Labshake search
Citations for Leica :
651 - 700 of 1108 citations for Rat Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Brains were mounted in 2% agarose and sectioned at 50 μm using a vibratome (Leica VT1000s). Coronal sections were used for all brains ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in 2% agarose and sectioned to 50-μm coronal/sagittal sections by a vibratome (Leica). For OE sections ...
-
bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections (2 μm thickness) obtained with a Leica RM2245 rotary micro-tome (Leica Biosystems) were mounted on glass slides (Superfrost Plus ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica, cat# AR9640). Both mouse monoclonal anti-Ki67 CloneMIB-1 (Dako ...
-
bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
-
bioRxiv - Plant Biology 2021Quote: ... using a HistoResin embedding kit (Leica, www.leica-microsystems.com), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... The Bond Refine polymer staining kit (Leica Biosystems) was used ...
-
bioRxiv - Immunology 2021Quote: ... with Bond Polymer refine detection kit (Leica, DS9800). Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (Leica ...
-
bioRxiv - Genomics 2021Quote: ... using the Enzyme Pretreatment Kit (AR9551; Leica Biosystems) and followed by a counterstaining with Hematoxylin (AR9551 ...
-
bioRxiv - Cell Biology 2022Quote: ... a Novolink Polymer Detection Systems Kit (RE7280K; Leica Biosystems Newcastle L10 ...
-
bioRxiv - Immunology 2023Quote: ... utilizing the Bond Research Detection kit (Leica Microsystems) and Opal fluorophores (Akoya Biosciences) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The Bond Refine staining kit (Leica Microsystems DS9800) was used ...
-
bioRxiv - Genetics 2021Quote: FFPE tissue sections were cut using a microtome and baked at 62°C for 20 minutes followed by haematoxylin and eosin (H&E) staining using a Leica Autostainer XL (Leica Microsystems, Wetzlar, Germany). Slides were mounted with DPX (distyrene ...
-
bioRxiv - Molecular Biology 2020Quote: ... The nuclei were imaged using a Leica DM6 B microscope equipped with Leica DMC5400 camera or DM IL LED fluorescence microscope equipped with Leica DFC450 C (Leica Microsystems, Wetzlar, Germany) camera and the images were processed using ImageJ software version 1.53 (National Institutes of Health ...
-
bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
-
bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
-
bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
-
bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
-
bioRxiv - Microbiology 2020Quote: ... While keeping the orientation succession the slices were further processed into paraffin blocks using the Leica ASP6025 tissue processor and Leica EG1150H+C tissue embedding centre (both Leica Biosystems, Nussloch, Germany). Three serial adjacent 2.5 μm thick sections were then cut with the Leica RM 2255 Microtome (Leica Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
-
bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
-
bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
-
bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
-
bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
-
bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
-
bioRxiv - Microbiology 2021Quote: ... Polymerization of these epoxy blocks was performed at 70 °C and then they were sectioned with an untramicrotome (Leica microsistems Gmbh, Wein Austria). Very small small sections of 80 nm thickness were collected on a cupper grid of 200 mesh (pore size ...
-
bioRxiv - Neuroscience 2019Quote: ... brains were removed from the skull and 300μm-thick parasagittal slices (horizontal for thalamic stimulation experiments) were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Individual slices were then submerged in a recording chamber mounted on the stage of an upright BX51WI microscope (Olympus ...
-
bioRxiv - Neuroscience 2019Quote: ... the neurons at the end of treatment were stained with JC-1probe solution at 37 °C for 20 min and observed under fluorescence microscope (DMi8, Leica Biosystems, Wetzlar, Germany). The mitochondria were also extracted by a mitochondrial extraction kit ...
-
bioRxiv - Plant Biology 2019Quote: ... The abaxial side of the leaf samples was imaged using a 63×/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). FLIM experiments were performed using a Leica TCS SP8X confocal microscope equipped with TCSPC (time correlated single photon counting ...
-
bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
-
bioRxiv - Cell Biology 2021Quote: The epithelium at ALI were incubated at 37°C and 5% CO2 in the 3i Marianis/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA) and high speed time-lapse videos were taken at 32X air at 100 Hz with a total of 250 frames using a scientific Hamamatsu C1140-42U30 CMOS camera (Hamamatsu Photonics ...
-
bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
-
bioRxiv - Cell Biology 2022Quote: ... Vials were loosely capped and transferred to the chamber of the freeze substitution machine that contained about ½ inch-deep bath of ethanol to improve thermal conductivity and was pre-cooled to -112°C (EM AFSII, Leica Microsystems, Wetzlar, Germany). Within about 10 minutes in the freeze substitution machine ...
-
bioRxiv - Immunology 2022Quote: Cryo-fluorescence microscopy samples were transferred to the cryo-FIB-SEM microscope in liquid nitrogen using a Leica EM-VCM500 machine and a cryo-holder suitable for c-clipped grids (Leica Microsystems, Vienna, Austria). Prior to sample loading into the FIB-SEM microscope ...
-
bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
-
bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 12 µm in the coronal plane with a Leica CM1860 UV cryostat (Leica Biosystems, Wetzlar, Germany). Sections corresponding to Atlas plates 20-26 of Paxinos (Bregma +1.34 to +0.62 mm ...
-
bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
-
bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
-
bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
-
bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
-
bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
-
bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently transferred into a Leica EM AFS-2 freeze substitution unit (Leica Microsystems). Over a period of five days ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
-
bioRxiv - Physiology 2021Quote: ... CFP and DsRed fluorescence signal were detected after 2 days with a CLSM (Leica AF6000 modular microsystems). All experiments were biologically repeated for three times.