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Citations for Sarstedt :
1 - 50 of 106 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... LipA was aliquoted in reaction tubes (Low Protein Binding, Sarstedt), flash-frozen and stored at −80°C.
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bioRxiv - Bioengineering 2022Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany). The 1 mg/mL stock solutions were further diluted to working solutions consisting of 10 ng/μL human or bovine insulin ...
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bioRxiv - Biochemistry 2023Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany).
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bioRxiv - Microbiology 2020Quote: ... Buffer was pre-mixed with proteins in low-binding microtubes (Sarstedt) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The urea-denatured LipA-FM was diluted with the TGCG buffer from 150 μM stock solution to 1.6 μM and kept on ice protein in reaction tubes (Low Protein Binding, Sarstedt). The chaperones were transferred to 1.5 mL polypropylene reaction tubes containing 20 mM Tris-HCl pH 8.0 and varying NaCl concentrations ...
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bioRxiv - Biochemistry 2023Quote: ... Binding assays were performed in Low binding micro tubes (Sarstedt). Supernatant and pellet fractions were mixed with protein sample buffer and analyzed by Coomassie staining after SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of 50 nM LipA were mixed with Skp ranging from to 2.3 nM to 18.5 μM in a 0.5 mL reaction tube (Low Protein Binding, Sarstedt). LipA:Skp samples were incubated for 15 min at 22 °C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... proteins of interest at a concentration of 3µM were pre-mixed in a low-binding microtube (Sarstedt) and incubated for 10min at 25°C before application onto the EM grid ...
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bioRxiv - Cell Biology 2023Quote: ... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells grown in 96-well plates (Sarstedt) were preloaded with 1 µM Fura-2-AM/ 0.02% Pluronic® F127 (P-3000MP ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed together at concentration 1 μM in TGCG buffer to form lipase:foldase complexes in reaction tubes (Low Protein Binding, Sarstedt). The complex formation was set for 15 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The total volume was set to 65 μL and the reaction was carried out at room temperature in reaction tubes (Low Protein Binding, Sarstedt). For the control experiments ...
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bioRxiv - Microbiology 2023Quote: ... a white high-binding polystyrene plate (Sarstedt) was used ...
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bioRxiv - Immunology 2023Quote: 96-well high-binding ELISA plates (Sarstedt) were coated overnight at 4°C with either goat anti-mouse IgA ...
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bioRxiv - Biochemistry 2023Quote: ... and manufacturer C (SafeSeal reaction tube no. 72.706 and SafeSeal reaction tube, Low protein-binding no. 72.706.600, Sarstedt AG & Co. KG, Nümbrecht, Germany). Further ...
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bioRxiv - Microbiology 2022Quote: ... Low retention pipette tips and low binding Eppendorf tubes (Sarstedt) were used throughout.
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bioRxiv - Microbiology 2023Quote: ... Low retention pipette tips and low binding Eppendorf tubes (Sarstedt) were used throughout ...
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bioRxiv - Molecular Biology 2023Quote: Cell lines (HEK293, HeLa and HepG2) were cultured as adherent monolayers in filter-capped plastic flasks (Corning or Sarstedt) in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2024Quote: ... and loaded onto a 96-well high binding ELISA plate (Sarstedt Inc. Montreal, QC) covered with plastic wrap ...
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bioRxiv - Immunology 2023Quote: ... Resulting PBMCs were cultured at 1×106 cells/mL in 96-well high binding plates (Sarstedt, USA) pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL ...
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bioRxiv - Microbiology 2023Quote: L929 fibroblasts were rapidly thawed in a 37°C water bath before being washed once in DMEM and 10% HI-FBS and transferred to a T-25 tissue culture treated flask (Sarstedt, Nümbrecht, Germany) in 10 mL DMEM and 10% HI-FBS with 100 U/mL penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated sample was centrifuged (15000 x g, 30 min, 4 °C) and the supernatants were transferred into fresh DNA-low binding tubes (Sarstedt, Cat.-No. 72.706.700). The protein concentration was measured using Bradford assay (Roti®-Quant ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocytes were seeded into 6-well plates (Sarstedt, 83.3920) at 100,000 cells/well and either live-imaged or serially imaged as indicated ...
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bioRxiv - Microbiology 2024Quote: ... Samples of 2 ml were collected in 2 ml microtubes (Sarstedt, ref 72.690.001) 180 min after the addition of the compounds and centrifuged at 13000 x g for 2 min ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred into 2 ml reaction tubes (Sarstedt), and cells were isolated by centrifugation at 5000×g for 8 minutes in a cooled table-top centrifuge ...
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bioRxiv - Molecular Biology 2021Quote: ... at a final concentration of 2×108 cells/ml in 2 ml screw cap microtubes (Sarstedt, Cat. No. 72.693). To assess the translation activity of lysates from frozen cells ...
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bioRxiv - Cell Biology 2022Quote: ... the pTr1 cells (2×105 cells/2 ml/well) were seeded in 6-well plates (83.3920; Sarstedt, Nümbrecht, Germany) in CM until the monolayer reached 80-90% confluence ...
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bioRxiv - Immunology 2022Quote: ... human blood was collected in tubes containing anticoagulant citrate (S-monovette #04.1902, Sarstedt, Nümbrecht, Germany). Neutrophils were isolated using a Percoll-based protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... added to 2 ml reaction tubes (Sarstedt, Germany) and the particulate matter immediately pelleted by centrifugation at 14 000 x g (Hermle Z 233 M-2 ...
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bioRxiv - Plant Biology 2019Quote: ... the supernatant was transferred to 2 ml tubes (Sarstedt) containing 1.7 ml milliQ H2O to polarize the solution ...
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bioRxiv - Microbiology 2020Quote: ... transferred in 2 ml eppendorfs (SARSTEDT AG & Co. KG), and centrifuged at 17000 x g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and transferred to 2-mL screw tubes (SARSTEDT, 72.693). The flies were homogenised with Precellys 24 (Bertin Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... placed in 2 ml polypropylene vials (Sarstedt AG and Co.) and stored at -80°C until processing.
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bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT) inside a 1 ml Polystyrene Cuvette (Sarstedt). MDH activity was quantified by measuring absorption at 340 nm every 10 s for 30 s on a Biochrom Novaspec Plus photometer ...
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bioRxiv - Molecular Biology 2020Quote: ... The worms were transferred to 2 ml tubes (Sarstedt, Ref: 72.693.005) with Zirconia/Silica beads (Biospec ...
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bioRxiv - Evolutionary Biology 2020Quote: ... supernatants were transferred to fresh 2 ml plastic vials (Sarstedt, Germany). Extractions were repeated once more with pure methanol ...
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bioRxiv - Microbiology 2021Quote: ... were added to 2 mL screw cap micro tubes (Sarstedt Inc). 60 μL of resuspended cells were added to the tubes and bead beating was performed at max speed for 20 seconds in FastPrep FP120 cell disruptor ...
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bioRxiv - Plant Biology 2021Quote: ... 1.5 mL were transferred to a 2 mL reaction vessel (Sarstedt). The bacteria were spun down at 3930 x g (Rotanta 96R ...
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bioRxiv - Zoology 2022Quote: ... stored in 2 mL screw cap microtubes (Sarstedt Inc., Nümbrecht, Germany) and frozen for transportation ...
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bioRxiv - Microbiology 2021Quote: ... CD14+ cells are purified by magnetic bead separation of PBMCs using CD14+ human positive selection kit (StemCell) and plated 1M cells per ml on teflon plates (Sarstedt) with 7 ml per plate in the following medium ...
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bioRxiv - Bioengineering 2023Quote: Human hepatic cells from hepatocellular carcinoma (HepG2 cells, ATCC, Manassas, Virginia, USA) were maintained in T25 flasks (Sarstedt, Numbrecht, Germany) under standard conditions (37 °C ...
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bioRxiv - Microbiology 2024Quote: ... All stool and tissues were harvested into 2-mL tubes (Sarstedt, Germany) with 1-mm-diameter zirconia/silica beads (Biospec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Approach 1 (human serum): Whole Blood was obtained from healthy adult volunteers and collected into Monovettes (7.5 ml, K3 EDTA, Sarstedt, Nümbrecht, Germany). Procedures were approved by the institutional review board (IRB ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5% human platelet lysate, and 1 IU/ml heparin (both PL BioScience, Aachen, Germany) on standard T-flasks (Sarstedt, Nümbrecht, Germany), in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: 2×106 bone marrow cells were cultured in non-treated 10cm plates (Sarstedt) in 10 ml VLE DMEM (Biochrom ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 g stool were collected into faeces collection tubes (Sarstedt, Nümbrecht, Germany), put at 4 °C and diluted fivefold in 1 x PBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The cell pellets were transferred to a sterile 2 ml tube (Sarstedt, Germany) containing 100 mg RNAse-free 0.1 mm silica beads (Biospec ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were placed in 2-mL polypropylene vials (Sarstedt AG & Co., Nümbrecht,Germany) containing five zirconium ceramic oxide beads (1.4-mm diameter ...
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bioRxiv - Systems Biology 2023Quote: ... For each passage 1-2×106 cells were seeded in T25 flasks (Sarstedt). Then ...
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bioRxiv - Microbiology 2023Quote: ... Suspended samples were finally transferred to 2 ml DNA LowBind micro tubes (Sarstedt) for following treatments using cut filter tips to reduce possible shearing ...