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Citations for Leica :
551 - 600 of 1194 citations for 6 Methoxy 2 methyl 1H benz de isoquinoline 1 3 2H dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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bioRxiv - Cancer Biology 2019Quote: ... Larvae were washed 3 times with PBS 1x and livers were carefully dissected with the use of forceps on a stereomicroscope (Leica MZ 9.5). After dissection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of the whole SN of Young 1 and Old 1 were obtained by sectioning the tissue (20 μm) on a freezing microtome (Leica, CM1850UV-1-1, Germany) at −24 °C.
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bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... and Ly6G (2B Scientific BE0075-1, 1:60000, ER2 Leica). Nkp46 and FoxP3 were stained on a Dako AutostainerLink48 ...
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bioRxiv - Microbiology 2023Quote: ... TTF-1 (Leica), IBA1 (Fujifilm) ...
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bioRxiv - Molecular Biology 2019Quote: ... To increase contrast the grids were stained with 2% aqueous uranyl acetate and lead citrate (Leica). The grids were examined using a Philips CM 100 Compustage (FEI ...
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bioRxiv - Cancer Biology 2020Quote: ... shRalA and shRalB cells with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica), using micro-forged glass capillaries (25 to 30 mm inner diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were first permeabilized with heat in Bond Epitope Retrieval solution 2 (pH 9.0, Leica - AR9640) at 95°C for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100°C for 30mins ...
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bioRxiv - Microbiology 2021Quote: ... They were then freeze substituted in a EM AFS 2 system (Leica Microsystems GmbH, Wetzlar, Germany) and embedded into Epon resin ...
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bioRxiv - Neuroscience 2020Quote: ... (2) The SHG signal was collected from above the retina through a x40 0.8 NA (Leica) objective ...
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bioRxiv - Cancer Biology 2020Quote: ... At least 2× step sections per lung were stained on a Bond Automated Stainer (Leica Biosystems) using standard protocol F and Target Retrieval Solution pH 6.0 (Dako ...
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bioRxiv - Plant Biology 2020Quote: ... leaf discs were collected 2 dpi and observed using a Leica TCS SP8 (Leica, Mannheim, Germany) confocal microscope with the following excitation wavelengths ...
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bioRxiv - Microbiology 2019Quote: ... 2) the original bright-field condenser was replaced with an air objective (40× NA = 0.6, Leica); and 3 ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were mounted in 2% agarose and sectioned at 50μm using a vibratome (Leica VT1000s, Germany). Coronal sections were used for all brains ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were mounted in 2% agarose and sectioned at 50 μm using a vibratome (Leica VT1000s). Coronal sections were used for all brains ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in 2% agarose and sectioned to 50-μm coronal/sagittal sections by a vibratome (Leica). For OE sections ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections (2 μm thickness) obtained with a Leica RM2245 rotary micro-tome (Leica Biosystems) were mounted on glass slides (Superfrost Plus ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica, cat# AR9640). Both mouse monoclonal anti-Ki67 CloneMIB-1 (Dako ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
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bioRxiv - Microbiology 2019Quote: Purified RNA was stained with cyanine 3 or cyanine 5 using the ULS Fluorescent Labeling Kit for Agilent arrays from Kreatech (Leica Biosystems, Amsterdam, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Immunology 2019Quote: Pools of 20 larvae were collected at 3- and 4-day post infection (dpi) and imaged by using the Leica MZ16FA Fluorescence Stereo Microscope (Leica Microsystems, Wetzlar Germany) equipped with the DFC420C color camera (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequently the samples were immersed in 100% ethanol for 3×10 min and then critical point dried in an Automated Critical Point Dryer Leica EM CPD300 (Leica Microsystems, Vienna, Austria) with 18 steps of ethanol - CO2 exchange.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Genomics 2021Quote: ... AT2 cell purity was determined by NKX2-1 cytospin staining (1:100, Leica Biosystems, Cat # NCL-1-TTF1). Donor1 was 83% positive ...
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bioRxiv - Cancer Biology 2022Quote: ... Ly6G (clone 1A8, 2B Scientific BE0075-1, ER2 Leica, 1:60000), LTF (Thermofisher ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...