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Citations for Leica :
551 - 600 of 677 citations for 6 Isoquinolinamine 5 propyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
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bioRxiv - Neuroscience 2019Quote: ... Representative images of further experiments investigating neurite outgrowth in differentiated CTX0E16 cells were acquired using a Leica SP-5 confocal microscope with a 100x oil-immersion objective (Leica microsystems). Z-stacks of 5 individual cells per condition across three biological replicates were obtained for statistical data analysis ...
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: All experiments were imaged using a Leica SP-5 confocal microscope using a Plan-Apochromatic 63x 1.40 NA oil-immersion objective (Leica microsystems, 506210). Fluorophores were excited using a 100 mW Ar laser (458 ...
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bioRxiv - Plant Biology 2019Quote: ... The root segments were embedded in agarose 5% and cut into 25 μm radial sections with a Leica VT1200S Leica vibratome (Leica Microsystems). Sections were categorized according to their distance (100 ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...
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bioRxiv - Neuroscience 2022Quote: ... Their brains were quickly dissected out and stuck on a 5 mm agar stage before being mounted on a vibratome (Leica VT1200S) equipped with a sapphire blade ...
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bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... the lumbar spinal cord from L3-5 was mounted on an agarose mold and the dorsal halve was micro dissected on a vibratome (Leica Microsystems). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... LEGE 07298 and Phormidesmis priestleyi ULC 007 stained with either calcein or 5-CF were acquired with an inverted confocal laser scanning microscope (CLSM; Leica SP8) using a HC PL APO x63/1.40 oil objective ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Confocal images were captured at 63X oil immersion objective on Leica STELLARIS 5 (Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X software ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were imaged with a laser scanning confocal Stellaris 5 or TCS SP8 gSTED equipped with a white light laser and a 405-diode ultraviolet laser (Leica Microscope). Image acquisition was done using the LAS-X software in accordance with the Nyquist sampling sequential mode by exciting the fluorophores and collecting their signal with hybrid detectors (HyDs).
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Cell Biology 2023Quote: ... All confocal images were acquired using the Leica TCS SP8 or Stellaris 5 confocal microscope with the Leica LAS X software (Leica Microsystems). Following objectives were used with the Leica TCS SP8 ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2023Quote: Lengths of root RAM and EZ regions and root hairs in 5-d-old seedlings grown on vertical MS medium without sucrose were measured under a Leica microscope (Leica DM5000). The data were analyzed using the ImageJ software (http://rsb.info ...
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Plant Biology 2024Quote: ... mounted in H2O on a slide and imaged with a 63x glycerol immersion objective on a Leica TCS SP8 microscope or a Leica Stellaris 5 microscope (Leica Microsystems). On the SP8 we imaged using an Argon laser at 20% power ...
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bioRxiv - Biophysics 2024Quote: ... we deposited approximately 5 nm of carbon to the nanopipettes using thermal evaporation (CED 030, BalTec) with double-stranded carbon thread (Leica Microsystems). We imaged the nanopipettes by scanning electron microscopy (LEO 1530 VP ...
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bioRxiv - Developmental Biology 2024Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L2-5) was sectioned transversely (300-350 µm) with dorsal roots attached using a vibrating microtome (Leica Microsystems). Slices were next transferred to an artificial cerebrospinal fluid (ACSF) ...
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bioRxiv - Biophysics 2024Quote: ... The removed brain was washed thrice with PBS (5 min each, shaking in a shaker) and subsequently sectioned into 100 µm thick sections using a vibratome (Leica, VT1000S). The resulting brain sections were subjected to label-free CARS and SHG imaging.
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5 – 7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Developmental Biology 2020Quote: Pregnant female mice (E3.5 and E9.5) were sacrificed by cervical dislocation for embryo dissections under a dissecting microscope (M165C or M80, Leica Microsystems; Wetzlar, Germany) as previously described (Behringer et al ...
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bioRxiv - Immunology 2021Quote: ... Fixed tissue samples were then embedded in paraffin and 5 µm thick sections were cut at a rotary microtome (Leica, Wetzlar, Germany). After a 10 min incubation in PBS ...
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bioRxiv - Neuroscience 2019Quote: Confocal images of double-stained neurons were acquired with a Leica SP-5 confocal microscope using a 63x oil-immersion objective (Leica, N.A. 1.4) as a z-series ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: we measured the teleochrysalid abdomen surface area (Troscianko, 2014) from high-resolution photos (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Cancer Biology 2019Quote: ... a minimum of 5 images were taken of cells per experiment using a 20x water objective on an inverted confocal microscope (Leica TCS SP8). Using phalloidin staining and Image J software ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...