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Citations for Leica :
451 - 500 of 1279 citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the samples were embedded in a mixture of OCT and water at a ratio of 1:1 and sectioned at 10 μm thickness with freezing microtome (Leica). The mRNA detection by in situ hybridization was performed as previously described (Lauter et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was trimmed to less than 1 × 1 mm and one side was exposed using an ultramicrotome (UC7, Leica), then turned downwards to be remounted to a metal pin with conductive silver epoxy (CircuitWorks ...
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The gonadal tissues for histology were dehydrated in a tissue processor (TP 1020-1-1, Leica Microsystems Nussloch GmbH, Germany) and thereafter embedded in paraffin ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Neuroscience 2022Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (25 μm-thick sections) using a Leica Cryostat (Leica, CM1860). For immunofluorescence staining ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted 1:100 in Antibody Diluent (Leica).
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bioRxiv - Immunology 2021Quote: ... anti-CD20 (clone L26, 1:200, Leica Biosystem), anti-CD31 (clone PECAM-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... CK14 (Novocastra/Leica; 1/50; NCL-L-LL02), CK8/18 (Novocastra/Leica ...
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bioRxiv - Developmental Biology 2020Quote: ... γ-sarcoglycane (1/150, G-SARC-CE, Leica biosystems), or with 4–15% Criterion™ TGX™ Precast Midi Protein Gel (5671084 ...
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bioRxiv - Immunology 2021Quote: ... or BOND Epitope Retrieval 1 (AR9961, Leica Biosystems) during 20 minutes for the adaptive and innate panel ...
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bioRxiv - Cell Biology 2021Quote: ... β-dystroglycan (1:50, LCL-b-DG, Leica Biosystem) and Ki67 (1:100 ...
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bioRxiv - Bioengineering 2023Quote: Primary Antibodies: anti-Dystrophin (Leica, NCLDYS1, 1:20), recombinant Anti-Lamin A + Lamin C antibody (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... GFAP (anti-GFAP (mouse, GA5, Leica, 1:200) and IBA1 (1:1000 Wako ...
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:200 in Antibody diluent (Leica). Brightfield images of 3-4 high-power field regions (40x ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:2500, Leica Biosystems, P53-PROTEIN-CM5), p21 (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:500, Leica Biosystems, NCL-p53-505), p19 (1:250 ...
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bioRxiv - Immunology 2022Quote: ... and CD163 (Leica Microsystems, 10D6, 1:20 dilution). Subsequent visualization was performed using Akoya Opal fluorophores (620 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Emerin (Leica NCL-Emerin; 1:1000). Secondary antibodies used ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-Vimentin (mouse, Leica, 1:100, cat#PA0640); anti-FAP (mouse ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit monoclonal ACE2 (1:400) and anti-αvβ6 integrin (1:3000) was diluted in Leica antibody diluent (RE AR9352, Leica, Biosystems, UK) and incubated with the sections overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 36 h post-transfection both the cell lines were trysinised and co-cultured in 1:1 ratio for 12 h followed by fixing permialization and subsequent imaging using fluorescence microscope (Leica Microsystems) and subsequent analysis by ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2021Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (18-20 μm-thick sections) using a Leica Cryostat (Leica, CM1850). Plated hSS cultures on glass coverslips were fixed in 4% PFA at room temperature for 10 minutes and washed with then rinsed twice for 5 minutes with PBS.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Bioengineering 2020Quote: ... 0.52 mM H2O2) (1:1 v/v) SHG images were acquired with a MaiTai multi-photon laser equipped confocal microscope (Leica SP8). SHG signal was collected with HyD detector between 437-453 nm (λex 880 nm ...
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bioRxiv - Physiology 2020Quote: ... after which they were incubated with antibodies against GPT (AP7468b, Abgent; 1:200) and GPT2 (PAD854Mu01, Cloud-Clone Inc.; 1:200) antigens in BONDTM primary antibody diluent (AR9352, Leica Biosystems). For skeletal muscle fiber size visualization ...
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bioRxiv - Genomics 2022Quote: ... cells were resuspended in 1× PBS and placed on an agarose pad (1% agarose in 1× PBS) and imaged with a laser scanning Leica TCS SP5 II confocal microscope (Leica Microsystems).
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bioRxiv - Plant Biology 2024Quote: ... The samples were transferred to a 1:1 (v/v) solution of 100% ethanol and solution A (Leica Historesin Embedding kit) and kept for 1h ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Cell Biology 2024Quote: ... Resin blocks were trimmed to approximately 2 mm2 and sectioned at 70 nm using a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc., Buffalo Grove, IL) and a Diatome diamond knife (Electron Microscopy Sciences ...
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bioRxiv - Biophysics 2024Quote: ... The grids with non-activated or IL33-activated (in total of 1 hr activation) human eosinophils were vitrified in liquid ethane using a Leica EM GP 1 (Leica Microsystems, Germany). The Leica EM GP 1 plunger was set to 37 ℃ ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Ki-67 (rabbit IgG, 1:2000, Leica Biosystems), anti-Nestin (rabbit IgG ...