Labshake search
Citations for Leica :
501 - 550 of 1228 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections of the IC (200 μm) were cut in 34°C ACSF with a vibrating microtome (VT1200S, Leica Biosystems) and incubated at 34°C for 30 min in ACSF bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Neuroscience 2022Quote: ... Post-fixed brains were sliced in 35 μm thick sections in a -18°C chamber using a 3050 S Cryostat (Leica). Brain slices were either placed directly on Superfrost Plus slides (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal brain slices were taken at 40 µm using a cryostat at −20°C (Leica Biosystems Inc, Buffalo Grove, IL). The whole brain was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... by deparaffinization and heat-induced epitope retrieval (HIER) at 100°C for 15 minutes using ER1 epitope retrieval buffer (Leica Biosystems product ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Microbiology 2023Quote: ... The samples were incubated overnight at 28°C and analysed with a Leica HCS LSI Macro Confocal (Leica Microsystems, Germany), using a 5x objective and GFP excitation/detection settings ...
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen eyecups were sectioned to 40 μM thickness at -20°C on a Leica CM3050 S Cryostat (Leica, Wetzlar, Germany) onto TruBond 380 adhesive slides (Electron Microscopy Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was snap frozen in OCT and stored at −70°C until sectioning in to 12 µm slices on a cryostat (Leica). Thaw-mounted sections were stored at −70°C ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
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bioRxiv - Microbiology 2023Quote: Chemically fixed EBOV-infected or transfected Huh7 cells on EM grids were vitrified by plunge-freezing into liquid ethane at −183 °C using a GP2 plunge-freezer (Leica) with a chamber temperature of 25 °C and 85% humidity ...
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bioRxiv - Microbiology 2023Quote: ... solution (0.1% uranyl acetate in anhydrous acetone) the samples were cooled to −90 °C and processed in an automated FS system (EM AFS2, Leica). After a washing step in acetone ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μm slices were made of the entire dorsal hippocampus (dHPC) at -20 °C using a cryostat (CM1510 Leica Biosystems). Sections were stored individually in 96-well plates containing antifreeze solution (30% ethylene glycol ...
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bioRxiv - Biophysics 2023Quote: ... The nuclear envelope-positive area was assessed by thresholding the maximum intensity projection images of lamin A/C staining obtained by confocal microscopy (63x objective NA 1.25, Leica SP5) and merging them with nuclei masks ...
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bioRxiv - Bioengineering 2023Quote: ... The brains were extracted and postfixed for 24-48 hours in the same fixative at 4°C before being sliced into 50 μm coronal sections using a vibratome (Leica). The slices were blocked in 10% normal goat serum (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C) before being plunged into liquid ethane using an EM GP2 automatic plunge freezer (Leica). The cryo-EM data sets of NbE-µOR-Fab module complex were acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were stored at 4°C and imaged using the Inverted Leica DMIL LED Microscope with the Thunder imaging software (Leica).
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bioRxiv - Plant Biology 2019Quote: ... Transverse stem sections (10 μm thick) were obtained using a microtome (RM2125, Leica). Sections were mounted on slides coated with 50 mg/ml poly-d-Lys (Sigma Chemical Co. ...
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bioRxiv - Neuroscience 2019Quote: ... and then sectioned at 10 or 20 µm using a cryostat (CM1850, Leica). The sections were permeabilized and blocked for 1 h at room temperature in buffer G (0.1 or 1.0% Triton X-100 ...
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bioRxiv - Plant Biology 2020Quote: ... Thin sections of 10 μm were produced using a microtome (RM2165, Leica Microsystems) and counterstained for 30 min with 0.1% ruthenium red and rinsed two times with ultrapure water ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal cryosections were cut at 10 μm on a cryostat (Leica CM 1850) and mounted on gelatin precoated slides ...
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Spatially-targeted proteomics of the host-pathogen interface during staphylococcal abscess formationbioRxiv - Microbiology 2020Quote: ... Tissues were then cryosectioned at 10 μm thickness (Leica Microsystems, Buffalo Grove, IL) and thaw mounted onto custom fiducial microscope slides (Delta Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... ~10 μm-thick sections were collected on a cryostat (Leica CM3050S, Wetzlar, Germany). Sections for MALDI-MSI were thaw-mounted on indium tin oxide (ITO)-coated glass slides (Bruker Daltonics ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 10 dips in Alcoholic Eosin Y 515 (Leica Biosystems, cat. 3801615). Dehydration was performed through an alcohol gradient of 95% ...
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bioRxiv - Cell Biology 2019Quote: ... samples were subsequently sectioned at 10 µm using a cryostat (Leica, Lumberton, NJ). Sections were immersed in normal donkey serum 1:20 in PBS containing 0.5% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... for 10 min using an automatic contrasting system (Leica EM AC20, Leica Microsystems). Imaging was performed using a CM100 transmission electron microscope (Phillips/FEI ...
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bioRxiv - Neuroscience 2019Quote: ... for 10 min using an automatic contrasting system (Leica EM AC20, Leica Microsystems). Imaging was performed using a CM100 transmission electron microscope (Phillips/FEI ...
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bioRxiv - Neuroscience 2021Quote: Perfused brains were sliced at 10 µm on a cryostat (Leica CM2050 S) and immediately fused to 18 mm glass coverslips (#1.5 ...
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bioRxiv - Immunology 2021Quote: ... 7-10 μm thick sections were prepared with a cryostat (Leica CM 1950). For the H&E staining ...
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bioRxiv - Immunology 2020Quote: ... fourth inguinal mammary glands were fixed in 10% neutral buffered formalin (NBF) (Leica) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Frozen samples were cut into 10 µm sections using a cryostat (CM1850; Leica), placed onto microscope slides ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were cut into 10 µm sections using a microtome (RM2125 RTS; Leica), placed onto microscope slides (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2022Quote: ... Frozen sections (10 μm) obtained with a Leica cryostat (Leica, Buffalo Grove, IL) were placed on glass slides ...
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bioRxiv - Neuroscience 2022Quote: ... Sections (8–10 μm thickness) were prepared using a CM3050S cryostat (Leica Microsystems) and applied on precoated microscope slides (Superfrost plus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serial sections were obtained at 10 µm thick using a cryostat (Leica CM1950). Sections were either stained with haematoxylin and eosin ...