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Citations for Leica :
451 - 500 of 779 citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2019Quote: ... while the emitted fluorescence was recorded at 540/40 nm and 668/86 nm (Zeiss) or 520/23 nm and 700/90 nm (Leica) for GFP and mKATE ...
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bioRxiv - Neuroscience 2019Quote: ... Z stack images (0.685 µm thick optical sections, 20-23 sections) were obtained with a confocal microscope (Leica SP8, Leica-Microsystems) using a 20x objective (HC PL APO CS2 ...
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bioRxiv - Cell Biology 2021Quote: Tissues were embedded in OCT mounting media (Fisher HealthCare 23-730-571) and cut at 50 μm on a cryostat (Leica). Bones were fully decalcified in 14% EDTA ...
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bioRxiv - Immunology 2023Quote: ... as described previously.23 All heat induced epitope retrievals were performed with Bond™ Epitope Retrieval 2 (AR9640, Leica Biosystems) for 20 minutes at 95°C ...
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Cell Biology 2019Quote: ... submersed in the media chamber containing 4°C Phosphate buffered Saline and cut using a Leica VT1200S vibrating blade microtome (Leica Biosystems, UK) at a speed 0.3mm/sec ...
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bioRxiv - Cell Biology 2020Quote: Frozen 10-μm thick tissue cross-sections were cut from the mid-belly of the two lobes each sample at −20 °C with a cryostat (Leica, CM1950, Germany) and then stored at −80 °C for further staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Gelatine blocks were flash frozen at −80 °C in pre-cooled heptane and sectioned in 25 μm slices in a cryostat (Leica, CM3050 S). The slides dried overnight at room temperature and were mounted with mowiol and sealed with nail polish prior to confocal microscopy.
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bioRxiv - Biophysics 2020Quote: ... The blotted samples were immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunge freezer (Leica, EM GP). The vitrified specimens were then transferred into liquid nitrogen for storage ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Microbiology 2021Quote: ... pallidum strain Chicago C was thawed at room temperature and bacteria were enumerated on a Leica DM2500 darkfield microscope (Leica, Wetzlar, Germany) to inoculate 106 bacteria into each well of the 6-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... The slides were kept in a moist chamber (37°C) for subsequent incubation periods and further visualized with 63X oil immersion objective under a DMi8 microscope (Leica, Wetzlar, Germany) in the DIC mode.
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µm thick sections covering the injured area were produced using a cryostat maintained at -20°C (Leica CM 3050s, Techno-med). To assess the integrity of mitochondrial function the sections were sequentially stained for COX and SDH activities ...
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bioRxiv - Plant Biology 2020Quote: ... The number of protoplasts was counted with the C-Chip Neubauer improved hemocytometer (NanoEnTek, Seoul) under a light microscope (Leica VT1000, Wetzlar). The viability of protoplasts was determined using trypan blue solution (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... embedded in Tissue Tek® and after freezing at −80°C cut in 10 μm cross-sections using a Cryostat Microm HM560 (Leica Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... The frozen sample were transferred to cryovials in liquid nitrogen vapor and transferred to a pre-cooled (−90°C) freeze substitution unit (Leica EM AFS). Freeze substitution was performed using a mixture of 0.12% aqueous Uranyl acetate in anhydrous acetone using the following program ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S, Leica, Wetzlar, Germany) in 50 µm slices ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... frozen human brains were sliced at a temperature of −30 °C into 60 μm-deep coronal sections using a cryostat microtome (Leica Microsystems, Germany). Finally ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were postfixed in 4% paraformaldehyde overnight on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S), with sections collected into six tubes (containing cryoprotectant solution as described[32] ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2021Quote: ... the chambered slide was imaged using Leica SP5 inverted confocal microscope with environmental chamber to maintain the sample humidified at 37°C (Leica Microsystems, USA). Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... The grids were manually blotted using Whatman filter paper and plunge-frozen into liquid ethane at −174°C using a Leica EM-CPC equipment (Leica, Wetzlar, Germany). After freezing ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 at 10 °C and maintained at 90% humidity and then plunge frozen into liquid ethane (−180 °C) using a Leica GP plunger (Leica Microsystems, Austria).
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bioRxiv - Physiology 2022Quote: ... Samples were embedded in the Optimal Cutting Temperature compound and sectioned to 20–30 µm at 50 °C using a cryostat (Leica CM 3050S) and stained with haematoxylin & eosin using Sakuras Tissue-Tek DRS 2000 at the Department of Anatomy ...
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bioRxiv - Pathology 2022Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a SARS-CoV-2-infected hamster were used as positive assay controls.
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were frozen using dry ice to preserve at - 80°C and sectioned at a thickness of 40 μm using a cryostat (CM3050S, Leica Biosystems, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Microbiology 2022Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen to approximately -195 °C using an EM GP Plunge Freezer (Leica, Wetzlar, Germany).
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bioRxiv - Physiology 2023Quote: ... and imaged at 25°C in a glass-bottomed dish using an inverted confocal microscope (Leica SP8 DMi8, Leica Camera, Wetzlar, Germany) at 63x magnification (HC PL Apochromat C52 Oil objective ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Cell Biology 2023Quote: ... Seventy nm-thick frozen-hydrated sections were cut at −150°C in a Leica UC7/FC7 cryo-ultramicrotome (Leica Microsystems, Vienna, Austria). The EM grid was positioned underneath the ribbon using a Leica micromanipulator (Studer et al. ...