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Citations for Leica :
601 - 650 of 869 citations for S Adenosyl Methionine SAMe CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Then 3 μL of concentrated hTg were dispensed to the hydrophilic surface of the grid prior to single side blotting for 2 s and plunge freezing in liquid ethane using a Leica EM GP2 plunger (Leica Microsystems) operating at 20 °C and 80% relative humidity.
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bioRxiv - Neuroscience 2020Quote: ... 20 μm-thick serial sections of lumbar spinal cord were obtained using a cryostat (CM1510 S model, Leica Microsystems, Wetzlar, Germany), attached to aminopropyltriethoxysilane (APES ...
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bioRxiv - Immunology 2022Quote: ... The kidneys derived from Cx3cr1CreER/+: R26tdTomato mice were sliced into 200 μm sections with a Leica VT1000 S vibrating blade microtome (Leica Microsystems) at speed 1.80 and frequency 7 ...
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bioRxiv - Neuroscience 2019Quote: ... The brains were then removed and postfixed in 4 °C overnight and immersed in 30% sucrose solution for 2 days before being sectioned at 50 μm thicknesses on a cryostat (CM3050 S; Leica Biosystems). Imaging was performed with a scanning microscope (BX61VS ...
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bioRxiv - Bioengineering 2019Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm and 100 ms impact duration (Impact ONE; Leica Microsystems). The skin was sutured and the animals were placed in a 37°C incubator until consciousness was regained ...
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue section was mounted in an agar mount and sliced into several 100μm slices using a microtome (Leica, VT1000 S). The slices were incubated for 3 - 4 hr at room temperature with Cyanine-3-conjugated (Cy3 ...
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bioRxiv - Neuroscience 2021Quote: ... Horizontal hippocampal slices (350 µm thick) were obtained using an oscillating tissue slicer (Microm, HM650V, Thermo Fisher Scientific, or VT1200 S, Leica). Brain slices were then transferred to a holding chamber (made in-house ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... elegans behaviors were recorded at 20 frames/s through a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and a CCD camera (1940 × 1460 pixels ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Slides were imaged using a Leica SP8 confocal microscope using Leica Acquisition Suite (LAX S) (Leica Microsystems, Buffalo Grove, IL, USA). A total of 6 regions of interest (ROIs ...
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bioRxiv - Developmental Biology 2021Quote: ... Brain slices of 350–500 µm thickness were sectioned into ice-cold aCSF by using a vibrating microtome (Leica VT1200 S), transferred to 0.45-µm Millicell-CM culture inserts (MilliporeSigma ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) under a Leica S APO microscope equipped with a transillumination base and a digital camera (model MC170HD, Leica Microsystems Germany). Slices were continuously perfused at a rate of 3-3.5 ml/min at 32-23°C with the same recording solution as with whole-cell recordings and were allowed at least 20 minutes to settle in the MEA before any recordings or pharmacology was attempted ...
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bioRxiv - Neuroscience 2021Quote: ... Serial 50 µm brain (coronal or sagittal) and spinal (transverse or sagittal) sections were performed on a vibratome (Leica VT1000 S) and mounted using a DAPI staining mounting medium (Vectashield ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal brain slices (250 µm) containing the BLA were prepared using a VT1200/S vibrating blade microtome (Leica, Buffalo Grove, IL) and then incubated for 1-hour prior to electrophysiology recordings in oxygenated ...
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bioRxiv - Neuroscience 2022Quote: ... carbogenated dissecting aCSF and 300 μm transverse slices made with a cutting speed of 100 μm/s on a Leica Vibratome (Leica, CT1200). Slices were transferred to a recovery chamber filled with carbogenated pre-warmed (35 degrees Celsius ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections (14µm thick) were collected from a distance of 2.5mm rostral to the premammillary recess using a cryostat (Leica CM 3050 S). The sections were directly mounted on Superfrost Plus slides (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... polymerized at 70°C for 24 hours and then cut to 70 nm ultra-thin sections with an Ultracut S ultramicrotome (Leica, Germany). After cutting ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 s followed by high-pressure freezing after 25 s at -180°C under 2100 bar pressure in an HPM100 machine (Leica Microsystems). After freezing ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed at room temperature overnight and cut into 50 µm coronal sections on a vibratome (Leica VT1000 S).
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bioRxiv - Neuroscience 2022Quote: ... The brains were cut into 40-,tm thick sections using a vibrating blade microtome (Leica VT1000 S, Leica Biosystems, Nussloch, Germany). The sections were washed in 0.1 M PBS (3 × 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and 300 μm transverse brain sections were prepared using a vibratome (VT1000 S Vibrating blade microtome, Leica Biosystems). Fixative was replaced with 0.2 M sodium cacodylate and 0.2 M sodium cacodylate ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal slices (400 μm) containing the dorsal hippocampus or DRN were prepared in ice-cold aCSF using a vibratome (VT1200 S, Leica Biosystems). Slices were recovered in oxygenated aCSF at room temperature (22-24°C ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... Coronal slices (280 µm) containing NOPLight expression in the arcuate nucleus (ARC) were cut with a vibration microtome (VT1200 S; Leica, Germany) under cold (4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections (40 μm) through the rostro-caudal extent of the hippocampus were generated using a vibratome (Leica VT1000 S, Germany), and processed for immunohistochemistry ...
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bioRxiv - Developmental Biology 2023Quote: ... GFP images were obtained every 2 s with a x40 oil-immersive objective (HC PL FLUOTAR 40x/1.30 OIL 340 nm, Leica Microsystems CMS) used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Bioengineering 2023Quote: The brains were extracted from the anaesthetized mice and cut into 300 μm thick horizontal slices with a fully automated vibrating blade microtome (VT1200 S, Leica, Germany) in freezing cutting solution (containing 85 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in Cryo-Gel (Cat. #:475237; Instrumedics Inc.) and sectioned along the modiolus axis using a cryostat slicer (Leica CM3050 S, Leica Biosystems), at the thickness of 20 μm.
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Neuroscience 2023Quote: ... left in 4% PFA for 24h to post-fix in a refrigerator and then embedded in blocks of 1.5% agarose gel before collecting slices at 70 micrometre thickness using a vibratome (Leica VT1000 S). Slices were then stained with DAPI for 15 min (1:1000 solution) ...
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bioRxiv - Microbiology 2023Quote: ... The grid was blotted at once for 15 s from the back side and plunged into liquid ethane using EM GP2 (Leica Microsystems) at 18 °C and 90% humidity chamber setting ...
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bioRxiv - Genetics 2024Quote: ... embedded in Spurr resin and ultrathin sections (60 nm-thick) were collected with a Leica Ultracut S (Leica Microsystems, Waltzer, Germany). Sections were stained with 2% uranyl acetate and 4% lead citrate and examined on a Phillips BioTwin CM120 electron microscope equipped with Gatan Orius and Olympus Morada digital cameras.
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Developmental Biology 2021Quote: ... Gelatine blocks were flash frozen at −80 °C in pre-cooled heptane and sectioned in 25 μm slices in a cryostat (Leica, CM3050 S). The slides dried overnight at room temperature and were mounted with mowiol and sealed with nail polish prior to confocal microscopy.
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bioRxiv - Neuroscience 2022Quote: ... The tissue was then sectioned transversally at 30 μm by means of a cryostat (Leica CM3050 S, Leica Biosystems AG, Muttenz, Switzerland) and placed on microscope slides to dry for at least one hour ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Immunofluorescent staining was carried out using 14-16 μm olfactory bulb cryosections or 50μm olfactory bulb sections with vibratome (Leica VT 1000 S) prepared from animals perfused with 4% paraformaldehyde (PFA) ...
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bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
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bioRxiv - Plant Biology 2021Quote: ... the stem segments were sectioned to 80 μm native sections using an automated vibratome (Leica VT1200 S, Leica Mycrosystems Ltd., Wetzlar, Germany). These sections were stained with a 0.025 % Toluidine Blue O and regeneration was analyzed in stems by observations using a wide field microscope at 10x magnification (Zeiss Axioscope.A1 ZENAxiocam 105).
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bioRxiv - Neuroscience 2020Quote: ... Coronal 20μm-think brain sections that contained the barrel cortex were cut and collected on-slide (wS1) using a cryostat (Leica, CM3050 S). The slides were then stored at −80°C until further processing ...
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bioRxiv - Cancer Biology 2021Quote: ... left at room temperature to solidify and agarose cubes then were removed from the plastic container and mounted for Vibratome sectioning (Leica VT1200 S) using glue (cat# 14460 ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... the tissues were embedded in Optimal Cutting Temperature compound (OCT, Sakura) and sectioned into 30-μm slices using a cryostat (Leica CM3050 S). Frozen tissues stored at −80°C ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal slices (400 μm thick) containing the entorhinal-hippocampal connections were cut approximating the horizontal plane using a vibratome (Leica VT1000 S) and transferred to an interface-type chamber and perfused with aCSF (~2.5 ml/min ...
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bioRxiv - Developmental Biology 2022Quote: ... Each embryo was mounted in the embedding media in a cryosection mold placed on dry ice and 12-μm sagittal cryosections were obtained (Leica CM3050 S).