Labshake search
Citations for Leica :
601 - 650 of 869 citations for 6 Iodo quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
-
bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
-
bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
-
bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
-
bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
-
bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
-
bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
-
bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
-
bioRxiv - Neuroscience 2019Quote: Wide-field calcium imaging of Hydra was conducted at 2 Hz using a fluorescence dissecting microscope (Leica M165) equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC) ...
-
bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
-
bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
-
bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
-
bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.
-
bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
-
bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
-
Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Retrieval was accomplished using an ethylenediaminetetraacetic acid (EDTA) buffer (Bond epitope retrieval solution 2, Leica, Concord, ON, Canada), pH9 for 20 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
-
bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...
-
bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
-
bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
-
bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265 ...
-
bioRxiv - Microbiology 2023Quote: ... was applied to glow-discharged Quantifoil R2/2 grids and vitrified with a GP2 plunge-freezing device (Leica).
-
bioRxiv - Biophysics 2023Quote: ... FFPE tissue samples were cut using a ultramicrotome in 2 πm thick sections (RM2125 RTS, Leica Biosystems, Germany).
-
bioRxiv - Plant Biology 2023Quote: Roots fixed with 2% (v/v) formaldehyde in Tris were embedded in Cryo-Gel (Leica Biosystems, ID:39475237) and cut onto a cryostat (Leica CM1950 ...
-
bioRxiv - Physiology 2024Quote: ... and Mac-2-staining were imaged using a Leica DMi8 Inverted Fluorescent Microscope (Leica Microsystems, Buffalo Grove, IL). Histological quantifications were performed using Image J software (v1.52q ...
-
bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
-
bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
-
bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
-
bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
-
bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixated overnight in 4% paraformaldehyde, followed by sectioning (coronal plane, 50 μm) with a vibratome (VT100S, Leica Biosystems, Wetzlar, Germany). The slices were collected and stored in PB for immunohistochemistry.
-
bioRxiv - Immunology 2019Quote: ... eight histological sections from central region of the each alveolar socket were stained with Picrosirius Red stain and the images of 4 histological fields a 40× objective were captured by a polarizing lens coupled to a binocular inverted microscope (Leica DM IRB/E), as previously described [55] ...
-
bioRxiv - Immunology 2019Quote: Pools of 20 larvae were collected at 3- and 4-day post infection (dpi) and imaged by using the Leica MZ16FA Fluorescence Stereo Microscope (Leica Microsystems, Wetzlar Germany) equipped with the DFC420C color camera (Leica Microsystems) ...
-
bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
-
bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
-
bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
-
bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
-
bioRxiv - Pathology 2022Quote: ... in situ hybridization was performed on 12 µm-thick sections of 4% PFA-fixed OCT mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval (HIER ...
-
bioRxiv - Bioengineering 2022Quote: ... Perfusion devices were then fixed with 4% para-formaldehyde and imaged using a 16x immersion lens on SP8 multiphoton microscope (Leica Microsystems, Morrisville, NC). Tumor volumes were quantified by imaging individual tumors post bioprinting
-
bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were then embedded in a 4% agarose/1x PBS w/v solution prior to mounting and slicing on a vibratome (Leica VT 1000 S). 40 μm or 60 μm sections containing the pPVN (−0.70 to −1.06 mm relative to Bregma) ...
-
bioRxiv - Neuroscience 2019Quote: ... brains were removed from the skull and 300μm-thick parasagittal slices (horizontal for thalamic stimulation experiments) were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Individual slices were then submerged in a recording chamber mounted on the stage of an upright BX51WI microscope (Olympus ...
-
bioRxiv - Physiology 2021Quote: ... Fluorescence emission was detected every 526 ms using a Leica SP8 DIVE multiphoton confocal fluorescence imaging system as described above (emission at 460-520 nm for Fluo-4 and at 580-640 nm for Fura Red) (Leica Microsystems, Heidelberg, Germany).
-
bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.