Labshake search
Citations for Leica :
551 - 600 of 1409 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections ...
-
bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
-
bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
-
bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
-
bioRxiv - Neuroscience 2022Quote: ... Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v) sucrose for two days and then embedded in tissue freezing medium (Leica Microsystems ...
-
bioRxiv - Plant Biology 2022Quote: ... The samples were then coated with 3 nm platinum and 4 nm carbon using the ACE600 coating device (Leica Microsystems).
-
bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
-
bioRxiv - Neuroscience 2024Quote: ... The brains were kept in 4% PFA at least overnight and stored in PBS until histological slicing (Leica VT1200 S). DAPI-Fluoromount-G (70-100 µm slices ...
-
bioRxiv - Neuroscience 2024Quote: ... electroporated cerebella were embedded in 4% LMP agarose dissolved in mHBSS and then cut into 300 mm sagittal slices using a VT1200 vibratome (Leica). The slices were finally placed on 0.4 mm PTFE cell culture inserts (Millicell ...
-
bioRxiv - Genomics 2024Quote: ... Sections of 4 μm without wax were stained with safranin and fast green and photographed with electric inverted fluorescence microscope (MDi8, Leica).
-
bioRxiv - Neuroscience 2024Quote: ... Stained sections were stored at 4°C and imaged using the Inverted Leica DMIL LED Microscope with the Thunder imaging software (Leica).
-
bioRxiv - Developmental Biology 2023Quote: ... Undecalcified frozen sections at a thickness of 4 µm were obtained according to Kawamoto’s film method using TC-65 (Leica Microsystems) or SL-T35 (UF ...
-
Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
-
bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
-
bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
-
bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
-
bioRxiv - Bioengineering 2023Quote: ... The brains were extracted and postfixed for 24-48 hours in the same fixative at 4°C before being sliced into 50 μm coronal sections using a vibratome (Leica). The slices were blocked in 10% normal goat serum (SouthernBiotech ...
-
bioRxiv - Neuroscience 2022Quote: ... The paraffin embedded sections were then sliced into 4 μm-thick slices on a Leica RM2016 Microtome (Leica, Shanghai, China) for immunohistochemistry staining and apoptotic cells labeling ...
-
bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 4% agarose before being cut into 40 um coronal slices with a vibratome (Leica VT S10000) and stored in 0.1% PBS Sodium Azide ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were then coated with a 4 nm layer of platinum using a ACE600 high resolution sputter coater (Leica Microsystems) before being imaged using an FEI Quanta 450 ESEM (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
-
bioRxiv - Molecular Biology 2023Quote: ... Immunohistochemistry or immunofluorescence experiments were performed on 4 µm-sections using Novolink polymer detection system (7140-K, Leica Micro-systems) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
-
THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA overnight and then sliced into 100 um coronal sections using a vibratome (VT1200s, Leica). Brain slices were imaged by a fluorescence microscope (BX63 ...
-
bioRxiv - Bioengineering 2024Quote: ... and finally coverslips were mounted on SEM stubs with carbon tape and metallized with 4 nm of platinum (Leica ACE600). Electron microscopy was carried out using a field emission gun scanning electron microscope (SEM ...
-
bioRxiv - Bioengineering 2024Quote: ... at 4°C and embedded in OCT for generation of 15 µm coronal sections using a CM3050 S cryostat (Leica).
-
bioRxiv - Neuroscience 2024Quote: ... containing 4 μM QSY21 to quench the extracellular QD signal by using a confocal laser scanning microscope (TCS SP8, Leica) at a 1 Hz rate ...
-
bioRxiv - Neuroscience 2024Quote: Perfused brains (4% paraformaldehyde) were sectioned at 50-60 µm using a vibratome (Leica VT1000, Leica Microsystems, Nussloch GmbH, Germany). Sections were washed twice for 5 minutes each in phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2024Quote: Mice brains were harvested after perfusion with 4% PFA in PBS1X and post-fixed in 4% PFA for 2 hours on ice or at 4°C.Brains were then washed with 1XPBS three times and cut in cold PBS1X using a vibratome (Leica VT1200S), in 40 µm thick sections.Brain sections were mounted onto positively charged microscope slides (Superfrost PlusTM Microscope Slides White Tab ...
-
bioRxiv - Cell Biology 2024Quote: Mice lung specimens were fixed in 4% paraformaldehyde for 48 h and subsequently embedded in paraffin using ASP200S and EG1150H (Leica). At the time of death ...
-
bioRxiv - Cell Biology 2024Quote: ... kidneys were embedded in low-melting agarose (4%) and cut into 300 µm slices using a vibratome (VT1200 S, Leica). Slices were maintained in Krebs-Henseleit-Buffer carbonated with 95% O2/5% CO2.[24] For imaging ...
-
bioRxiv - Cancer Biology 2020Quote: ... shRalA and shRalB cells with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica), using micro-forged glass capillaries (25 to 30 mm inner diameter ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were first permeabilized with heat in Bond Epitope Retrieval solution 2 (pH 9.0, Leica - AR9640) at 95°C for 2 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100°C for 30mins ...
-
bioRxiv - Microbiology 2021Quote: ... They were then freeze substituted in a EM AFS 2 system (Leica Microsystems GmbH, Wetzlar, Germany) and embedded into Epon resin ...
-
bioRxiv - Neuroscience 2020Quote: ... (2) The SHG signal was collected from above the retina through a x40 0.8 NA (Leica) objective ...
-
bioRxiv - Cancer Biology 2020Quote: ... At least 2× step sections per lung were stained on a Bond Automated Stainer (Leica Biosystems) using standard protocol F and Target Retrieval Solution pH 6.0 (Dako ...
-
bioRxiv - Plant Biology 2020Quote: ... leaf discs were collected 2 dpi and observed using a Leica TCS SP8 (Leica, Mannheim, Germany) confocal microscope with the following excitation wavelengths ...
-
bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were mounted in 2% agarose and sectioned at 50μm using a vibratome (Leica VT1000s, Germany). Coronal sections were used for all brains ...
-
bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were mounted in 2% agarose and sectioned at 50 μm using a vibratome (Leica VT1000s). Coronal sections were used for all brains ...
-
bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in 2% agarose and sectioned to 50-μm coronal/sagittal sections by a vibratome (Leica). For OE sections ...
-
bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections (2 μm thickness) obtained with a Leica RM2245 rotary micro-tome (Leica Biosystems) were mounted on glass slides (Superfrost Plus ...