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Citations for Leica :
601 - 650 of 876 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the transparent 96-well cell culture plate was placed on the stage of confocal microscope (STELLARIS 5, Leica) and heated to 37°C with the Stage-top Incubator System T (Live Cell Instrument) ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then embedded in low melting point agarose (σ Type VIIA, 3% in Ames medium) and 250 µm slices were obtained using a vibratome (Leica Microsystems, VT1200S) in cold (∼4° C) ...
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bioRxiv - Neuroscience 2024Quote: Fresh-frozen brain tissue samples from the three groups (n=3) were sectioned at 10 μm thickness using a cryostat (Leica Instruments GmbH, Germany). Tissue optimization was performed with TO slides (part no ...
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bioRxiv - Neuroscience 2024Quote: ... the sucrose solution was removed and organoids were placed on OCT (at least 3 organoids on each OCT drop) and cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 20 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of GN2 or scraped cells were placed between two 3 mm diameter aluminum- or gold-coated (Type A) specimen carriers with 100 µm indentations (Leica Microsystems Inc., Austria). HT29 cells directly grown on the same type of 3 mm diameter aluminum- or gold-coated (Type A ...
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bioRxiv - Neuroscience 2021Quote: ... Spheres were allowed to grow for 5 weeks and were photographed using an inverted bright-field microscope (Leica DMIRBE). Viable spheres were recovered by centrifugation at 100g for 5min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day old Arabidopsis seedling roots expressing AtC53-mCherry were cut and high-pressure frozen (EM PACT2, Leica, Germany), prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...
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bioRxiv - Pathology 2020Quote: Frozen tissue blocks were sectioned at 5 µm thickness in a Leica CM1850 cryostat (Leica Microsystems GmbH, Wetzlar, Germany) at -20C ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Zoology 2021Quote: ... Tissue sections with 5 μm thickness were cut using a microtome (Leica RM2235, Leica Biosystems Inc., Buffalo Grove, USA), fixed on slides ...
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bioRxiv - Cancer Biology 2022Quote: ... Invading cells were imaged with a sCMOS Orca-Flash 4.0LT camera (Hamamatsu) coupled 5 to an inverted DMi8 microscope (Leica), capturing serial z-stacks every 10 µm with a 10× objective (dry ACS APO 10x/NA 0.3) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63 ×/1.40 oil objective (Leica). FAOBlue was excited at 405 nm ...
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bioRxiv - Neuroscience 2021Quote: ... bubbled with 95% O2-5% CO2 and sliced using a Vibratome (Leica VT1200, Leica Microsystems Inc, Buffalo Grove IL) except for experiments in which high Ca2+ concentrations were used ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded brains were sectioned in the coronal plane at 5 µm using a microtome (Leica RM 2265). Sections were collected at 40 μm intervals ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence images of stained myotubes were obtained in 5 different areas using a DMI 3000 B microscope (Leica, Germany) and analyzed using ImageJ 1.52 software (National institutes of Health ...
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63x/1.40 oil objective (Leica). Multichannel time-lapse images were acquired in 4 fields each with 4 averages per frame in 1-min or 1.5-min intervals ...
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bioRxiv - Immunology 2021Quote: ... Organs included in paraffin were cut to a thickness of 5 microns (Microtome Leica RM2245 of disposable metal blades) and placed in a flotation solution in a water bath and then fixed on a slide sheet ...
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bioRxiv - Plant Biology 2020Quote: ... The embedded blocks were transversely sectioned at 5 to μm thickness using a Leica RM2125RT rotary microtome (Leica Microsystems) on RNase-free polyethylene naphthalate PEN membrane slides (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Biochemistry 2023Quote: ... The paraffin-embedded tissues were sectioned (5 µm thick) with a semi-automated rotary microtome (Leica Microsystems, Wetzlar, Germany) and placed on microscope glass slides coated with poly-L-lysine ...
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bioRxiv - Zoology 2023Quote: ... Paraffin blocks were sectioned (5-7 μm, 20-40 sections per block) using a microtome (Leica RM 2125RT, Germany). Histological sections were stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Physiology 2022Quote: ... The samples were imbedded in paraffin followed by serial sectioning at 5 µM thickness using the Leica microtome (Leica). The sections were deparaffinized ...
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bioRxiv - Biophysics 2023Quote: ... They were imaged with sequential z-stacks using a similar procedure as for the 3D colonies but with an 5× air objective (Nplan5×0.12PHO, Leica). The voxel size in (x,y,z ...
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bioRxiv - Neuroscience 2024Quote: ... PL Apo 40x/0.85 CORR CS and PL APO 63x/1.40 oil CS2 and Leica Stellaris 5 confocal microscopes (Leica Microsystems) equipped with a HCPL FLUOTAR 10x/0.30 ...
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bioRxiv - Neuroscience 2024Quote: ... saturated with 95 % O2 and 5 % CO2 to obtain 250 μm-thick coronal sections with a vibratome (Leica VT1200S). Slices were transferred and incubated in a holding chamber containing (ACSF ...
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bioRxiv - Developmental Biology 2024Quote: Fixed samples were imaged in a Leica Stellaris 5 upright confocal microscope using a HC PL APO 40x/1,1 Water CORR CS2 objective (#506425, Leica). The microscope was operated by the LAS X (v4.5.0.25531 ...
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bioRxiv - Immunology 2024Quote: ... The stage-top chamber was set onto the stage of a Stellaris 5 confocal microscope (Leica Microsystems, Deerfield, IL). Transmitted (brightfield ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Plant Biology 2022Quote: ... Stained seed coats were fixed with 4-5% agarose gel and dissected by microtome (Leica, VT1000 S, Wetzlar, Germany) and were visualized by Nikon 80i-epi-microscope ...
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bioRxiv - Bioengineering 2023Quote: ... the grips were attached to a custom mechanical testing rig mounted on an upright confocal microscope (Leica STELLARIS 5) in a bath with 1× PBS (Fig ...
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...