Labshake search
Citations for Leica :
451 - 500 of 853 citations for 5 Nitrobenzothiazol 2 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS, Leica Biosystems Nussloch GmbH ...
-
bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS ...
-
bioRxiv - Cell Biology 2019Quote: ... Tissue was rinsed in MilliQ water for 5 minutes and then incubated in Hematoxylin 560 MX (Leica Biosystems, cat. 3801575) for 2 minutes and in running water for 1 minute ...
-
bioRxiv - Biochemistry 2019Quote: 5-micron FFPE sections were mounted onto MMI membrane slides (MMI, 50102) and stained using an automated stainer (Leica XL) at the Histology Core at University of Manchester ...
-
bioRxiv - Neuroscience 2019Quote: ... Rorb thickness was quantified by dividing the cortical plate into 5 equal bins and intensity in each bin was quantified using Aperio ImageScope (Leica).
-
bioRxiv - Plant Biology 2019Quote: ... cross sections 5 µm thick were obtained with an automatic advanced rotary microtome (model RM2155, Leica microsystems Inc., Deerfield, USA), were stained with toluidine blue then photographed using a digital camera (Zeiss AxioCam HRc ...
-
bioRxiv - Plant Biology 2019Quote: ... Semi-thin sections (5 μm thickness) for light microscopy were obtained with a microtome (HM 335E, Leica Microsystems, Heerbrugg, Switzerland) and stained with toluidine blue O.
-
bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
-
bioRxiv - Microbiology 2021Quote: ... imaging the colony and crystal formation at regular intervals at 5× magnification with a Leica MZ7.5 stereomicroscope (Leica, United Kingdom), using an Infinity 2 Lumenera microscope camera (Teledyne Technologies Inc. ...
-
bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
-
bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
-
bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Retinal cups were embedded in 5% agar moulds and immediately underwent sectioning on a fully automated vibratome (Leica VT1200 S). Sections were ‘Chased’ by incubation with 0.6 µM SNAP-Cell 647-SiR (New England Biolabs (S9102S ...
-
bioRxiv - Neuroscience 2022Quote: ... Horizontal slices containing the substantia nigra were cut at 222 μm in Krebs buffer bubbled with 95/5% O2/CO2 using a vibrating microtome (Leica). Slices were allowed to recover at 30°C in vials with 95/5% O2/CO2 Krebs with MK801 (10 μM ...
-
Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
-
bioRxiv - Bioengineering 2022Quote: PTFE surfaces were sputter-coated with platinum to develop a 5 nm thick conductive layer on the surface using an EM ACE600 sputter coater (Leica). Images were acquired using a Quanta 450 scanning electron microscope (FEI company).
-
bioRxiv - Biochemistry 2022Quote: ... serial 5 μm thick sections of adult scallop’s eye were obtained using a Leica CM1950 freezing microtome (Leica, Wetzlar, Germany) for Raman spectroscopy testing (alpha300R ...
-
bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
-
bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
-
bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
-
bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were imaged either with a Zeiss Observer A1 microscope and the AxioVision software 4.8 or with a STELLARIS 5 confocal microscope (Leica, Germany) at 40x (HC PL APO 40x/1.30 OIL CS2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 μm or 0.5 μm step sizes was performed on a Leica TCS sp8 Lightning Confocal/STED microscope coupled to LasX software (Leica) using a 63x (1.4 ...
-
bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 18.5 counting images were acquired using Leica Application Suite X (LAS X) on a Leica DMI8 fluorescent microscope from Leica Microscopy ...
-
bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
-
bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing OMM-Cepia3 and ER-mit RspA-splitFAST were mounted into an open-topped chamber in mKRB supplemented with TFCoral (5 µM) and imaged by a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
-
bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
-
bioRxiv - Plant Biology 2024Quote: ... The cells were then washed 3 times for 5 min with PBS and covered with PBS before imaging them with a TCS SP8 confocal laser-scanning microscope (Leica). Excitation/emission settings were 488 nm/498-524 nm HyD1 SMD hybrid detector for CAH4 ...
-
bioRxiv - Pathology 2023Quote: ... 5 μm thick sections were taken from the paraffin blocks using a rotary microtome (Leica RM2155, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin-eosin (HE) ...
-
bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
-
bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
-
bioRxiv - Molecular Biology 2024Quote: ... paraffin-embedded tissue was sectioned into approximate 0.5cm3 cubes and then cut into 5-μm slices by a LEICARM2235 Slice Machine (Leica, Germany). The samples were stained with haematoxylin/eosin (H/E ...
-
bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
-
bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
-
bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
-
bioRxiv - Neuroscience 2019Quote: Wide-field calcium imaging of Hydra was conducted at 2 Hz using a fluorescence dissecting microscope (Leica M165) equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC) ...
-
bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
-
bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
-
bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
-
bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.