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Citations for Origene Technologies :
1 - 50 of 77 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and cloned into pCMV-miR (Origene). To generate replication-deficient adenovirus ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA was obtained by RT-PCR and amplified through PCR using respective primers (OriGene Technologies) followed by agarose gel electrophoresis to assess the gene expression ...
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bioRxiv - Cell Biology 2021Quote: ... or a control (pCMV-MIR) purchased from OriGene. After 48 h or 72 h of culturing ...
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bioRxiv - Neuroscience 2020Quote: ... the cDNA containing its ORF was inserted into the pCMV-MIR vector (OriGene) to result in pCMV-Aβ175-cDNA ...
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bioRxiv - Cancer Biology 2022Quote: The plasmid DNA pCMV6-AC and the transfection control pCMV-MIR were purchased from OriGene (Maryland, EEUU). Caco-2 cells were plated (10·106 cells ...
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A mean-field approach for modeling the propagation of perturbations in biochemical reaction networksbioRxiv - Pharmacology and Toxicology 2021Quote: ... Chop mRNA levels in treated cells were measured using quantitative RT-PCR as previously described [28] using validated primers (OriGene, Cat # HP207450).
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bioRxiv - Genetics 2022Quote: ... DAB staining was performed using Polink-2 Plus HRP Polymer and AP Polymer detection for Rb antibody kit (D39-18, Origene) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... staining was performed using Polink-2 Plus HRP Polymer and AP Polymer detection for Rb antibody kit (D39-18, Origene, Washington, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Each well received 100 ng of the pMIR-REPORT™ Luciferase vector containing the 3’UTR-hTLR4 (without a negative control) in combination with 1µg pCMV-MIR or pCMV-pre-mir125b1 (OriGene Technologies, Rockville, Maryland, USA) or let7A2 generated in the laboratory and with 50 ng of pRL-TK Renilla luciferase plasmid (Promega). ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purified RT-DNA was prepared for sequencing by first treating with DBR1 (OriGene) to remove the branched RNA ...
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bioRxiv - Cancer Biology 2022Quote: Western blot antibodies for GOT2 detection were rabbit anti-human GOT-2 polyclonal antibody (Origene, catalog #TA325088) and HRP-conjugated AffiniPure Goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Gene-specific PCR primer pairs were obtained from OriGene Technologies (Rockville ...
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bioRxiv - Biophysics 2023Quote: ... flag-tagged mTHSD7A construct serving as the PCR template (Origene).
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bioRxiv - Immunology 2023Quote: Tissue paraffin sections were stained with H&E for histopathological evaluation or with biotinylated anti-mouse-IgG (Vector; BA-9200) for the detection of immune complex deposits and anti-human IL23A (OriGene; AM20386PU-N) for the detection of human IL23A protein in various tissues.
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bioRxiv - Biochemistry 2023Quote: ... PCR was performed using the human CYP4F2-myc-DDK (OriGene RC216427) plasmid (Forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... expression was carried out in healthy and cancer ovarian tissues by RT-qPCR using Tissuescan™ ovarian cancer cDNA arrays I-IV (Origene Technologies, USA) and EFA6R (NM_206909.3 ...
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bioRxiv - Immunology 2022Quote: ... The lentivirus containing media was harvested 72 h after transfection and concentrated 80 times using Lenti Concentrator (Origene). LV particles were then resuspended in RPMI 1640 media without serum and stored at −80°C before use ...
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bioRxiv - Molecular Biology 2020Quote: ... NSUN6 CDS was PCR amplified from the TrueORF pCMV-Entry vector (Origene) and cloned via Gibson assembly (NEB ...
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bioRxiv - Immunology 2019Quote: ... SCF and TPO were amplified by PCR from cDNA expression plasmids (Origene) and cloned into pMX retroviral vectors (vectors details in Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested PCR product was ligated into a pCMV6-Entry plasmid (Origene) with T4 DNA ligase ...
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bioRxiv - Microbiology 2020Quote: ... The murine Sel1L gene was PCR-amplified from pCMV6-Sel1L-Myc-DDK (OriGene) and subcloned in pcDNA3 ...
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bioRxiv - Neuroscience 2021Quote: ... Bcl6 (NM_009744) was cloned by PCR using a cDNA clone (Cat.No. MC203091, Origene) as template and inserted into CAG-CtlGFP to generate CAG-LSL-Bcl6GFP ...
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bioRxiv - Microbiology 2021Quote: ... the myc-DDK-tagged-RORC2 cDNA was PCR-amplified from plasmid RC212239 (Origene) and cloned into the MLV-based retroviral vector pMIG Blasti (gift of Jeremy Luban ...
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bioRxiv - Neuroscience 2019Quote: ... and human STAS (NM_015012) were generated by PCR using plasmid templates obtained from OriGene and cloned downstream of the CMV enhancer and chicken beta-actin (CB ...
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bioRxiv - Physiology 2021Quote: ... which was obtained by PCR from plasmid pCMV6-EIF2A-GFP (MG209105, OriGene, Rockville, MD) using forward primer 5’-ATTCGTCGACTGGATCCGGT-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were washed three times with PBS and incubated with secondary antibodies (hypersensitive enzyme-labeled goat anti-mouse/rabbit IgG polymer (OriGene, China) at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... full-length cDNA of TCF7L1 (NM_031283) was PCR-amplified from a commercial plasmid (Origene, SC126274) and an HA-tag was added by using AgeI-and NotI-restriction site containing primers (forward ...
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bioRxiv - Cancer Biology 2020Quote: Full-length BAP1 cDNA was amplified by PCR from pCMV6-AC BAP1 plasmid (Origene-SC117256) and cloned into the lentiviral plasmid pCCL-CMV-flT vector ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product and the plasmid pLenti-C-mGFP (# PS100071 OriGene Technologies, Rockville, MD, USA) were digested with Asc1 (#R0558L Bioconcept ...
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bioRxiv - Immunology 2020Quote: ... washing with PBST three times, mouse anti-HA-tag lgG2a mAb (4A.Biotech, 4ab000002, Beijing, China 1:1000) or lgG1 mAb (Origene, TA180128, USA, 1:2000) were added into each well (40 μl/well) ...
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bioRxiv - Biophysics 2021Quote: ... DNA encoding ctSTIM1 (aa 233-685) was amplified by PCR from full-length human STIM1 (Origene), appending an N-terminal NcoI cleavage site ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA templates were made by PCR amplification from a plasmid pCMV6-mNlgn2(A+)-mycDDK (Origene, catalog #MR222168 ...
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bioRxiv - Cell Biology 2023Quote: ... the KPNB1 coding sequence was PCR amplified from the KPNB1 ORF clone plasmid (Origene, cat# RC200659) and XbaI and BamHI restriction sites were added to the ends of the fragment ...
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bioRxiv - Cell Biology 2019Quote: ... BMP2K (1-560) and BMP2K (561-1161) were PCR amplified from the template ORF clone # RC215795 (Origene) and inserted into HindIII restriction site of pEGFP-N1 vector using In-Fusion cloning technology (Clontech ...
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bioRxiv - Immunology 2024Quote: Quantitative PCR (qPCR) was performed on cDNA panels of 48 healthy human tissues (OriGene Technologies, Rockville, MD) and TNBC cell lines using MX3000 (Taqman probes ROPN1 ...
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bioRxiv - Cell Biology 2024Quote: ... A commercial Cotinine ELISA kit (Origene) suitable for mice was used to quantify blood levels ...
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bioRxiv - Cancer Biology 2020Quote: ... The canonical RIPK2 coding sequence (NM_003821) and MKK7 (i.e., MAP2K7) coding sequence (NM_145185.4) were amplified by PCR from a RIPK2 vector (Origene, #RG202530) and a MKK7 vector (GenScript ...
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bioRxiv - Biochemistry 2019Quote: ... the coding region for full-length human GCP2 (amino acids 1-902) was amplified by PCR using its cDNA as template (NM_001256617.1, Origene). The mTagBFP (blue fluorescent protein ...
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bioRxiv - Microbiology 2021Quote: The open reading frame of human MIF was amplified by PCR from the vector pCMV6-entry MIF (Origene) and cloned into the pET11a vector (Novagen ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... the coding sequence without stop codon was amplified by PCR from the Myc-DDK-tagged CDK5RAP3 (tv3) plasmid (Origene Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The different fragments were PCR amplified using custom designed primers representing the 5’ and 3’ sequence respectively with Tau cDNA (RC213312, Origene) as template ...
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bioRxiv - Microbiology 2020Quote: ... hnRNPUL1 (NM_007040) and PEG10 (NM_015068.3) were PCR amplified from ORF clones (hnRNPL, GenScript #OHu14072; hnRNPUL1, Origene #RC200576; PEG10, GenScript #OHu101111) and the products were cloned into the mammalian expression plasmid pCAGGS ...
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bioRxiv - Developmental Biology 2021Quote: A synthetic DNA construct encoding mouse ACVR1 with the R206H mutation (G to A at nucleotide position 617) was assembled from synthetic oligonucleotides and PCR products and cloned into the pCMV6 entry mammalian expression vector (Origene), which also encodes a neomycin resistance marker ...
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bioRxiv - Genetics 2019Quote: A CrispR-Cas9 knockout kit (Origene, Rockville, MD) for mouse Kif26b (SKU KN308785) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid pCMV6-AC-GFP-rtTA-APE1 (for WT tGFP-APE1 protein) was prepared by PCR full-length APE1 from pET28HIS-hAPE1 and subcloned into pCMV6-AC-GFP-rtTA vector (Origene #PS100125) at AscI and RsrII sites ...
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bioRxiv - Cell Biology 2022Quote: ... using Lenti-vpak Lentiviral Packaging Kit (Origene Technologies, Inc.). The HEK 293T cells were expanded in DMEM (high glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... C-kit Variant 2 human ORF cDNA clone (Origene, Rockville ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse Irx3 (NM_008393) was amplified by PCR from the donor vector pCMV6-entry-Irx3-myc-DDK (cat. no MR208149, Origene, Rockville, MD, USA) with the following forward 5’-CGATCTAAGTAAGCTTCACCATGTCCTTCCCCCAGCTCG-3’ and reverse 5’-GATCTTGGCAAAGCTTAGACGAGGAGAGAGCTGATAAGACC-3’ primers ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR/Cas9 knockout (KO) kit was purchased from Origene (KN206895) and cell lines were generated following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The lentivirus concentration was quantified by Lenti ELISA Kit (Origene). After 16h ...