Labshake search
Citations for Leica :
551 - 600 of 721 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 5 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Microbiology 2019Quote: ... at pH 2.5 to quantify mucous cells in the gills per inter-lamellar unit (ILU) under a bright field light microscope (Leica DM1000, Hamburg, Germany) (29 ...
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bioRxiv - Immunology 2022Quote: ... was performed on sections (5 mm thick) of formalin-fixed lung mounted onto charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
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bioRxiv - Bioengineering 2022Quote: ... 3.033 μm and z: 5 μm) of the intervention hemisphere were acquired using a confocal laser scanning microscope SP8 (Leica Microsystems, Wetzlar, Germany) with a 10x objective ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Plant Biology 2021Quote: ... The root segments were embedded in agarose 5% (w/v) and cut into 25-30 μm radial sections with a VT1200S vibratome (Leica Microsystems, Nanterre, France). Radial sections were sampled from 15 ectomycorrhizal rootlets (at 200 µm from the root apex ...
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bioRxiv - Microbiology 2022Quote: ... tremulum culture was stained with Auramine O and examined by using the Leica TCS SP 5 Confocal Laser Scanning Microscope (Leica Microsystems, Wetzlar, Germany) in the Laboratory of Bioimaging facility NCCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were examined with an Olympus BX43 light microscope and microphotographs taken using a 5 Megapixel HD Microscope Camera (Leica MC170 HD, Germany).
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bioRxiv - Cell Biology 2021Quote: The epithelium at ALI were incubated at 37°C and 5% CO2 in the 3i Marianis/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA) and high speed time-lapse videos were taken at 32X air at 100 Hz with a total of 250 frames using a scientific Hamamatsu C1140-42U30 CMOS camera (Hamamatsu Photonics ...
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bioRxiv - Cancer Biology 2021Quote: Sections with a thickness of 5 µm of lung adenocarcinoma and matched adjacent non-tumor lung tissue were mounted on MembraneSlides (Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2020Quote: ... and the brain was cut into 400-um thick coronal sections using a vibratome (Leica VT1000S; Leica, speed 5, vibration frequency 7-8), while in bubbling ice cold ACSF (recipe as above ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000; Leica Microsystems, Heidelberg, Germany) at high magnification (x200 or x400 depending on the immunolabeled density of cells) ...
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bioRxiv - Zoology 2022Quote: ... and sectioned serially into 5 μm transverse or coronal tissue sections using the Leica X Multicut semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). Additional samples from juvenile T ...
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bioRxiv - Plant Biology 2022Quote: Sections of 5 or 10 μm were prepared on a Leica JUNG RM2055 microtome using a microtome knife (Leica Disposable blades TC-65). The sections were imaged without staining or after staining with Safranin O (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Pathology 2023Quote: ... The tissue samples were decalcified and embedded in paraffin before the preparation of coronal sections of 5-μm thickness around the ligation site using a microtome (RM2245, Leica Microsystems, Wetzlar, Germany). Tissue sections were then subjected to TRAP staining using an acid phosphatase leukocyte kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
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bioRxiv - Physiology 2024Quote: ... H-1000) and visualized using a Leica Stellaris 5 Confocal Microscope equipped with a 40x oil-immersion objective (Leica Microsystems Inc, Illinois, USA). Fluorescence intensity profiles across sarcomeres were plotted offline using the FIJI software.
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bioRxiv - Evolutionary Biology 2023Quote: Rehydrated brains were embedded in agarose gel (5% UltraPure agarose power in deionised water) and sectioned using a vibrating blade microtome with an 80 µm interval (Leica VT1200, Wetzlar, Germany). Sections were then washed six times in PBS-TX (0.5% Triton X-100 detergent in phosphate-buffered saline) ...
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bioRxiv - Developmental Biology 2021Quote: ... Heart tissues fixed in 10% formalin and paraffin-embedded were sliced into serial 5-µm sections using a Leica RM2125 RTS microtome (Leica Camera AG, Wetzlar, Germany). Sections on slides were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2022Quote: ... The plates containing the pseudostratified epithelia were incubated at 37 °C with 5% CO2 in the 3i Marianis/Yokogawa Spinning-Disk Confocal microscope (Leica Microsystems, Sugar land, USA) as reported in our previous publications (53 ...
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bioRxiv - Cell Biology 2022Quote: ... Paraffin-embedded WAT was sliced into 5 μm sections using a Thermo Scientific™ HM 355S Automatic Microtome and mounted on Surgipath® X-tra® Microscope Slides (Leica Biosystems). To represent the whole tissue ...
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated with 95 % O2-5% CO2) and parasagittal spinal cord slices (280 μm) were prepared with a vibratome (VT1200S, Leica Microsystems AG, Wetzlar, Germany) in the same solution ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in ice-cold 95% O2/5% CO2-equilibrated sACSF and sectioned at 250 µm in the sagittal plane with a vibratome (VT1200S; Leica Microsystems Inc., IL, USA). Slices were then transferred to a holding chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC staining for CAV1 was performed on formalin-fixed paraffin-embedded HER2-expressing gastric tumor sections (5 μm thickness) on a Leica Bond RX automated stainer (Leica Biosystems, Buffalo Grove, IL). Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0 ...
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bioRxiv - Plant Biology 2022Quote: To generate the training set we used two different stereo microscopes equipped with an integrated camera for image capture (ZEISS Stemi 305 with a 1.2 Megapixel integrated colour camera; Leica MZ16 coupled with a Leica CLS100x white light source and a Leica DFC420 5 Megapixel colour camera). For the downstream experiments using the adapted CNNs we only used the ZEISS Stemi 305 since it is easier to operate and to transport to the field ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... The images were captured by the Leica STELLARIS 5 confocal microscope with 63X oil immersion objective ((Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X.
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3mm round tip at a speed of 3m/s ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The number of fluorescent and non-fluorescent worms was counted in triplicates in droplets of 5-20 µl depending on worm density using a fluorescent dissecting scope (Leica Microsystems GmbH, Wetzlar, Germany). Competitive fitness was determined as relative proportion of non-fluorescent mutant worms compared to the fluorescent reference strain CL2122.
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bioRxiv - Immunology 2022Quote: ... The antibody was detected using Bond Polymer Refine Detection kit (Leica Biosystems) with diaminobenzidine (DAB ...
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bioRxiv - Physiology 2020Quote: ... and staining using the BondTM Polymer Refine Detection kit (DS9800, Leica Biosystems). Brightfield images were acquired using a Leica DM 1000 LED microscope and processed using Leica Application Suite software (Leica Biosystems).
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bioRxiv - Cancer Biology 2020Quote: ... before peroxidase block was performed using an Intense R kit (Leica, DS9263). Caspase 3 was stained at a previously optimised dilution of 1/500 and Ki67 at 1/100 before washing with Leica wash buffer and then application of rabbit envision secondary (Agligent ...
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bioRxiv - Immunology 2022Quote: ... Slides were counterstained with hematoxylin (Bond Polymer Refine Detection Kit, DS9800, Leica Biosystems.
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bioRxiv - Cancer Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... with the Bond Polymer Refine Kit (cat. DS9800 Leica Biosystem, Richmond, IL). Immunostaining was performed using the Bond RX automated system (Leica Biosystems ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... the slides were stained with the Bond Refine Detection Kit (Leica Biosystems), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... For the IHC portion a Bond Refine staining kit (Leica Biosystems, DS9800) was used with a standard protocol minus the peroxide blocking step which was deleted ...
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bioRxiv - Cell Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). FFPE sections were rinsed with wash buffer and Podxl antibody applied for 30 minutes (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... and detected with DAB using the HRP Polymer Refine Detection Kit (Leica) and hematoxylin counterstaining ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed using the Bond Polymer Refine Kit (DS9800, Leica Biosystems). For dual staining ...
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bioRxiv - Cancer Biology 2024Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 min ...