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Citations for Leica :
551 - 600 of 670 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Neuroscience 2023Quote: ... Braun) to a sylgard-coated cover glass covered with bath solution (Sylgard 184, Dowcorning) under a stereoscopic microscope (M50, Leica). After clearing the viscera by suction through a glass pipette ...
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bioRxiv - Neuroscience 2023Quote: ... we sectioned half-brains at 25 µm thickness on frozen 30% sucrose solution slabs on a sliding microtome (Leica SM2000R) equipped with freezing stage ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Neuroscience 2024Quote: ... brains were rapidly removed and coronal or horizontal slices were cut in ice-cold cutting solution on a Leica VT1000 S microtome (Leica Microsystems Inc ...
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bioRxiv - Neuroscience 2024Quote: ... Brains was postfixed overnight at 4 °C in a 4 % formaldehyde solution and then embedded in 5 % agarose gel for sectioning (50-µm-thick coronal sections) with a vibratome (VT1000S, Leica).
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bioRxiv - Biophysics 2024Quote: A small portion of the isolated chromosomes was stained with DAPI and imaged in solution on a glass bottom dish with an inverted fluorescence microscope (Leica DMi8 or Leica DMI6000B ...
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bioRxiv - Biophysics 2024Quote: A small portion of the isolated chromosomes was stained with DAPI and imaged in solution on a glass bottom dish with an inverted fluorescence microscope (Leica DMi8 or Leica DMI6000B ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was rapidly dissected and cut into 250 µm thick coronal slices in the same solution on a vibratome (VT1200S, Leica). Slices were then transferred to 34°C warm artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed samples were dehydrated in a series of graded alcohol solutions and embedded in paraffin blocks using a Leica TP 1020 automatic tissue processor (Leica). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... and subjected to automated baking and deparaffinization followed by heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) at 100 °C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Horizontal sections (250 μm) containing the VTA were cut in NMDG solution using a vibratome (VT1200, Leica Microsystems, Nussloch, Germany). Slices were then incubated in NMDG solution (32°C ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was promptly placed in ice-cold oxygenated cutting solution and sectioned at 250 μm using a vibratome (VT1200S, Leica). Immediately after the sectioning ...
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bioRxiv - Microbiology 2024Quote: ... were subjected to automated heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based retrieval solution (pH 9.0, Leica Biosystems) at 100 °C for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... animals were rapidly decapitated and the brain of each animal was removed and cut parasagittally in ice-cold cutting solution on a Leica VT1000S vibratome (Leica Microsystems Inc ...
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bioRxiv - Bioengineering 2024Quote: The specimens were immersed in an external cryoprotectant solution (1-hexadecene) and gently placed into HPF sample carriers (depth: 0.2 mm, Type A, Leica 16770141). The cover (Type B ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were reconstituted in an antifade solution (0.1% p-phenylenediamine in 70% glycerol) and examined under a confocal laser scanning microscope (Leica, Stellaris) with a 63x objective ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated with one drop of 3,3’ Diaminobenzidine (DAB) chromogen solution (cat.RE7270-K, Novolink MAX DAB, Leica Microsystems, Wetzlar, Germany) for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Neuroscience 2021Quote: ... 300 µm-thick coronal mPFC slices were prepared in carbogenated ice-cold slicing solution using a vibratome (Leica VT 1200 S) and allowed to recover for 20 min at 33 °C in carbogenated high-osmolarity artificial cerebrospinal fluid (high-osmolarity aCSF ...
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bioRxiv - Neuroscience 2021Quote: ... rapidly removed the brain and sectioned it in an ice-cold carbogen-saturated cutting solution using a vibratome (VT1000S, Leica Microsystems). We then incubated coronal slices (220 µm ...
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bioRxiv - Neuroscience 2021Quote: ... Horizontal slices (250 pm) containing the intralaminar thalamic nuclei were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems) and then transferred to protective recovery solution maintained at 32-34°C for 12 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Horizontal slices (300 μm) containing the thalamus were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems). Slices were transferred to artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
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bioRxiv - Neuroscience 2022Quote: Perfused brains were cryoprotected with 30% sucrose solution (Fisher Schientific, UK) and sliced at 30 μm using a SM2010R freezing microtome (Leica, UK). The slices were stained with DAPI (HelloBio ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal slices (300□μm thick) from the hippocampus were prepared in ice-cold partial sucrose solution using a microtome (Leica VT1200S) and immediately transferred in standard aCSF containing (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... 4.5 mM MgSO4) and 400 µm coronal slices were prepared in ice-cold aCSF solution using a vibrating microtome (VT1200S, Leica, Germany). Slices were transferred to aCSF solution for 30 minutes at 35 °C and current clamp whole-cell recordings were performed using a Multiclamp 700A amplifier (Molecular Devices ...
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bioRxiv - Neuroscience 2022Quote: Perfused brains were cryoprotected with 30% sucrose solution (Fisher Schientific, UK) and sliced at 50 μm using a SM2010R freezing microtome (Leica, UK). The slices were mounted using HardSet Mounting Medium with DAPI (Vector Labs ...
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bioRxiv - Cancer Biology 2022Quote: ... and rehydration procedure were performed in the system and a 20 min treatment of epitope retrieval solution I (Leica Biosystems, AR9961) was applied ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Bioengineering 2021Quote: ... Antigen retrieval was performed by incubating the sections in Leica Bond Enzyme solution (Bond Enzyme Pre-treatment Kit, Leica Biosystems, Germany) for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was quickly removed into a beaker containing the ice-cold oxygenated NMDG-based solution before obtaining 300 µm parahorizontal slices using a vibratome (VT1200S; Leica Microsystems). Sections were immediately transferred to recover in well oxygenated NMDG-based solution at 35°C for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... Sections were first applied to a peroxide blocking step for epitope retrieval using Epitope Retrieval Solution I and II (AR9640, Leica Biosystems), followed by primary antibody incubation for 30 mins at RT (PD-L1 ...
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bioRxiv - Cell Biology 2022Quote: ... They were dehydrated in a graded series of ethanol solutions and then dried by the CO2 critical-point method using EM CPD300 (Leica Microsystems). Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems) ...
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bioRxiv - Neuroscience 2022Quote: ... cryoprotected in 0.1M phosphate buffer solution containing 30% Sucrose (w/v) and cut into 50 μm thick transversal sections with a cryostat microtome (CM3050S, Leica Biosystems). Sections were counterstained with the nuclear dye 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Neuroscience 2022Quote: Mouse brains were drop-fixed overnight in 4% PFA in 1X PBS and transferred to a 30% sucrose solution in 1X PBS before slicing (40 or 50 mm) on a sliding microtome (Leica Microsystems). The slices were permeabilized and blocked in 3% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were then subject to a series of solutions for deparaffinization and rehydration using a linear stainer (Leica Biosystems, ST4020): 3× xylene ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal slices (300 µm) were then prepared in the same carbogenated solution by cutting the brain with a vibrotome (Leica, VT1200S) and left in carbon-gassed artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...