K ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TauRDP301S-CFP/YFP and HEK293 TauRDP301S-CFP cells treated with Tau condensates were fixed with 4% PFA in PBS prior to confocal FLIM on a Stellaris 8 FALCON (Leica) equipped with a 100x objective ...
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bioRxiv - Genomics 2022Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Microbiology 2022Quote: ... the eyes were cut into 4 μm sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... images were taken as a time series on an xy plane across 6 z planes at an interval of 4 s using a 10× objective with an NA of 0.4 and an optical zoom of 4 on an SP5 inverted confocal microscope mounted with a resonant scanner (Leica Microsystems). The z project across time was then obtained as a time series.
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bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
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bioRxiv - Cell Biology 2021Quote: ... brains fixed in 4% paraformaldehyde (PFA) were embedded in paraffin and sectioned 5µm thick using a microtome system (Leica RM2165). Immunohistochemistry was performed on brain sections by permeabilizing them with TBS-0.5% Triton-X (TBS-Tx ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Cancer Biology 2020Quote: Paraffin tissue-blocks were serially sectioned with slices thickness of 4 µm on a microtome (Leica Byosystem, Buffalo Grove, IL) and allowed to dry at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed in 4% PFA overnight and coronally sectioned at 75 µm on a Vibratome (Leica VT1000 S). All slices were then mounted on Superfrost Plus slides using Fluoromount-G mounting medium (Southern Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... then transcardially perfused with 10% sucrose in phosphate buffered saline (PBS) followed by 4% paraformaldehyde in PBS for approximately 90 s each using a Perfusion Two system (Leica). The brain was then removed and postfixed in 4% paraformaldehyde in PBS overnight at 4°C before being moved to a 30% sucrose solution in PBS for cryoprotection ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... The OE were embedded in 4% low melting point agarose blocks and sectioned (30 µm) using a Vibratome (VT 1200; Leica). The sections were blocked in 1% blocking solution (10 min at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were cut coronally into 15-μm sections and mounted in 4 series using a cryostat (Leica CM1950, Nussloch, Germany) to obtain 16 matching sections containing the DR (−7.3 to −8.7 mm from bregma ...
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bioRxiv - Plant Biology 2019Quote: ... Nodules were embedded in 4% agarose and 50-100 μM sections were made with a vibratome® 1000 plus (Leica). Sections were immersed in a staining solution (2.5% 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic (X-Gluc) ...
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bioRxiv - Neuroscience 2019Quote: ... 2.5 mM KCl, 25 mM glucose, 1.25 mM NaH2PO4, 4 mM MgCl2, 0.5 mM CaCl2, and 24 mM NaHCO3, Leica VT1200 vibratome). The slices were incubated at 32 °C for 60 minutes after sectioning and were then stored at room temperature until they were used ...
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bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum containing hNSCs (equivalent to +1.4 to +0.14 mm from Bregma) (Franklin and Paxinos, 2007) was cut at 60 μm using a vibratome (Leica Microsystems). After pre-embed IHC of the striatum using diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed heads were embedded in 4% low gelling agarose and 200 µm sections were obtained using a vibratome (Leica, VT1200S). Sections were post-fixed with 1% osmium tetroxide in water ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin embedded sections were then sliced into 4 μm-thick slices on a Leica RM2016 Microtome (Leica, Shanghai, China) for immunohistochemistry staining and apoptotic cells labeling ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 4% agarose before being cut into 40 um coronal slices with a vibratome (Leica VT S10000) and stored in 0.1% PBS Sodium Azide ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v) sucrose for two days and then embedded in tissue freezing medium (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections ...
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bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then coated with a 4 nm layer of platinum using a ACE600 high resolution sputter coater (Leica Microsystems) before being imaged using an FEI Quanta 450 ESEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Molecular Biology 2023Quote: ... Immunohistochemistry or immunofluorescence experiments were performed on 4 µm-sections using Novolink polymer detection system (7140-K, Leica Micro-systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...