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Citations for Leica :
501 - 550 of 830 citations for 3 Iodo 2 6 dimethyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of 3 µm thickness were cut at the level of the hippocampus using a microtome (Leica Microsystems, Wetzlar, Germany). For immunohistochemistry either paraffin sections that were washed with 0.5% sodium azide (2 × 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
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bioRxiv - Biophysics 2023Quote: ... and then glued onto Leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and sputter-coated with a 3 nm thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a Zeiss Auriga FEG-SEM (Zeiss ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 3 times with PBS and imaged using a Leica SP8 laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany) using a 63x water objective ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunohistochemical (IHC) staining for Estrogen receptor (ER) was conducted using the Leica Bond-3 automated stainer platform (Leica, Buffalo Grove, IL). Following antigen retrieval using a citrate-based pH 6 epitope retrieval solution ...
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bioRxiv - Cell Biology 2024Quote: ... was transferred to B-type planchettes (3 mm) and rapidly frozen with a Leica EM ICE high-pressure freezer (Leica Microsystems). The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4 ...
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bioRxiv - Microbiology 2024Quote: ... Cellular fluorescence signals in plant leaves agroinfiltrated with BiFC and other constructs expressing fluorescent proteins were observed at 3 dpi using a Leica SP8 laser-scanning confocal microscope (Leica, Germany) equipped with specific laser/filter combinations for YFP (excitation at 514 nm) ...
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bioRxiv - Neuroscience 2024Quote: ... the sucrose solution was removed and organoids were placed on OCT (at least 3 organoids on each OCT drop) and cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 20 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... brains were washed 3 times in PBS1X and sliced into coronal sections (50 µm) using a vibrating microtome (Leica Microsystems; VT1000S). Floating slices were maintained in PBS with azide at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... IHCs: 3 µm z-resolution) from the SGNs and IHCs were taken using a Leica SP8 confocal microscope (Leica microsystems, Germany) with a 40x objective ...
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bioRxiv - Microbiology 2024Quote: ... Images of single optical sections of about 0.5 μm thickness were captured with a confocal microscope and a 20x or 63x objective (Leica inverted 3).
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bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μm z-steps) were taken using a laser scanning confocal microscope (TCS SP8, Leica). Three sections of 20 μm depth each were averaged per animal for analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system (Leica).
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bioRxiv - Cell Biology 2020Quote: ... L4 animals were fixed by high-pressure freezing with the EMPACT-2 system (Leica Microsystems). Freeze-substitution (FS ...
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bioRxiv - Immunology 2021Quote: ... samples underwent heat induced antigen retrieval (HIER) in BOND Epitope Retrieval 2 (AR9640, Leica Biosystems) or BOND Epitope Retrieval 1 (AR9961 ...
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bioRxiv - Immunology 2024Quote: ... Heating mediated-epitope/antigen retrieval was performed with Bond TM Epitope Retrieval 2 (Leica Biosystems). Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST] ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
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bioRxiv - Bioengineering 2022Quote: ... and the appropriate visualization system was applied – DAKO DAB (Cat# K346811-2, Leica, Heidelberg, Germany) for all but CD206 which utilized AEC (Cat# K400511-2 ...
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bioRxiv - Cell Biology 2023Quote: Freeze substitution was performed in a Leica EM AFS-2 freeze substitution unit (Leica Microsystems) as described previously (Bragulat-Teixidor et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... the stained larvae were mounted in 2 % methylcellulose and photographed by a fluorescent stereomicroscope (Leica) or mounted in low-melting agarose gel and photographed by a confocal microscope (Olympus FV1000).
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bioRxiv - Cancer Biology 2024Quote: ... for transferrin receptor 1 (TfR1) and using Bond Epitope Retrieval Solution 2 (Leica Biosystems AR9640) for Ki67 and CD31 ...
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bioRxiv - Biochemistry 2024Quote: ... Grids were blotted for 2 s with an EM GP2 Plunge Freezing device (Leica Microsystems) held at 4 °C and 90% humidity before freezing in liquid ethane ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Biophysics 2024Quote: ... grids were applied with 3 μL 10 nm diameter BSA Gold Tracer (Aurion, The Netherlands) and single-side blotted using the EMGP plunger (Leica, Wezlar, Germany). Subsequent imaging of vitrified grids was performed on a Titan Krios microscope (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: All stained sections except for those from experiments involving Lepob mice (Fig. 3) were scanned at 20x magnification using a Leica Aperio AT2 slide scanner (Leica Microsystems, UK) prior to subsequent analysis of scanned slides as described below.
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bioRxiv - Neuroscience 2024Quote: ... Larvae subjected to TBI (as described above) at 3 dpf were anesthetized at 7 dpf with MS-222 and imaged under a fluorescent dissecting scope (Leica M165 FC). GFP+ puncta were counted from a lateral view (blinded).
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bioRxiv - Neuroscience 2024Quote: ... coli) and transferred onto 3% (w/v) agarose pads on a glass slide for confocal microscopy study (TCS SPE, Leica, Wetzlar, Germany). The excitation wavelength was set at 488 nm (Jex ∼488 nm) ...
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bioRxiv - Neuroscience 2024Quote: The paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 31 NC subjects) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany). Hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2024Quote: ... The immunostaining on each slice (3 sections per slice) was imaged using the Leica TCS SPE-II laser scanning confocal microscope (Leica, Wetzlar, Germany) and averaged per rat.