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Citations for Leica :
551 - 569 of 569 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... Larvae were then positioned and oriented laterally to acquire two z-stacks of 30 optical sections (ΔZi, Zi-1 = 683.46μm) of the gastrointestinal tract using a 4X objective (Leica HC PL FLUOTAR 4X/0.13 DRY) at 10 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Molecular Biology 2023Quote: ... For scoring (graphs Figure 1) the percentage of iPSCs expressing the chromatin markers were scored manually under an epi- fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections at 1 µm were prepared using the resin-embedded buds using a glass knife on an EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany). Sections were mounted on glass slides ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Sections were then stained for Ly6G (clone 1A8) at a previously optimized dilution (1/60000) for 30 min at room temperature with the autostainer Bond Rx (Leica Microsystems GmbH, Wetzlar, Germany). The IHC sections were visualised with 3,3’Diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2019Quote: ... was performed by the VCU Cancer Mouse Models Core Laboratory with the Leica Bond RX auto-stainer using heat-induced epitope retrieval buffer 1 (Leica, Sodium Citrate buffer, pH 6.0). Stained slides were then imaged on the Vectra Polaris (Akoya Biosciences) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...
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bioRxiv - Molecular Biology 2023Quote: ... three z-stacks of 75 optical sections each (ΔZi, Zi-1 = 151.75μm) were acquired of the bigger liver lobe using a 20X objective (Leica HCX APO L U-V-I 0.80 W). One z-stack was in bright field and acquired at 20 msec exposures ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were mounted for imaging on a Leica SP5 (siRNA experiments) or SP8 (DLD-1 experiments and experiments to assess subcellular localization) confocal microscope with an oil immersion objective (Leica HCX PL APO lambda blue, 63X, N.A. 1.4). Z-stacks were taken every 1 µm.