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Citations for Leica :
651 - 700 of 1513 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Neuroscience 2021Quote: ... Z-stack images of 1 µm interval (total depth of 4 µm) were processed with LAS software (Leica) and analyzed with Fiji software ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Cell Biology 2020Quote: ... SSCs were incubated with the labeled EVs for 1–4 h and visualized using fluorescent microscopy (Leica, Wetzlar, Germany)
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Cell Biology 2024Quote: ... Resin blocks were trimmed to approximately 2 mm2 and sectioned at 70 nm using a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc., Buffalo Grove, IL) and a Diatome diamond knife (Electron Microscopy Sciences ...
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bioRxiv - Biophysics 2021Quote: ... and incubated for 10 s prior to blotting on Whatman 1 paper for 4 s and plunge-frozen in liquid ethane using a GP2 plunge freezer (Leica) equilibrated to 10°C and 85% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Cell Biology 2022Quote: ... the pulp chambers of the mandibular first molars were opened by a high-speed handpiece with #1/4 carbide round bur under direct visualization by a stereoscopic microscope (Leica). A #10 endodontic K file was then used to probe the root canals ...
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bioRxiv - Immunology 2024Quote: ... kidneys were fixed overnight at 4°C in paraformaldehyde 4% and embedded in paraffin (Leica, Wetzlar, Germany). Sections of 5 µm were dewaxed ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature, incubated with 20% sucrose (Fisher BioReagents, BP220-1) overnight at 4°C and then embedded in OCT (Leica, 14020108926) and stored at −80°C until further use.
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C with the following dilutions in blocking buffer: p53 (CM5) (1:300, NCL-L-p53-CM5p, Leica Biosystems), Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...