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Citations for Leica :
451 - 500 of 538 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: 200 μm-thick hippocampal sections were isolated from 16 C57BL/6 mice of P22-28 using a vibratome (Leica, VT1200S), in the presence of ice-cold oxygenated a-CSF (125mM NaCl ...
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bioRxiv - Immunology 2022Quote: Tissue samples were fixed for a minimum of 7 days in 10% neutralized buffered formalin before being removed from BSL-4 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were fitted onto sample stubs using carbon adhesive tabs and sputter coated with 6 nm Iridium (Leica SCD500). Samples were imaged at 2 KV ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Microbiology 2021Quote: Tissue samples were fixed for a minimum of 24 h in 4% PFA before being removed from BSL-3 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Immunology 2020Quote: ... Paraffin blocks were sectioned from posterior to anterior at 6-μm thickness using a rotary microtome (Leica RM2255, Wetzlar, Germany). Sections were stained using Safranin-O/Fast Green at 90-μm intervals to assess cartilage morphology in the medial tibial plateau ...
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bioRxiv - Cell Biology 2023Quote: ... Transplanted embryos were mounted in 0.2% agarose in embryo medium and imaged between shield stage and 85% epiboly (6 - 9 hpf) under an inverted TCS SP8 confocal microscope (Leica) equipped with environmental chamber (Life Imaging Services ...
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Neuroscience 2024Quote: Coronal sections of the mouse brains were cut at a thickness of 6 μm via a microtome (Leica RM2245, Germany). Dewaxed and rehydrated hippocampal sections were blocked in TBST buffer (tris-buffered saline (TBS ...
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bioRxiv - Genetics 2024Quote: ... and processed into paraffin wax using a Tissue-Tek VIP 6 AI (Sakura) and finally embedded into paraffin wax blocks using a HistoCore Arcadia (Leica). The remaining hemisphere was further dissected into the hippocampal and cortex regions which were then placed into cryotubes and immediately snap frozen in liquid nitrogen.
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit, Leica Microsystems) for 60 min ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Neuroscience 2021Quote: ... 8–10 week old mice received a single impact onto the skull at bregma using an ImpactOne stereotaxic impactor (Leica Biosystems, Buffalo Grove, IL) with a 3-mm-diameter tip (velocity set at 4.0 m/s ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (8 μm) were cut on a cryostat HM 505E (Microm, Walldorf, Germany) equipped with a CryoJane Tape-Transfer system (Leica Inc., Buffalo Grove, IL). For labeling ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were sectioned into 6 µm thick cross sections with a Leica CM3050 S Research Cryostat (Leica Microsystems, Wetzlar, Germany), mounted on microscope slides and fixed with 1 % PFA in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... the scaffolds were embedded in paraffin wax and 6 µm thick slices were cut using a microtome (RM2125, Leica Microsystems, UK) and mounted on glass slides (Superfrost plus ...
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bioRxiv - Plant Biology 2021Quote: ... The root sections were mounted on slides in 50% glycerol. Wild type (cv. AMIGA) cluster root thin sections of 6 μm were produced using a microtome (RM2165, Leica Microsystems). They were counterstained for 5 min either with 0.05% toluidine blue or with 0.1% ruthenium red in 1X phosphate buffer saline (1X PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Neuroscience 2020Quote: ... transverse hippocampal slices (400 μm) were prepared from male C57BL/6 mice (10-12 weeks of age) using a vibratome (Leica, VT1200S). The CA3 region was cut ...
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bioRxiv - Bioengineering 2023Quote: ... The histological sections were taken at a thickness of 6 μm with a microtome (Leica RM2255 Microtome, Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... staining process involved serial repetitions of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems) or ER2 (Tris-EDTA buffer pH9 ...
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bioRxiv - Biophysics 2024Quote: Figure 6(a) depicts the schematic diagram of the proposed PCT that was built on a Leica microscope body (DMi8, Leica, Germany). 25 identical LEDs (470 ± 20 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Microphotographs (N>=6 fish per genotype) were acquired in a Leica DM4000 B microscope coupled to a Leica DFC425 C camera (Leica Microsystems).
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Immunology 2024Quote: ... tissues were subsequently embedded in OCT and stored at –80 °C before sectioning (6 μm) on a Leica CM1850 Cryostat (Leica Biosystems). Sections were incubated for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was imaged at 6 hours and 12 hours after seeding using a Leica DMI 4000 B Microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2024Quote: FFPE blocks of post-mortem human midbrains were sectioned at 6 µm thickness using a rotary microtome (HistoCore MULTICUT R Rotary, Leica Biosystems). The sections were mounted on Fisher Scientific slides (catalog #12-550-15 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... covered by 6 mm type B carrier (side flat) and frozen with a high-pressure freezing machine (EM ICE, Leica microsystems) as previously described112 ...
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bioRxiv - Neuroscience 2024Quote: ... were taken from 6 mice/group/sex using a DFC9000 GT VSC-12292 monochrome camera with Las X Navigator software (Leica Microsystems) at x20 magnification ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cancer Biology 2024Quote: ... Wound closure was measured at regular intervals (0-hour, 3-hour, 6-hour, 9-hour) using an inverted microscope (Leica DMi1). The wound closure distances at different time points were normalized to the distance at 0hour to calculate the migration rate.
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bioRxiv - Physiology 2020Quote: ... Sections of the arms and the central disk region (8 μm; transverse or horizontal) were cut using a microtome (RM 2145, Leica Microsystems [UK], Milton Keynes, UK) and mounted on chrome alum/gelatin coated microscope slides ...
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bioRxiv - Biophysics 2021Quote: ... Immersion Oil (Cargille laboratories, LDF, Code 387) was added onto the SU-8 layer before bringing the oil immersion objective (Leica, 63x, PL APO, 1.40 NA) manually in close proximity to the wafer surface ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were attached to SEM pin studs with conductive double-sided carbon tape and coated with 8 nm gold layers using a vacuum sputter coater (Leica EM ACE200 Low Vacuum Coater). The gold-coated samples were imaged using a Zeiss field emission scanning electron microscope (GeminiSEM 460 ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were imaged on a Leica Stellaris 8 microscope using a 10x/0.4 NA dry objective (Leica HC PL Apo CS2 10x/0.4 dry). A 460 nm laser line was used for excitation and a window of 469 nm - 600 nm was used for emission ...
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bioRxiv - Immunology 2024Quote: ... They were then sectioned at a thickness of 20 µm and imaged using the DAPI 10xPLFL-DRY channel to measure treatment fluorescence and the Spectrum Green 10xPLFL-DRY channel for background normalization on the Aperio Versa 8 (Leica Biosystems, South San Francisco, CA).
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bioRxiv - Bioengineering 2024Quote: ... The uniform tagging of 5-DTAF throughout the hydrogel was verified with multi-photon imaging from a point scanning confocal microscope (Leica Stellaris 8 DIVE, Leica; Germany).
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% low melting agarose and cut into slices of 100 μm with a vibratome Leica VT 1000S (Leica, Bensheim, Germany). The slices were washed twice in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The resin blocks were cut longitudinally into 6 μm slices of the femur distal metaphysis using a Leica SP1600 microtome (Leica Microsystems, Germany). Fluorescence signals of calcein and AR from the ROIs were captured using a fluorescence microscope (Q500MC ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...