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Citations for Leica :
451 - 500 of 1078 citations for 2 Chloro 4 3 fluoro 4 hydroxyphenyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Neuroscience 2024Quote: ... following imaging and either underwent cardiac perfusion with phosphate buffered saline and 4% paraformaldehyde or had their brains extracted and post-fixed in 4% paraformaldehyde before being cut on a vibratome in 40 µm sections (Leica VT1000S, Deer Park, IL). Sections through the olfactory bulb were mounted on slides and were coverslipped using Fluoromount-G containing DAPI (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Biochemistry 2024Quote: ... The excess liquid was blotted for 4 s prior to plunge-freezing into liquid ethane using a Leica EM GP2 plunger (Leica Microsystems; 95% humidity, 14 °C). Grids were stored in liquid nitrogen prior to data collection ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 spectral detectors (HyD-RLD, Leica) and a 37°C preheated 40X objective (NA 1.10 ...
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bioRxiv - Biochemistry 2022Quote: ... Pelco Easiglow) and vitrified (3 μL sample, 3 s blot time) using an automatic plunge freezer (Leica). EndoX-TSEN•CLP1 was frozen on R1.2/1.3 Cu 300 mesh grids (Quantifoil ...
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bioRxiv - Genomics 2024Quote: ... The nuclei were differentiated with mild acid treatment (Leica Biosystems 3803598), followed by “bluing” through exposure to a weakly alkaline solution (Leica Biosystems 3802918 ...
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bioRxiv - Cell Biology 2021Quote: ... Micro 3 - a DMi8 microscope (Leica Microsystems) with a high-contrast Plan Apochromat 63×/1.30 Glyc CORR CS objective (Leica Microsystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... GV-stage oocytes were injected with 10 pl of a solution containing 0.1 mM Emi2-3’UTR-MO or Emi2-3’UTR 5mm-MO using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium ...
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bioRxiv - Microbiology 2020Quote: ... coated with 3 nm gold/palladium (Leica CPD300) and examined with a JEOL JSM-7400F scanning electron microscope (3 kV-LEI detector).
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bioRxiv - Neuroscience 2023Quote: ... stained with 3% lead citrate (Leica UltroStain II) for 3 m ...
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bioRxiv - Immunology 2024Quote: ... the tip of the 3-mm impactor (Leica) piston was angled and kept perpendicular to the exposed cortical surface ...
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bioRxiv - Bioengineering 2024Quote: ... was added to the confocal dish with a 3 mm x 3 mm hole and observed under a confocal microscope (STEDYCON, Leica, Germany).
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bioRxiv - Plant Biology 2020Quote: ... Sections (3-4μm) cut by rotary microtome (Leica RM2265) were stained with 0.01 % aqueous toluidine blue ...
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Molecular Biology 2024Quote: ... 3 µm sections were cut on a microtome (Leica), rehydrated through an alcohol series according to standard laboratory procedures and then stained with a PAS staining kit (TCS Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µm sections were obtained with a microtome (Leica RM2255 Fully Automated Rotary Microtome) ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Microbiology 2024Quote: ... and IBA1 using the Bond- RX/3 autostainer (Leica) with the BPRD kit (DS9800 ...
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bioRxiv - Neuroscience 2023Quote: ... titrated to pH 7.4 with concentrated hydrochloric acid) using a VT1200s vibrating microtome (Leica). The transcardial perfusion procedure with 30 ml of chilled NMDG-HEPES aCSF was performed on P60 animals prior brain dissection and slicing ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Microbiology 2022Quote: ... Then tissues were sectioned to 3 μm by microtome (Leica). Next ...
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bioRxiv - Immunology 2021Quote: ... 3 x H2O) on a linear stainer (Leica Biosystems, ST4020). Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent ...
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bioRxiv - Biophysics 2022Quote: ... The 3D model in Figure 3 was analyzed by Leica LAS X ...
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bioRxiv - Neuroscience 2023Quote: ... Average power for excitation ranged from ∼35 mW to ∼80 mW measured at the front face of the objective (CA1 imaging: Nikon 16x, 3 mm WD, 0.8 NA; DG & CA3 imaging: Leica 25x, 3 mm WD, 1.0 NA). A 512 x 796 pixel field of view (FOV ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Developmental Biology 2020Quote: ... at least 3 transverse slices cut with a vibratome (VT1000S, Leica) at different anterior-posterior levels representing anterior ...
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bioRxiv - Neuroscience 2023Quote: ... and sections (3 µm) were cut using a microtome (LEICA RM2255). The sections were deparaffinized by bathing twice in xylene (for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were sectioned at 3 μm using a microtome (Leica, Germany) and the sections were stained with hematoxylin–eosin (HE ...
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bioRxiv - Systems Biology 2023Quote: ... we collected 3 10 µm sections using a Microtome (Leica Byostems). We then extracted RNA using Qiagen RNAse FFPE kit according to manufacturer instructions and evaluated the percentage of total RNA fragments longer than 200 nucleotides (DV200 ...
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Biochemistry 2020Quote: ... Confocal measurements were performed using a TCS SP8 STED 3× (Leica Microsystems) equipped with an HC PL APO CS2 100× objective (NA 1.4 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S ...
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bioRxiv - Cell Biology 2021Quote: ... Then 3-µm sections were made on a rotary microtome (RM2235, Leica). Slides were immediately stained for 1 minute in Mayer’s hematoxylin solution (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... sections cut to 3 µm thickness using a Leica microtome (Leica, Germany) and then stained using Quick Dip Stain Kit (Modified Giemsa Stain ...
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bioRxiv - Cell Biology 2021Quote: ... and infiltrated with wax through 3 Paraplast Plus paraffin baths (39602004; Leica). Samples were embedded in Paraplast Plus using paraffin-filled stainless steel base molds and a Thermo-Shandon Histocenter 2 Embedding Workstation (6400012D ...