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Citations for Leica :
601 - 650 of 1132 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal slices of 300 μM thick containing mPFC were obtained with a vibratome (Leica VT 1200s, Leica Microsystems, Buffalo Grove, IL, USA) in ice-cod artificial cerebral spinal fluid [aCSF containing ...
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bioRxiv - Neuroscience 2020Quote: ... Then coronal brain slices (300-320 µm thickness) containing somatosensory cortex were prepared with a vibratome(Leica VT 1200S, Leica Biosystems Inc) in a sucrose-based ice-cold solution (containing [in mM]) ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings were transferred to IAA containing liquid medium (10 µM) and imaged after 24 h (Leica TCS SP8 MP).
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bioRxiv - Neuroscience 2021Quote: ... Block of tissue containing the brainstem and cerebellum was then mounted on a cold plate of the vibroslicer (Leica VT1000S, Heidelberg, Germany) and cut into 250 μm-thick acute coronal slices ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal slices (400 μm thick) containing the entorhinal-hippocampal connections were cut approximating the horizontal plane using a vibratome (Leica VT1000 S) and transferred to an interface-type chamber and perfused with aCSF (~2.5 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... and horizontal slices containing the mid temporal hippocampus were cut at 350 μm on a Leica VT1200 (Leica Microsystems, Buffalo Grove, IL). Sections were incubated in a holding chamber kept at 34°C ...
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bioRxiv - Neuroscience 2020Quote: ... Then coronal brain slices (300-320 µm thickness) containing somatosensory cortex were prepared with a vibratome(Leica VT 1200S, Leica Biosystems Inc) in a sucrose-based ice-cold solution (containing [in mM]) ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Microbiology 2022Quote: ... and the success of the intravitreal injection was confirmed by visualizing the fluorescein-containing vitreous through the dilated pupil with a Leica M620 TTS ophthalmic surgical microscope (Leica Microsystems, Inc). Eyes with significant hemorrhage ...
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bioRxiv - Microbiology 2022Quote: ... The eye was examined and the success of the subretinal injection was confirmed by visualizing the fluorescein-containing bleb through the dilated pupil with a Leica M620 TTS ophthalmic surgical microscope (Leica Microsystems, Inc). Eyes with significant hemorrhage or leakage of vector solution from the subretinal space into the vitreous were excluded from the study ...
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Neuroscience 2024Quote: ... Tilted at 30° horizontal cortico-striatal slices or horizontal slices containing SNpc were prepared on a vibratome (Leica VT 1200S, Leica Biosystems) in ice-cold choline chloride-based cutting solution of the following composition (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal slices containing DCN were cut at 210 µm in ice-cold sucrose solution on a vibratome ((VT1200S; Leica Microsystems, Wetzlar, Germany) or (7000smz-2 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections from electrophysiological or calcium imaging experiments containing the vH were examined with a fluorescent microscope (M205 FCA, Leica Microsystems, Wetzlar, Germany) using a 2.0x magnification objective ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Acute brain slices containing perirhinal cortex and hippocampus were prepared at 275 μm thickness using a vibratome (Leica VT1200 S, Wetzlar, Germany). Slices were incubated for 25 min at 35° C and then transferred at room temperature into a custom made holding chamber.
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bioRxiv - Neuroscience 2023Quote: ... The brain was cut into 400 µm thick horizontal slices containing the hippocampal formation with a vibratome (Leica VT 1200S, Leica Biosystems, Germany) and slices were transferred to interface-type recording chambers perfused with warm and carbogenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2023Quote: ... Formalin-Fixed Paraffin-Embedded blocks containing tumor or matched normal patient samples were cut into sections of 4μm thickness using a microtome (Leica, HistoCore RM2125 RTS) (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Neuroscience 2024Quote: ... Three-four coronal midbrain slices containing the dorsal raphe nucleus were cut (300 μm thick) using a Leica vibratome (VT1200S, Leica Microsystems, Germany) and immediately transferred to a holding chamber filled with the normal extracellular solution (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was rapidly removed and 300 μm coronal slices containing the PVN were cut using a vibroslicer (Leica VT100S, Leica Biosystems, Germany). Slices were then allowed to recover for at least 1 h at 30°C in a holding chamber filled with oxygenated (95 % O2 / 5 % CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections containing wither the mHB or IPN (270 - 300 µm) were cut using a VT–1000S vibratome (Leica Microsystems, Bannockburn, IL) in ice-cold ssACSF ...
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bioRxiv - Neuroscience 2024Quote: ... brains were washed with 0.1 M PBS and free-floating 30 µm coronal sections containing BLAa were cut on a vibratome (Leica VT 1200S, Leica Biosystems, USA). Sections were stored in anti-freeze solution (30% ethylene glycol ...
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bioRxiv - Neuroscience 2024Quote: ... coronal brain slices (400 µm) containing the NAc core were collected using a vibrating tissue slicer (Leica VT1200S, Leica Biosystems, Wetzler, Germany), transferred to a recording chamber ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue punches of the mPFC (containing the anterior cingulate and prelimbic cortices) were taken from 150µm coronal sections cut on a cryostat (Leica Biosystems, Wetzlar, Germany). RNA was extracted using a RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was incubated with primary antibody against mouse p53 (CM5, Leica) overnight followed by two hours of conjugation to Protein A+G conjugated magnetic beads ...
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bioRxiv - Neuroscience 2020Quote: ... diluted in Bond Primary Antibody Diluent (AR9352, Leica Biosystems, Wetzler, Germany), for 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Anti-p53 rabbit polyclonal antibody (clone CM5) was purchased from Leica Biosystem (NCL-L-p53-CM5p ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-p53 rabbit polyclonal antibody (Leica, cat no. P53-CM5P-L), and anti-CDK12 rabbit polyclonal antibody (Atlas ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antibody staining using Polymer Refine Detection System (Leica, DS9800) using the automated Bond-III platform ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-CD4 monoclonal antibody 4B12 (1:1000, Leica Biosystems; AB_563560), and rabbit anti-IL-17 polyclonal antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and 60 µm thick coronal slices containing the CA1 region of hippocampus were cut on an oscillating blade vibratome (Leica VT1000, Leica, Germany) and transferred to PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the cell culture insert was removed and the blocks of resin containing the embedded cells were trimmed and finally cut using an ultra-microtome (Leica Microsystems, Wetzlar, Germany). Ultra-thin sections with an average thickness of 55nm were transferred on formvar-coated copper grids and stained with lead citrate ...
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bioRxiv - Immunology 2021Quote: ... Cells were extensively washed and mounted with VECTA SHIELD (containing DAPI) mounting medium and observed using a 63x oil objective on a confocal microscope (Leica SP8, Wetzlar, Germany).
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Neuroscience 2021Quote: ... and parasagittal slices containing the vermis of the cerebellum were cut at 350 mm on a Leica VT1200 (Leica Microsystems, Buffalo Grove, IL). Sections were incubated in a holding chamber maintained at 32°C ...
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bioRxiv - Neuroscience 2020Quote: Brain samples containing the hippocampus were cut serially into 16-μm-thick coronal sections using a cryostat (Leica CM 3050; Leica, Welzlar, Germany) and mounted onto glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... A block of the left hemisphere containing the entire frontal and parietal cortices was prepared and cut in the coronal plane with a vibratome (Leica model VT 1000S) into serial 150 μm-thick sections ...
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bioRxiv - Neuroscience 2020Quote: ... Horizontal slices (400 μm) containing the thalamus were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were trimmed to remove the hippocampus and the hypothalamus ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL). For identification of DAT-specific deletion of PERK and consequential reduction of phosphorylated eIF2α (p-eIF2α ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The brains were quickly dissected and coronal midbrain slices containing the DRN (300 μm) were obtained using a vibratome (VT1200S; Leica Biosystems, Wetzlar, Germany). The brain slices recovered at 34 °C for 30 min in a chamber containing the choline-Cl-based aCSF described above ...
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was removed and coronal slices (200 μm) containing either the parabrachial nucleus (PBN) or the periaqueductal gray (PAG) were sectioned on a vibratome (Leica VT1200S, Wetzlar, Germany) in ice-cold oxygenated sucrose solution (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal brain sections (45-μm thickness) containing the amygdala and surrounding regions were cut using the cryostat microtome (CM1900, Leica Microsystems, Nussloch, Germany). Sections were treated with 3% H2O2 for 10 min and then blocked with 0.1% Triton X-100 in TBS containing 2% BSA and 2% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal slices containing the IL were cut at a thickness of 240 μm using a model VT1200S vibratome (Leica Microsystems, Buffalo Grove, IL). After resting 1 hr at 37°C in aCSF ...