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Citations for Leica :
451 - 500 of 1510 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Images for Figure 4 were obtained using a THUNDER Imager (Leica Microsystems) maintained at 37°C and 5% CO2 equipped with a 63x/1.40-0.60 OIL Obj ...
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bioRxiv - Systems Biology 2023Quote: A 4-μm-thick tissue sections were cut using a microtome (Leica) and mounted onto the frame slides ...
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bioRxiv - Cancer Biology 2023Quote: ... Paraffin blocks were sliced at 4 µm thickness using LEICA RM2235 (Leica) and transferred to a specimen slide ...
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bioRxiv - Plant Biology 2024Quote: ... Representative images (Fig. 4) were obtained under 100 x objective magnification (Leica DM6 ...
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned at 4 μm thickness using a Leica microtome (Leica, Germany) (Kongthong et al. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Perfused brains (n = 20 / sex; 40 total) were sliced coronally into 6 series at 30 µm on a vibrating microtome (Leica VT 1000S). The samples were stored at -20 °C in cryoprotectant until immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 μm ...
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bioRxiv - Cell Biology 2021Quote: ... embedded in paraffin blocks and sectioned at 4 μm using a microtome (Leica). The sections were used for hematoxylin-eosin (H&E) ...
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bioRxiv - Immunology 2020Quote: ... 4 µm paraffin sections were subsequently cut in a microtome (Leica RM2235, Germany). Hematoxylin and Eosin staining was performed using standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... embedded in paraffin and sectioned (4 μm) at −20°C (RM2016, Leica, Germany).
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bioRxiv - Neuroscience 2024Quote: ... 4 μm sections produced with Leica RM2235 microtome (Leica Biosystems, Buffalo Grove, IL) were mounted on Superfrost Plus Microscope slides (#22-037-246 ...
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bioRxiv - Neuroscience 2022Quote: ... fixed in 4% paraformaldehyde solution and coronally sectioned with a vibratome (Leica VT1000). The slices were stained with Prussian blue and cresyl violet ...
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bioRxiv - Physiology 2023Quote: ... After fixation in 4% buffered formalin (Neutral Phosphate Buffered Formalin Fixatives, Leica, Germany), cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were sputter coated with 3-4 nm of platinum (Leica EM ACE600). Samples were imaged using a Helios Nanolab 660 DualBeam Microscope (FEI).
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bioRxiv - Neuroscience 2023Quote: ... then stained with 10uM 4-Di-2ASP and an epifluorescence microscope (Leica DMR) was used to visualize nerve terminals and muscle fibers ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 4% PFA and imaged by confocal microscopy (Leica SP8 equipped with a HC PL APO 63×/1.40 Oil CS2).
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Bioengineering 2023Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ ...
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bioRxiv - Bioengineering 2024Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ.
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...