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Citations for Leica :
651 - 700 of 774 citations for OX7 Anti Thy 1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.
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bioRxiv - Plant Biology 2022Quote: ... seven-day-old Arabidopsis seedlings were incubated in 150 mM NaCl or 50 μM DNP for 1 hour and were then rapidly frozen with an EM ICE high-pressure freezer (Leica Microsystems).
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bioRxiv - Developmental Biology 2022Quote: ... A second planchet was placed over the first and then the sandwich was immediately frozen in a Leica EMPact 1 High Pressure Freezer (Leica Microsystems). The two halves of the planchet were then separated under liquid nitrogen to expose the tissue within the cavity ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Neuroscience 2019Quote: ... Pupils were dilated with 1% (wt/vol) tropicamide (Bausch & Lomb, USA) to facilitate transpupillary visualization under an operating microscope (Leica, USA). For SRS transplantation ...
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bioRxiv - Neuroscience 2020Quote: ... The signals of Dicer 1 and B2 Mm2 RNA and images of cells were obtained with a fluorescent microscope(DMi8, Leica Biosystems). The following probes were used:B2 Mm2 RNA probe 2 ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μm paraffin sections were prepared and processed on a Leica BOND Rx automated stainer using citrate-buffer based heat-induced antigen retrieval solution (Epitope Retrieval Solution 1 Bond, Leica, Germany) and DAB-based detection (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Cancer Biology 2019Quote: ... the tip of a syringe (cut from a 1 ml syringe-Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) to the tubing ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... elegans behaviors were recorded at 20 frames/s through a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and a CCD camera (1940 × 1460 pixels ...
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bioRxiv - Developmental Biology 2021Quote: ... ten fixed larvae were randomly selected from each culture (Dpf 1-24) and photographed under a microscope (Leica with DFC295 camera). Body length (BL ...
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bioRxiv - Cancer Biology 2020Quote: ... applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800, Leica Biosystems). Rabbit primary antibodies were incubated for either 15 or 30 minutes followed by detection with Refine DAB Polymer ...
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bioRxiv - Microbiology 2020Quote: ... were positioned on preheated flat agar plates (1% agar in egg water with 0.017% MS-222) and imaged with Fluorescence Stereo Microscope (Leica M205 FA). The image acquisition settings were as following ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Neuroscience 2024Quote: ... the hippocampi of 1-month-old male mice were dissected and sliced transversally using a vibratome (model VT 1200S; Leica Microsystems) with a thickness of 300 µm ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture was incubated at room temperature for 5 min and 1 μL of each sample was pipetted onto a glass dish and imaged using THUNDER Imaging Systems (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... cut into 2 cm x 2 cm x 1 cm (LxWxH) tissue blocks using lancets and sectioned into 80 µm coronal sections on a microtome (Leica SM2010R). Brain sections were stored in cryoprotectant at -20 °C and washed in PBS at 4 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Bioengineering 2023Quote: ... one ending of the tube was connected to the tip of a syringe (cut from a 1 ml syringe; Braun, 9166017V) which held the perfusion needle (Leica, 39471024), and the other ending was immersed in the solution chamber where the animal was placed ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Plant Biology 2023Quote: ... 1-Butanol was gradually replaced with melted paraffin wax (Surgipath Paraplast X-tra Tissue Infiltration/Embedding Media; Leica Biosystems, Wetzlar, Germany) using 1-butanol:paraffin (3:1) ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed onion epidermal cells were inoculated on 1/2MS agar plates for ∼12h in the dark and then visualized under a confocal microscope (Leica SP8) for YFP signals ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with 1 µM siR-DNA solution and subsequently migratory behavior of the cells was recorded under a confocal-microscope (Leica SP8) for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 4μm sections of organoid blocks were stained with ARID1A antibody (ab182560, 1/3000) on the automated Leica BOND RX system (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Developmental Biology 2024Quote: ... The individuals were first anesthetized (with MS-222, at a concentration of 150 mgL−1) and then photographed under a stereoscopic microscope (Leica, Germany) to measure the TL ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Biophysics 2019Quote: Samples were freeze-substituted in 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica AFS (Leica Microsystems, Vienna, Austria) following a previously described protocol [52] ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were stained with phalloidin 647 and fluorescent z-stacks were acquired by confocal microscopy (Leica SP8, or Zeiss Examiner Z.1) at a pixel size of 60-70 nm ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Bioengineering 2020Quote: ... Observations of the samples were carried out under fluorescence microscope (Axio Imager D.1, Zeiss) and confocal microscope (Leica SP5 upright Confocal).
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Microbiology 2020Quote: ... Max projections of confocal z-stacks (25 z steps spaced 1 μm) were acquired on a SP5 resonant APD (Leica, Wetzlar, Germany) at 1.7x zoom using a 63x glycerol objective (numerical aperture 1.4).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Neuroscience 2021Quote: Every 4th brain section was imaged as a Z-stack (1 μm step size) using either the Zeiss LSM 800 (Zen, v2.6.76) or Leica SP8 confocal microscope (Leica Application Suite X, v3.5.6.21594) at 20x magnification ...