Labshake search
Citations for Leica :
501 - 550 of 700 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Systems Biology 2023Quote: Frozen serial 8 μm sections of mice heart tissues were cut at −20°C with a Microtome (CM1950; Leica, Nussloch, Germany) and mounted on ITO-coated glass slides for MALDI-MSI and on coated glass slides for HE staining ...
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 40 µm in the coronal plane with a Leica SM 2000R freezing microtome (Leica Microsystems). The sections were stored at -20 °C in 24-well tissue culture plates filled with antifreeze solution (30% ethylene glycol ...
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bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Immunology 2022Quote: ... the tissue slides were heat pre-treated using a ER1 (pH6) target retrieval buffer for 15 minutes at 97°C (AR9961, Leica Biosystems). The slides were then stained for multiplex immunofluorescence using the BOND RX Opal multiplex procedure (v7.0.3.216 RX ...
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bioRxiv - Microbiology 2022Quote: ... Cryo-sections (70 nm thick) of the frozen material were obtained at −120°C using an Ultracut cryo-ultramicrotome (Leica Microsystems). The cryo-sections were collected on formvar-coated nickel grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and embedded tissue was frozen rapidly and either stored at − 80 °C or immediately sectioned into 20-μm thick frozen sections using a cryostat (Leica CM3050S). Histological sections were placed in Superfrost® slides and placed at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... thin coronal midbrain cryosections (12 µm) were cut at -20°C and transferred to RNaseZAP-treated PPS FrameSlides (Leica Microsystems, # 11600294). Six sections per FrameSlide ...
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bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
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bioRxiv - Pathology 2023Quote: Carriers containing high pressure frozen samples for cryoFIB-SEM liftout were transferred to a cryo-ultramicrotome (Leica EM FC7, −160°C) equipped with a Trim 45 T1865 diamond knife ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Cell Biology 2023Quote: The frozen blocks were removed from the-80°C freezer and allowed to sit in the cryostat (Leica CM 1950, #047742456) at-20°C for 1 hour to equilibrate ...
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Molecular Biology 2023Quote: Adult flies containing P[GMR-Gal4] and four copies of P[UAS-arm*] or its variants were frozen at −20°C overnight and photographed with a Leica Stereo Dissecting Scope (Leica DMI6000B) attached to a digital camera ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Biophysics 2024Quote: ... Mock-infected and infected MDM were vitrified by plunge freezing into liquid ethane at –183°C using an EM GP2 plunger (Leica Microsystems). The blotting chamber was maintained at 37°C temperature and 90% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue blocks containing SCEM were sectioned at −16°C into 8-micrometer slices using a CM 3050 cryostat (Leica, Wetzlar, Germany). The slices were then transferred onto indium tin oxide-coated glass slides (Matsunami Glass Industries ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed in 4% PFA for 48 h at 4°C and cut into 100 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Cell Biology 2024Quote: ... The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4) at -80°C for 72 hours in an AFS2 machine (Leica Microsystems). After being slowly warmed to RT over 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA at 4 °C and then rinsed in PBS before being sectioned at a thickness of 60 µm using a vibratome (Leica Biosystems). These sections were then mounted onto Superfrost Plus slides (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... the vitrified pellets were freeze-substituted at -90°C for 80 h in acetone supplemented with 2 % OsO4 (AFS2; Leica Microsystems). The samples temperature was increased slowly to -60°C (2°C⋅h-1) ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal slices containing the mPFC (350 μm thickness) were cut at 2 °C using a Vibratome 1000 Plus (Leica Microsystems, USA) in a NMDG-containing cutting solution (in mM) ...
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bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Plant Biology 2024Quote: ... v/v) diluted with 100% acetone at 58°C for 20 minutes in each solution followed by a graded paraffin (Leica, USA) series (50 ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were continuously illuminated for 30 s with a laser (470 nm, ∼20 mW/mm²) followed by HPF at −180 °C under 2100 bar pressure in an HPM100 (Leica Microsystems). Frozen specimens were transferred under liquid nitrogen into a Reichert AFS machine (Leica Microsystems ...
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bioRxiv - Pathology 2024Quote: ... applied at a dilution of 1:50 for 30 minutes at 25 °C in an automated Leica BOND III system (Leica Biosystems). Visualization of the primary antibody binding to tissue section was performed using BOND Polymer Refine Detection (DS9800 ...
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bioRxiv - Neuroscience 2024Quote: ... and LHA (for recordings) were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2024Quote: ... coronal sections (60-μm-thick sections) were prepared at −25°C from the brain tissue blocks using a Cryostat (Leica CM3050S). Sections were stored in 24 well plates according to their position in the anteroposterior axis of the brain at 4°C in PBS-Azide (0.01% ...
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bioRxiv - Neuroscience 2024Quote: ... Eyecups were flash frozen in this OCT/sucrose mixture and stored at -80°C prior to sectioning with a Leica CM3050S cryostat (Leica Microsystems). Sections were hydrated by washing three times with PBS before incubating in LAB buffer (PolySciences Ltd ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000, Ted Pella, or VT1000P, Leica). Sections were collected into cryoprotectant (25% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature, incubated with 20% sucrose (Fisher BioReagents, BP220-1) overnight at 4°C and then embedded in OCT (Leica, 14020108926) and stored at −80°C until further use.
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bioRxiv - Cancer Biology 2024Quote: ... and baked in a 60°C oven before staining with haematoxylin and eosin (H&E) on the Leica Autostainer X (Leica Biosystems). Immunohistochemistry (IHC ...
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bioRxiv - Cancer Biology 2024Quote: ... FFPE slides were deparaffinized and subjected to heat-induced epitope retrieval at 97 ° or 100 °C using BOND epitope retrieval solutions ER1 or ER2 (Leica Biosystems). The tissue sections were incubated with a blocking solution for 10 minutes ...