Labshake search
Citations for Leica :
551 - 600 of 768 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Tile-scan z stack images (10-15 optical sections depending on slice thickness) were collected using an SP8 confocal microscope (Leica) with a 20X magnification lens and Leica LASX software ...
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Microbiology 2019Quote: ... Several separate positions of each cell mixture were imaged every 10-15 min after auto-focusing using the LASX software package (Leica). Images were further processed with the FIJI software using the Bio-Formats plugin [61] ...
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bioRxiv - Developmental Biology 2019Quote: ... cxcr4b:h2a-mCherry embryos with and without the hsp70l:zGrad-IRES-h2a-TagBFP transgene were heat shocked around 29-30 hpf at 39.5 °C for one hour in a water bath and imaged 80 min after the end of heat shock every 10 min for 9.5 hours on a Leica SP5 II confocal microscope equipped with HyD detectors (Leica Microsystems) using a 20x (NA 0.5 ...
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bioRxiv - Developmental Biology 2019Quote: ... heat shocked around 31 hpf at 39.5 °C for 30 min in a water bath and imaged 35 min after the end of heat shock every 10 min for 4.8 hours on a Leica SP5 II confocal microscope equipped with HyD detectors (Leica Microsystems) using a 40x (NA 0.8 ...
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bioRxiv - Genetics 2021Quote: ... Samples were frozen in an ethanol/dry-ice bath and 10 to 14-micron thick sections were cut with a cryostat (Leica). Sections were permeabilized and blocked for 1-hour at room temperature with 0.3% Triton X-100 and 10% normal donkey serum ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue was sectioned on an adhesive tape at 10-μm thickness using a CM1950 (w/Cryolane) cryostat (Leica, Wetzlar, Germany) at −30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the gap widths of scratch re-population were recorded under the microscope at 10 X magnification (Leica DMi8, Wetzlar, Germany) by measuring ten consistently sized areas per well ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Cell Biology 2022Quote: ... and skeletal muscle (10 µm thickness) were cut using a Leica CM3050 S cryostat (Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Plant Biology 2022Quote: ... roots were dipped in propidium iodide (PI) solution (10 µg/ml) for 30 secs to stain the root cell wall (Leica SP8 confocal microscope ...
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bioRxiv - Microbiology 2022Quote: ... The coverslips were fitted onto sample stubs with carbon adhesive tabs and sputter coated with 10 nm tungsten (Leica SCD500). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... organoids were cryopreserved, embedded in OCT (Cell Path Ltd, Newtown, UK) and cut (10 µm) on a cryostat (Leica Cm1860). IF was performed as previously described [59] ...
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bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.
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bioRxiv - Cancer Biology 2023Quote: ... snap-frozen livers were embedded in Tissue-Tek® O.C.T (Sakura: 4583) and cut in 10 μm sections using Leica Cryostat (Leica Biosystems). 5 sections in a distance of 100 μm to each other were stained per liver ...
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bioRxiv - Zoology 2023Quote: The histochemical investigation was conducted on tegumental enzymes using frozen sections prepared and cut to 10-12 µm in a cryostat (CM 3050S, Leica). The modified lead nitrate method detected acid phosphatase (AcPase ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Neuroscience 2023Quote: ... Images were captured at 10-s intervals for 12 minutes by using a confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cross-sectional area of central canal was normalized to the baseline area in 0 minute ...
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bioRxiv - Biochemistry 2024Quote: ... The grids were then blotted for 3.0 s at 10°C and 75% humidity and plunge-frozen in liquid ethane using an EMGP2 (Leica, Germany). Cryo-EM data were collected with a 300 kV Krios G4 transmission electron microscope (Thermo Fisher Scientific ...
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Glia detect and mount a protective response to loss of dendrite substructure integrity in C. elegansbioRxiv - Neuroscience 2023Quote: Animals were anesthetized on ice for ~10-60 minutes prior to observation with a fluorescence dissecting microscope equipped with a 2X objective (Leica). Animals with obvious GFP accumulation at the nose tip were scored as strong ...
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bioRxiv - Physiology 2024Quote: ... The number of neutrophils that migrated to the lower side of the membrane was counted in 10 random microscope fields using the x20 objective (Leica). Neutrophils that passed through the membrane were quantified ...
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bioRxiv - Neuroscience 2024Quote: ... The number of body bends per 20 seconds for at least 10 worms was recorded for worms in off-feed and on-feed conditions using stereo-zoom microscope (Leica)28 ...
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bioRxiv - Neuroscience 2024Quote: ... A segment from 12 mm to 17 mm from the lesion site was serially cut in 10 µm longitudinal sections in a cryostat (Leica) and picked up in glass slides ...
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bioRxiv - Immunology 2021Quote: ... Western blot was performed according to the supplier’s instructions using rabbit polyclonal anti-p53 antibody (CM5, Leica) and rabbit-monoclonal anti-vinculin antibody (EPR8185 ...
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bioRxiv - Microbiology 2021Quote: ... For both antibodies antigen unmasking was undertaken using Heat Induced Epitope Retrieval (HEIR) solution 1 (Leica AR9961) for 30 minutes at 100°C.
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Cancer Biology 2021Quote: ... five-micron sections of formalin-fixed paraffin-embedded tissue were stained using antibody against Androgen Receptor [(Leica AR-318-L-CE ...
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bioRxiv - Bioengineering 2022Quote: ... secondary and myofiber membranes were identified with anti-α-sarcoglycan antibody (Leica Biosystems, Wetzlar, Germany, 1:200) with an Alexa Fluor™ 568 secondary (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with the corresponding secondary antibodies and visualization systems (OptiView DAB for ALK and Leica Bond Polymer Refining Kit for ROS1 and BRAF ...
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bioRxiv - Genetics 2021Quote: ... were ruled out by a monoclonal antibody against α-synuclein (NCL-L-ASYN; Leica Biosystems, Wetzlar, Germany). The staging of AD was performed by using the ABC score according to the updated National Institute on Aging-Alzheimer’s Association guidelines (50) ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of tissue-bound primary antibody was performed using the Bond Intense R Detection System (Leica Biosystems). The stained slides were converted to digital data using the NanoZoomer Digital Pathology 2.0-HT digital slide scanner (Hamamatsu).
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Cell Biology 2020Quote: ... Randomly selected hemispheres were sliced into serial sagittal plane sections with a thickness of 10 μm using a cryo-ultramicrotome (Leica, Germany), which were then stained by immunofluorescence.
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bioRxiv - Biophysics 2019Quote: ... Cryo-preserved skin in optimal cutting temperature (OCT) compound was sectioned to a nominal thickness of 10 µm (Leica CM3050 cryostat). DQ gelatin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... The number of invading neutrophils was counted in 10 random fields of view using a fluorescence microscope (Leica SP8 Confocal microscope).
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bioRxiv - Neuroscience 2019Quote: ... mice (∼10 weeks of age) were sacrificed and coronal brain slices (300 µm thickness) were acquired with vibratome (LEICA VT 1200s) in ice-cold recovery solution (containing 93 mM NMDG ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were then rinsed with PBS five times (10 min each) and sectioned coronally at 60 μm using a Leica VT1000S vibratome (Leica, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Transverse or longitudinal serial sections of 10 μm thick were made on a Reichert-Jung 23 Biocut microtome (Leica, Bannockburn, IL) of the scaffold and spinal cord areas.
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bioRxiv - Neuroscience 2021Quote: ... All slices were embedded in 10 % gelatin and further sectioned into slices of 60 μm thickness in ice-cold PB using a vibratome VT1000S (Leica Microsystems). After sectioning ...
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bioRxiv - Neuroscience 2021Quote: ... The frozen OCT block containing the organoid was then sectioned (10 µM thick) using a cryostat (Leica, Model CM 1950, Germany). For the immunocytochemistry staining ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... Samples were then mounted on aluminum stubs with carbon tape and sputter coated with 10 nm iridium (Leica EM ACE 600, Leica Microsystems) and imaged using a FEI Versa 3D scanning electron microscope (FEI ...
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bioRxiv - Neuroscience 2022Quote: ... 80% glycerol and imaged with the Leica TCS-SP2 confocal microscope using a 10x dry objective (HC PL APO CS 10×0.4, Leica, Bensheim, Germany), a 20x dry objective (HC PL APO CS 20.0×0.70 UV) ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were removed and placed in additional 10% formalin phosphate until sectioning into 40 µM thick sections via cryostat (CM 3050s; Leica Biosystems). Brain sections were stained with cresyl violet in order to visually confirm tetrode recording sites.
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bioRxiv - Systems Biology 2022Quote: ... followed by a blocking step for endogenous peroxidase using 0.3% H2O2/methanol and fixation with 10% neutral buffered formalin (Leica Biosystems, Germany). Slides were washed in Milli-Q water and 0.05% Tween20 in 1x Tris-Buffered Saline (TBS-T) ...
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bioRxiv - Cell Biology 2020Quote: ... CH) were carbon-coated to a thickness of 10 nm using the Leica EM ACE600 high-vacuum coating device (Leica Microsystems). The pattern of a Maxtaform H15 finder grid (Science Services ...
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bioRxiv - Physiology 2020Quote: Serial cross sections (10 μm) were cut from the TA and GA muscles using a Leica CM 3050S cryostat (Leica Microsystems). Muscle sections were fixed in cold acetone for 5 minutes at −20°C ...
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bioRxiv - Cell Biology 2021Quote: ... We used the optimized step intervals along the Z-axis to capture 3D images and the deconvolution of the acquired images was performed in the Leica Application Suite-X software using the tool blind deblur with 10 iterations (Leica Microsystems).