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Citations for Leica :
651 - 700 of 737 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections from electrophysiological or calcium imaging experiments containing the vH were examined with a fluorescent microscope (M205 FCA, Leica Microsystems, Wetzlar, Germany) using a 2.0x magnification objective ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was stored in −80 °C and sectioned from an OCT block at 12 µm thickness and mounted on Leica PPS-membrane Laser Microdissection (LMD) slides (Leica, Cat# 11505268). A rapid stain protocol23 prepared slides for LMD ...
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bioRxiv - Physiology 2023Quote: ... and islets were hand-picked from any residual non-islet tissue using a 20uL pipette under a light microscope (Leica DMIL LED, Leica, Wetzlar, Germany) and placed in 4mL of the RPMI-1640 medium in a 60mm petri dish (Falcon #351007 ...
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bioRxiv - Cell Biology 2023Quote: ... Semithin sections (250 nm) were cut from conventionally embedded vestibular organs on an Ultracut E or an UC7 ultramicrotome (Leica Microsystems, Germany) with a 35° diamond knife (Diatome AG ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal slices from the region of the somatosensory cortex (350 μm in thickness) were collected with a vibratome (VT 1200; Leica, Nussloch, Germany). Following the preparation of brain slices ...
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bioRxiv - Neuroscience 2023Quote: ... Hemispheres were separated and 300 µm/150 µm (field recordings/electron microscopy) thick sagittal sections were cut from both hemispheres with a vibratome (VT1200 S, Leica Biosystems (RRID:SCR_018453)) ...
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bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was removed from the skull and sliced at 250 μm using a vibrating microtome (VT1000s, Leica Biosystems, Buffalo Grove, IL) in cold ...
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bioRxiv - Cancer Biology 2024Quote: All stained sections except for those from experiments involving Lepob mice (Fig. 3) were scanned at 20x magnification using a Leica Aperio AT2 slide scanner (Leica Microsystems, UK) prior to subsequent analysis of scanned slides as described below.
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Bioengineering 2024Quote: ... three different positions per gel were acquired at the gel center from two independent samples using a confocal microscope (Leica SP5, Germany). For a general quantification of cell morphology ...
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bioRxiv - Neuroscience 2024Quote: The paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 31 NC subjects) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany). Hematoxylin and eosin (H&E ...
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bioRxiv - Cancer Biology 2024Quote: ... 16 formalin-fixed paraffin-embedded (FFPE) blocks from 15 patients were sectioned into 5µm slices and placed onto BOND Plus slides (Leica Biosystems, Wetzlar, Germany). Hematoxylin and eosin (H&E ...
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bioRxiv - Synthetic Biology 2024Quote: ... the fixed samples were dehydrated in a graded series of water-ethanol solutions ranging from 30% to 100% ethanol and dried by a critical point dryer (Leica EM CPD030). The top surface of the BC pellicle was coated with platinum under vacuum and observed via a scanning electron microscopy (Hitachi SU8010 ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal slices of 50 µm thickness were cut from 4.5% agarose-embedded brains using a vibrating microtome (Leica VT1000S; Leica Microsystems, Wetzlar, Germany) and hemisections were collected in 0.1M PBSA in 24-well cell culture plates (CLS3526 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 14-μm sections were prepared from the resulting blocks on a cryomicrotome (Microm HM 500 OM) and placed onto polyethylene napthalate membrane slides (Leica Microsystems, 11505158) which had been exposed to UV irradiation in a biosafety cabinet (Baker SterilGARD ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were quickly dissected out and parasagittal slices from both hemispheres (300 µm thick) were cut on a vibratome (Leica VT1200S, Germany) in the same oxygenated ice-cold cutting solution ...
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bioRxiv - Neuroscience 2024Quote: ... Two images (10X objective) from every subject within the corresponding hippocampal area were acquired under an epifluorescence microscope (DM5500B, Leica, Solms, Germany) using the LAS X Core software (Leica Microsystems ...
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bioRxiv - Neuroscience 2024Quote: In situ hybridization was performed on 16 μm thick midbrain slices from a fresh-frozen mouse brain cut on a cryostat (Leica CM3050 S). All FISH reagents used are commercially available from ACD bio ...
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bioRxiv - Neuroscience 2024Quote: ... obtained at an angle of 15° from horizontal view35 were collected in ice cold slicing ACSF on a vibrating microtome (Leica Microsystems; VT1200S). Slices were placed in a chamber for 30-35 minutes at 35° C in recovery ACSF ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... by the red fluorescence in the ocelli due to M{3xP3-RFP.attP}ZH-102D insertion when they are fathered by the male from GFP-labeled sperm lines using a fluorescent stereo microscope (Leica MZ10 F). For sperm counting experiments ...
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bioRxiv - Bioengineering 2024Quote: ... three different positions per gel were acquired at the gel center from two independent samples using a confocal microscope (Leica SP5, Germany). For a general quantification of cell morphology ...
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bioRxiv - Bioengineering 2024Quote: ... three independent positions per gel were acquired at the gel center from two independent samples using a confocal microscope (Leica SP5, Germany). For a general quantification of cell morphology ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue punches of the mPFC (containing the anterior cingulate and prelimbic cortices) were taken from 150µm coronal sections cut on a cryostat (Leica Biosystems, Wetzlar, Germany). RNA was extracted using a RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... obtained at an angle of 15° from horizontal view86 were collected in ice cold slicing ACSF on a vibrating microtome (Leica Microsystems; VT1200S). Slices were placed in a chamber for 30-35 minutes at 35° C in recovery ACSF ...
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bioRxiv - Biophysics 2024Quote: ... and coronally cut from the front to the back into 60 µm-thick sections using a cryostat microtome (Polycut CM 3500, Leica Microsystems, Germany). The brain sections were mounted on glass slides ...
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bioRxiv - Cell Biology 2024Quote: ... five subregions with minimal damage and artifacts were randomly acquired from each histological adipose tissue section using a Leica DMLB microscope (Leica, Wetzlar, Germany) coupled with a MotiCam580 (Motic ...
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bioRxiv - Genetics 2024Quote: Heart samples dissected from adult zebrafish were embedded in a tissue freezing medium and sectioned at 8 μm using a cryostat (Leica CM3050 S). Sections were air dried for 30 minutes (mins ...
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bioRxiv - Plant Biology 2020Quote: We collected the newly opened florets from the flower heads and analyzed the morphology of the florets under a light microscope (Leica Microsystem, Wetzlar, Germany) (Fig ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Neuroscience 2022Quote: ... with a thickness of 300 μm were prepared from postnatal P30-40 mice using a vibrating blade microtome (VT1200S; Leica Biosystems, Nussloch, Germany). Brains were sliced in a 95%O2 / 5%CO2-bubbled ...
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bioRxiv - Neuroscience 2020Quote: ... Confocal images of brain sections from P301L and non-transgenic littermates were obtained using a Leica SP8 confocal microscope (Leica Microsystems GmbH, Germany) at ScopeM ETH Zurich Hönggerberg core facility ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Cancer Biology 2020Quote: ... Number of adhered cells was observed under fluorescent microscope and photographs were taken from four independent fields (Leica DMI 6000B Fluorescence Microscope attached with Leica DFC 450C camera). Quantization of adhered cells was done in triplicates and each experiment was repeated thrice.
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bioRxiv - Neuroscience 2022Quote: ... The epidermal layer was manually dissected from dermal and subdermal compartments of the skin sample and transferred into a type A aluminium specimen carrier (Leica Microsystems, Wetzlar, Germany) with recesses of 200 µm containing polyvinylpyrrolidon and capped with a second carrier without recess (Leica Microsystems ...
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bioRxiv - Genetics 2022Quote: ... Light was delivered in pulses of 30s of blue LED (from the LED light source of the inverted laboratory microscope LEICA DM IL LED) at 1 Hz (Table S3) ...
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bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Neuroscience 2021Quote: ... Sagittal vermal slices and coronal floccular slices at 50μm were obtained from two animals using Leica VT1000S vibratome (Leica Biosystems, Buffalo Groves, IL). For immunolabeling of inhibitory synapses and the unipolar brush cells ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μm thick sections were cut with a glass knife from each resin-embedded tissue block on an ultramicrotome (Leica EM UC6, Leica Microsystems GmbH, Austria) and stained with 0.5 % toluidine blue in 0.5 % sodium borate ...
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bioRxiv - Microbiology 2020Quote: ... and 3x 100%) was then used to remove all water from the biofilms before they were critical point dried (Leica CPD 030, Austria).
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bioRxiv - Plant Biology 2021Quote: ... root images were taken from main roots of 12 days old seedlings with a dissection microscope (Leica M165 FC equipped with a digital Leica DFC 7000T camera). For the measurements of main root length and lateral root length ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Physiology 2022Quote: ... Images were acquired with a HC PL APO 10/0.40 CS2 objective on an inverted laser scanning confocal microscope (TCS SP8) using LASX software (all from, Leica Microsystems; Buffalo Grove, IL). A Dragonfly High Speed Confocal Microscope System [Leica DMi8 microscope coupled to an Andor Dragonfly 200 and integrated Fusion software (Oxford Instruments ...
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bioRxiv - Plant Biology 2022Quote: ... Ultrathin sections of 50 nm thick were cut transversally at 1.8mm from the root tip using a Leica Ultracut (Leica Mikrosysteme GmbH, Vienna, Austria), picked up on a copper slot grid 2 x 1 mm (EMS ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... At least 15 desmin-positive cardiomyocytes were imaged from each experiment with an inverted confocal microscope (Leica TCS SP5 AOBS, IBPS imaging platform). All images were acquired with a Z-step of 0.5 µm (image size 512x512 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections of 65 nm were cut from the outer surface of the tissue with an ultramicrotome UC7 (Leica Microsystems Inc., Buffalo Grove, IL) using a diamond knife (Diatome ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...