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Citations for Leica :
651 - 700 of 1140 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections (20 μm) were cut around the bregma 0 to -2 mm using a Leica CM1950 cryostat (Leica Biosystems, Germany). For P2X7R immunofluorescence labelling ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Physiology 2023Quote: ... the worms were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images acquired using a confocal microscope (Leica TCS SPE) with a 63× objective lens ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Bioengineering 2023Quote: ... Lateral and longitudinal cross-sections of the samples were imaged after 7 days of culture in EGM-2 BulletKit medium using a Thunder imaging system (Leica Microsystems, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut, Leica Microsystems, Bensheim, Germany). The ultra-thin sections were post-contrasted in a LEICA EM STAIN (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the neuron somata were taken with 0.38 μm step size using a 63× oil objective with 2× digital zoom magnification (Leica TCS SPE, Germany). Stacks were then processed using FIJI/ImageJ software ...
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bioRxiv - Neuroscience 2024Quote: ... Selected sections were also studied on a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) or scanned with an Olympus VS200 Research Slide Scanner (Olympus / Evident ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+-fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software for examining Ca2+ fluctuation amplitude and frequency ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and cut into coronal slices (thickness 300 µm, -1.5 mm to 2 mm relative to bregma) on a vibratome (Leica VT1200S, Leica Biosystems, Nussloch, Germany) in ice-cooled artificial cerebrospinal fluid (aCSF) ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections (∼2 x 2 x 2cm) not needed for pathologic evaluation were fixed in 10% formalin and sliced with a vibratome (Leica VT 1200S) to a thickness of 2mm ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD3 (clone LN10, dilution 1:500, Leica), CD14 (clone SP192 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-GFAP (mouse, GA5, Leica, 1:200), anti-n-terminal APP (22c11 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour and visualised by Leica Sp8 inverted confocal microscopy in serial sections of 20 μm ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-Ki67 (1:500, Novocastra/Leica), rabbit anti-PH3 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... eMyHC (Leica NCL-MHC-d;1:200), laminin (Sigma-Aldrich L9393 ...
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 1 (Leica Cat#AR9961), Epitope Retrieval Solution 2 (Leica Cat#AR9640) ...
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bioRxiv - Cell Biology 2024Quote: ... eMyHC (Leica NCL-MHC-d; 1:200) for staining of muscle cryosections ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-p53 (1:1000; Leica Biosystems), rabbit-anti-active caspase-3 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...