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Citations for Sarstedt :
1 - 50 of 113 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
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bioRxiv - Microbiology 2021Quote: ... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
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bioRxiv - Biochemistry 2023Quote: ... and manufacturer C (SafeSeal reaction tube no. 72.706 and SafeSeal reaction tube, Low protein-binding no. 72.706.600, Sarstedt AG & Co. KG, Nümbrecht, Germany). Further ...
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bioRxiv - Microbiology 2023Quote: ... Purified virus stock in PBS was spiked in 100 μl of the matrix in a 1.5-ml plastic tube (Sarstedt, 3080521), to achieve a starting concentration in the experimental solution of either 107 PFU/ml (“normal titer ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5% human platelet lysate, and 1 IU/ml heparin (both PL BioScience, Aachen, Germany) on standard T-flasks (Sarstedt, Nümbrecht, Germany), in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Physiology 2021Quote: Adult flies of the desired genotype were collected and aged for 6 days at 25°C and transferred into 1% agar (in dH2O) contained in 12 mL vials (SARSTEDT, 58.487). Dead animals were counted in 15 ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed together at concentration 1 μM in TGCG buffer to form lipase:foldase complexes in reaction tubes (Low Protein Binding, Sarstedt). The complex formation was set for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocytes were seeded into 6-well plates (Sarstedt, 83.3920) at 100,000 cells/well and either live-imaged or serially imaged as indicated ...
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bioRxiv - Bioengineering 2022Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany). The 1 mg/mL stock solutions were further diluted to working solutions consisting of 10 ng/μL human or bovine insulin ...
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bioRxiv - Biochemistry 2023Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany).
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bioRxiv - Biochemistry 2022Quote: ... The urea-denatured LipA-FM was diluted with the TGCG buffer from 150 μM stock solution to 1.6 μM and kept on ice protein in reaction tubes (Low Protein Binding, Sarstedt). The chaperones were transferred to 1.5 mL polypropylene reaction tubes containing 20 mM Tris-HCl pH 8.0 and varying NaCl concentrations ...
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bioRxiv - Immunology 2022Quote: ... human blood was collected in tubes containing anticoagulant citrate (S-monovette #04.1902, Sarstedt, Nümbrecht, Germany). Neutrophils were isolated using a Percoll-based protocol ...
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bioRxiv - Biochemistry 2022Quote: ... LipA was aliquoted in reaction tubes (Low Protein Binding, Sarstedt), flash-frozen and stored at −80°C.
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bioRxiv - Neuroscience 2021Quote: ... for 5 min at 4°C in Micro tube 1.1ml Z-Gel (Sarstedt, Germany). Samples were kept on ice at all times ...
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bioRxiv - Microbiology 2022Quote: ... 22 or 25 °C was split into two new 50 mL flasks (Sarstedt Germany) with one used as a control and the other flask supplemented with the ribosome blocker lincomycin (0.5 mg mL−1 final concentration ...
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bioRxiv - Molecular Biology 2023Quote: Atu strains were precultured overnight at 28°C in 14 ml polypropylene tubes (Sarstedt) in a rotary shaker ...
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bioRxiv - Microbiology 2020Quote: ... Buffer was pre-mixed with proteins in low-binding microtubes (Sarstedt) on ice ...
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bioRxiv - Cancer Biology 2023Quote: Biopsies were removed from −80°C and placed immediately into 2 mL microtubes (Sarstedt, Nuembrecht, Germany) filled to ∼1/3 volume with 2 and 0.1 mm diameter zirconia beads (Biospec ...
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bioRxiv - Microbiology 2020Quote: ... The strains were incubated at 25 °C for 24 hours in 25 cm2 cell culture flasks (Sarstedt). The strains were then diluted 1:10 in Fraquil and 1 ml were distributed in 13-ml tubes (Sarstedt ...
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bioRxiv - Microbiology 2021Quote: ... CD14+ cells are purified by magnetic bead separation of PBMCs using CD14+ human positive selection kit (StemCell) and plated 1M cells per ml on teflon plates (Sarstedt) with 7 ml per plate in the following medium ...
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bioRxiv - Bioengineering 2023Quote: Human hepatic cells from hepatocellular carcinoma (HepG2 cells, ATCC, Manassas, Virginia, USA) were maintained in T25 flasks (Sarstedt, Numbrecht, Germany) under standard conditions (37 °C ...
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bioRxiv - Microbiology 2020Quote: ... and inoculated in 500 µL MSgg medium inside an 8-well tissue culture chamber at 30°C (Sarstedt; width ...
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bioRxiv - Immunology 2022Quote: ... Samples were stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany), but were equilibrated to room temperature before injections ...
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bioRxiv - Neuroscience 2023Quote: ... the organoid pieces were digested in 2 ml of CellLive™ Tissue Dissociation Solution (#1190062, Singleron Biotechnologies,) at 37°C for 15 minutes in a 15-ml centrifuge tube (#62.5544.003, Sarstedt,), with continuous agitation on a thermal shaker ...
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bioRxiv - Plant Biology 2024Quote: ... 4°C) and the supernatant was filtrated through a 0.2 µm syringe filter (cat-no. 83.1826.001; Sarstedt, www.sarstedt.com). The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Approach 1 (human serum): Whole Blood was obtained from healthy adult volunteers and collected into Monovettes (7.5 ml, K3 EDTA, Sarstedt, Nümbrecht, Germany). Procedures were approved by the institutional review board (IRB ...
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bioRxiv - Biochemistry 2023Quote: ... Thermo Fisher Scientific).100 µg of protein was aliquoted to a 1.5 mL screw top tube (72.703.600, Sarstedt) and digested using the EasyPep Mini MS Sample Prep Kit (A40006 ...
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bioRxiv - Systems Biology 2020Quote: ... The plates were incubated for 16 h at 37 °C (horizontally shaking at 180 rpm) and rearranged into one 96-well plate (MegaBlock 96 Well, 2.2 ml, Sarstedt). 110 Ml/well of bacteria was used to create glycerol stock plate (Round 96 Well Storage Plates ...
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bioRxiv - Microbiology 2022Quote: ... Viral culture supernatant was harvested 96 hrs post-infection and clarified then stored at −80 °C in 500 μL aliquots in 2mL cryovials (72.694.406, Sarstedt, Inc.) until required ...
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bioRxiv - Microbiology 2023Quote: ... The cell debris was removed by centrifugation at 12,000 × g for 15 min at 4°C and the supernatant filtered through a 0.45 μm pore size Filtropur S membrane (Sarstedt). The cleared extract was directly loaded onto a HisTrap FF crude column (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: Barcoded Asaia bacteria were grown overnight at 30°C to early log phase (OD600 0.5-0.8) in a deep-well plate (Sarstedt, Nümbrecht, Germany) in 300 μl GLY medium supplemented with 100 μg/ml kanamycin per well ...
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bioRxiv - Microbiology 2022Quote: ... GG770 and LL1021 cells were grown aerobically at 37 °C under agitation in 5 ml of M9 minimal medium in 50 ml conical polypropylene tubes (Sarstedt) with an inclination of 90° ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were recovered for 5 hours at 30°C in YP medium with 2% maltose and then spread onto 50 petri dishes (150×20 mm, Sarstedt) with YNB + CSM Ura− with 2% maltose supplemented with G418 at 0.2 mg mL−1 ...
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bioRxiv - Microbiology 2019Quote: ... Cultures were inoculated to an initial OD600 of 0.05 into fresh medium from overnight liquid cultures grown in LB in 18mm borosilicate culture tubes at 37°C on a roller drum in 15 mL conical screw-cap tubes (Sarstedt). Tubes were placed in 2.5 liter AnaeroPack System rectangular jars (Mitsubishi Gas Chemical Co. ...
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bioRxiv - Neuroscience 2023Quote: ... Samples of each peptide and the peptide mix were incubated at 37 °C in sealed 2 ml non-treated plastic cryotubes (Sarstedt) for 24 hours and amyloid content was determined by ThT fluorescence and TEM (data not shown).
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bioRxiv - Microbiology 2019Quote: The level of pyoverdine and pyocyanine production was analyzed on a 72h-biofilm formed on Nephrophane or selected PAO1 mutants cultured (48 h, 37°C) in TSB medium (bioMerieux, France) in a 24-well polystyrene plate (Sarstedt, Germany) (Danis-Wlodarczyk et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... D and E) or as catheter urine from a fresh collection bag (timepoint B and C) into standard urine sample cups (Sarstedt, Germany). Preservatives and protease inhibitors were added to the final concentrations 6,6mM NaN3 ...
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bioRxiv - Immunology 2022Quote: ... the unstressed sample was prepared once weekly and stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany). The sample was used for two rounds of injections and hereafter discarded.
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bioRxiv - Microbiology 2023Quote: ... where a large volume of passage one stock was made and stored at -80°C in 500 μL aliquots in 2 ml cryovials (72.694.406, Sarstedt Inc. (Nümbrecht, Germany)) until required ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial growth was determined after 24 h of incubation (37°C, 200 rpm) by measuring at OD600 using standard photometer cuvettes (Sarstedt, Germany). An identical protocol was applied for the 20 clinical NDM-producing isolates with the only modification of using a fixed concentration of 4 µg/ml of aztreonam.
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of 50 nM LipA were mixed with Skp ranging from to 2.3 nM to 18.5 μM in a 0.5 mL reaction tube (Low Protein Binding, Sarstedt). LipA:Skp samples were incubated for 15 min at 22 °C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... proteins of interest at a concentration of 3µM were pre-mixed in a low-binding microtube (Sarstedt) and incubated for 10min at 25°C before application onto the EM grid ...
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bioRxiv - Microbiology 2023Quote: ... Parents were instructed to collect infant faeces from diapers and freeze them immediately at –20◦C in their home freezer inside a cool transport container (Sarstedt, Hilden, Germany). Samples were transported to the laboratory ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: ... the organoid pieces were digested in 2 ml of CellLive™ Tissue Dissociation Solution (Singleron Biotechnologies, #1190062) at 37°C for 15 minutes in a 15-ml centrifuge tube (Sarstedt, #62.5544.003), with continuous agitation on a thermal shaker ...
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bioRxiv - Neuroscience 2023Quote: We collected blood from ad-libitum fed 9 months-old mice through cardiac puncture and centrifugation at 2000 g for 5 minutes at 4 C° in heparinised tubes (Microvette CB 300; Sarstedt, Leicester, UK) for plasma collection ...
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bioRxiv - Microbiology 2019Quote: ... and used to inoculate 200 µL of M9 medium supplemented with the desired C-source in a 96-well microtiter plate (Falcon, product no. 353047 or Sarstedt, product no. 83.3924) and incubated at 30°C and 250 rpm in a microplate reader (Xenius ...
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bioRxiv - Genetics 2022Quote: ... Blood was collected by venous puncture or cardiac puncture at the time of sacrifice and centrifuged at 2000 g for 15 minutes at 4 °C in heparin-coated tubes (Sarstedt AG, Nürnbrecht, Germany) to separate plasma from cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated sample was centrifuged (15000 x g, 30 min, 4 °C) and the supernatants were transferred into fresh DNA-low binding tubes (Sarstedt, Cat.-No. 72.706.700). The protein concentration was measured using Bradford assay (Roti®-Quant ...