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Citations for Leica :
651 - 700 of 1185 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 1A) and placed under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
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bioRxiv - Neuroscience 2020Quote: ... Extracted brains were then sectioned into 50 μm-thick free-floating coronal slices using a semi-automatic vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were stored in PBS at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... fixed brains were transferred in PBS and sectioned into 50-um thick coronal slices on a Leica vibratome (Leica Microsystems GmbH, Vienna, Austria). Free-floating slices were placed in tris-EDTA buffer (pH 9 ...
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bioRxiv - Plant Biology 2022Quote: ... Ultrathin sections of 50 nm thick were cut transversally at 1.8mm from the root tip using a Leica Ultracut (Leica Mikrosysteme GmbH, Vienna, Austria), picked up on a copper slot grid 2 x 1 mm (EMS ...
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Functional states of prelimbic and related circuits during the acquisition of a GO/noGO task in ratsbioRxiv - Neuroscience 2024Quote: ... Brains were cryoprotected with a PBS solution of 30% sucrose for a week before cutting them in coronal sections (50 µm) with the help of a sliding freezing microtome (Leica SM2000R, Nussloch, Germany). Sections including the implanted sites were mounted on gelatinized glass slides and stained ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950, Leica Biosystems, Wetzlar, Germany) for a total duration of 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... All slices were embedded in 20% gelatin and further cut into 50 μm-thick sections in ice-cold PB using a vibratome (VT1000S, Leica Microsystems, Wetzlar, Germany). After sectioning ...
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bioRxiv - Zoology 2024Quote: ... The resin blocks containing the samples were polymerized for 36–60 hours at 60°C and then were cut into ultrathin sections of 50–80 nm with a diamond knife on an ultramicrotome (EM UC7, Leica Microsystems, Wetzlar, Germany). The sections where then viewed under a transmission electron microscopy (H-7650 ...
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bioRxiv - Immunology 2022Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: The immunohistochemically stained slides were scanned using a ScanScope XT Slide Scanner (Leica Aperio Technologies, Vista, CA, USA) under 20x objective magnification (0.5μm resolution ...
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bioRxiv - Physiology 2024Quote: ... Whole slide images of antibody-labeled tissues were captured on an Aperio VERSA 200 (Leica Biosystems, Vista, CA) at the Vanderbilt Digital Histology Shared Resource (DHSR) ...
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bioRxiv - Cancer Biology 2023Quote: ... F4/80 (Abcam ab6640, 1:100, Enzyme 1 Leica), CD45R (Abcam ab64100 ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were embedded in 3% agarose gel in a cubic cryomold and coronally sectioned at 50 μm thickness using a VT1000S vibrating blade microtome (Leica Biosystems, Buffalo Grove, IL). The sections were rinsed in PBS ON at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were thoroughly washed in 1x PBS and thereafter sectioned (50 μm thickness) using a vibratome (Leica VT1000, Leica Microsystems, Nussloch GmbH, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Three (3) brains per group were later sectioned with a cryostat (Cryocut 1800 Cryostat, Leica Biosystems, Vista, CA, USA) at 25 μm thickness in the coronal plane ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were embedded in OCT medium (Tissue-Tek; Sakura, Torrance, CA) and serially sectioned by cryostat (Leica, Wetzlar, Germany) into 40-μm coronal slices containing the LC and PFC ...
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bioRxiv - Cell Biology 2023Quote: WSIs were generated by scanning the glass slides with a whole slide brightfield microscopy image scanner (Aperio, Leica, CA). Spatial annotation of tissue sections was performed in Aperio®ImageScope and saved as ImageScope compatible XML files.
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bioRxiv - Cell Biology 2024Quote: Confocal images of HeLa cells and BOECs expressing G-Ca-FLITS were taken with a Leica Sp8 system (Leica) with a ×63 objective (HL PL Apo ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of the whole SN of Young 1 and Old 1 were obtained by sectioning the tissue (20 μm) on a freezing microtome (Leica, CM1850UV-1-1, Germany) at −24 °C.
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bioRxiv - Cancer Biology 2023Quote: ... and Ly6G (2B Scientific BE0075-1, 1:60000, ER2 Leica). Nkp46 and FoxP3 were stained on a Dako AutostainerLink48 ...
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bioRxiv - Neuroscience 2023Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
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bioRxiv - Microbiology 2023Quote: ... TTF-1 (Leica), IBA1 (Fujifilm) ...
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bioRxiv - Genomics 2021Quote: ... AT2 cell purity was determined by NKX2-1 cytospin staining (1:100, Leica Biosystems, Cat # NCL-1-TTF1). Donor1 was 83% positive ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Neuroscience 2023Quote: ... We then cut the spinal cord in 50 µm coronal sections on gelatinized slides using a cryostat (Leica CM1860, Leica Biosystems Inc, Concord, ON, Canada). Sections were mounted on slides and stained with 1% Cresyl violet ...
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bioRxiv - Cell Biology 2020Quote: ... elastic modulus and dissipation were performed on a Catalyst BioScope atomic force microscope (Bruker, Santa Barbara, CA) combined with a 3-channel confocal microscope TCS SP5 II (Leica; 20×/NA 0.70 or 40×/NA 0.85 air objectives) ...
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bioRxiv - Pathology 2021Quote: ... CA) was used with the Leica RNAScope 2.5 LS Assay-RED kit and a hematoxylin counterstain (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Physiology 2020Quote: Photographs of sections processed for mRNA in situ hybridization or immunohistochemistry were captured using a QIClickTM CCD Color Camera (Qimagin, British Columbia, CA) linked to a DMRA2 light microscope (Leica), utilising Volocity® v.6.3.1 image analysis software (PerkinElmer ...
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bioRxiv - Pathology 2022Quote: ... with RNAscope 2.5 LS Reagent Kit-Red (322150, Advanced Cell Diagnostics, Newark, CA) and Bond Polymer Refine Red Detection (DS9390, Leica Biosystems). Control probes detecting a validated positive housekeeping gene (mouse Ppib to confirm adequate RNA preservation and detection ...
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bioRxiv - Microbiology 2024Quote: ... Slides were scanned using Aperio AT Turbo (Aperio, Vista, CA, USA) and images extracted using Aperio Image Scope software v12.3.2.8013 (Leica Biosystems, Wetzlar, Germany). Immunohistochemistry was undertaken as described using the in-house developed anti-spike monoclonal antibody 1E830,31 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then put in a mold in OCT compound (SAKURA 4583, Sakura Finetek, Torrance, CA, USA) and left to freeze in a cryostat (Leica CM1950 ...
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bioRxiv - Cancer Biology 2022Quote: ... Ly6G (clone 1A8, 2B Scientific BE0075-1, ER2 Leica, 1:60000), LTF (Thermofisher ...
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bioRxiv - Pathology 2022Quote: ... CA) was detected using the Leica RNAScope 2.5 LS Assay-RED kit with a hematoxylin counterstain (#322150, Leica Biosystems, Richmond, IL). An RNApol2 probe served as a host gene control to evaluate RNA quality ...
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bioRxiv - Neuroscience 2023Quote: ... compound (Sakura Finetek, CA, USA) and cryo-sectioned into 10 µm thick slices using a cryostat (CM1860; Leica Biosystems, Nussloch, Germany), The sections were mounted on glass slides ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were first deparaffinized and then underwent antigen retrieval by incubating with EDTA-based epitope retrieval solution (BOND Epitope Retrieval Solution 2, Cas#AR9640, Leica BioSystems) for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Slides were scanned using Aperio AT Turbo (Aperio, Vista, CA USA) and analyzed by and Aperio Positive Pixel Count Algorithm (Leica Biosystems) that provided weak positive ...
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bioRxiv - Developmental Biology 2021Quote: ... 1/1000 (Leica biosystems), Rabbit anti-PH3 ...
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bioRxiv - Immunology 2024Quote: ... and #1 coverslips (Leica).
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bioRxiv - Developmental Biology 2024Quote: ... Aqueous eosin 1% (Leica) was manually applied onto sections with a pipette and rinsed with deionised water after 1-3 seconds ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-TTF1 (Nkx2.1) (1:100; Novocastra; Leica Biosystems NCL-L-TTF-1), mouse anti-NeuN (1:500 ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: ... Slides were scanned using Aperio AT Turbo (Aperio, Vista, CA USA) and analyzed using Aperio ImageScope software (Leica Biosystems, Mt Waverley, Australia) (v10 ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images were scanned using a Leica Aperio AT2 digital whole slide scanner and acquired using ImageScope viewing software (Leica Biosystems, Vista, CA). Microscopic findings were graded on the following scale ...
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bioRxiv - Microbiology 2020Quote: ... The other paraffin sections were stained with HPS on a Tissue-Tek Prisma autostainer (Sakura, CA, US) and microscopically examined (DM2500 microscope Leica, Vienna, Austria).
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bioRxiv - Biochemistry 2020Quote: ... High-resolution optical scans of the stained tissue sections were acquired using a Leica Aperio CS2 (Leica Biosystems Imaging, Vista, CA, USA).