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Citations for Sarstedt :
1 - 50 of 86 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... HLA peptides were eluted into 1.5mL micro tubes (Sarstedt, Nümbrecht, Germany), frozen ...
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bioRxiv - Microbiology 2023Quote: ... HLA peptides were eluted into 1.5 mL micro tubes (Sarstedt, Nümbrecht, Germany), frozen ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells grown in 96-well plates (Sarstedt) were preloaded with 1 µM Fura-2-AM/ 0.02% Pluronic® F127 (P-3000MP ...
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bioRxiv - Molecular Biology 2023Quote: Cell lines (HEK293, HeLa and HepG2) were cultured as adherent monolayers in filter-capped plastic flasks (Corning or Sarstedt) in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5% human platelet lysate, and 1 IU/ml heparin (both PL BioScience, Aachen, Germany) on standard T-flasks (Sarstedt, Nümbrecht, Germany), in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: L929 fibroblasts were rapidly thawed in a 37°C water bath before being washed once in DMEM and 10% HI-FBS and transferred to a T-25 tissue culture treated flask (Sarstedt, Nümbrecht, Germany) in 10 mL DMEM and 10% HI-FBS with 100 U/mL penicillin/streptomycin ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed together at concentration 1 μM in TGCG buffer to form lipase:foldase complexes in reaction tubes (Low Protein Binding, Sarstedt). The complex formation was set for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocytes were seeded into 6-well plates (Sarstedt, 83.3920) at 100,000 cells/well and either live-imaged or serially imaged as indicated ...
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bioRxiv - Bioengineering 2022Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany). The 1 mg/mL stock solutions were further diluted to working solutions consisting of 10 ng/μL human or bovine insulin ...
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bioRxiv - Biochemistry 2023Quote: ... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany).
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bioRxiv - Biochemistry 2022Quote: ... The urea-denatured LipA-FM was diluted with the TGCG buffer from 150 μM stock solution to 1.6 μM and kept on ice protein in reaction tubes (Low Protein Binding, Sarstedt). The chaperones were transferred to 1.5 mL polypropylene reaction tubes containing 20 mM Tris-HCl pH 8.0 and varying NaCl concentrations ...
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bioRxiv - Immunology 2022Quote: ... human blood was collected in tubes containing anticoagulant citrate (S-monovette #04.1902, Sarstedt, Nümbrecht, Germany). Neutrophils were isolated using a Percoll-based protocol ...
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bioRxiv - Biochemistry 2022Quote: ... LipA was aliquoted in reaction tubes (Low Protein Binding, Sarstedt), flash-frozen and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... Buffer was pre-mixed with proteins in low-binding microtubes (Sarstedt) on ice ...
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bioRxiv - Microbiology 2021Quote: ... CD14+ cells are purified by magnetic bead separation of PBMCs using CD14+ human positive selection kit (StemCell) and plated 1M cells per ml on teflon plates (Sarstedt) with 7 ml per plate in the following medium ...
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bioRxiv - Bioengineering 2023Quote: Human hepatic cells from hepatocellular carcinoma (HepG2 cells, ATCC, Manassas, Virginia, USA) were maintained in T25 flasks (Sarstedt, Numbrecht, Germany) under standard conditions (37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Approach 1 (human serum): Whole Blood was obtained from healthy adult volunteers and collected into Monovettes (7.5 ml, K3 EDTA, Sarstedt, Nümbrecht, Germany). Procedures were approved by the institutional review board (IRB ...
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bioRxiv - Biochemistry 2023Quote: ... Thermo Fisher Scientific).100 µg of protein was aliquoted to a 1.5 mL screw top tube (72.703.600, Sarstedt) and digested using the EasyPep Mini MS Sample Prep Kit (A40006 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of 50 nM LipA were mixed with Skp ranging from to 2.3 nM to 18.5 μM in a 0.5 mL reaction tube (Low Protein Binding, Sarstedt). LipA:Skp samples were incubated for 15 min at 22 °C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... proteins of interest at a concentration of 3µM were pre-mixed in a low-binding microtube (Sarstedt) and incubated for 10min at 25°C before application onto the EM grid ...
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bioRxiv - Cell Biology 2023Quote: ... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
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bioRxiv - Bioengineering 2023Quote: ... BSA standard and purified protein (25 μL) were each added in triplicate to a microtiter plate (Sarstedt, Nümbrecht, Germany) and 200 μL of a 50:1 mixture of BCA and copper(II ...
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bioRxiv - Developmental Biology 2022Quote: Cytoplasmic protein level: All were measured in live germline cells performed by mounting the testes on 50 mm lummox dish (Sarstedt) in a drop of S2 media ...
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bioRxiv - Biochemistry 2022Quote: ... The total volume was set to 65 μL and the reaction was carried out at room temperature in reaction tubes (Low Protein Binding, Sarstedt). For the control experiments ...
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bioRxiv - Cell Biology 2019Quote: ... Analysis of localization and expression of targeted proteins in clones were performed after 14- to 20-day period post transfection on a 24-well lumox (Sarstedt) plates ...
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bioRxiv - Microbiology 2022Quote: ... 10 µg total hNF protein (in 100 µl PBS) was immobilized on a 96-well polystyrene plate (Sarstedt REF:82.1581.001) by centrifugation (250 x g ...
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bioRxiv - Plant Biology 2019Quote: ... ground plant material was extracted in a 1-ml extraction mixture of chloroform, methanol and water (1:3:1, v:v:v) in Sarstedts Eppendorf tubes (Sarstedt AG & Co, Nümbrecht, Germany). One metal bead (a 3-mm tungsten carbide bead ...
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bioRxiv - Physiology 2022Quote: ... containing 30 µg protein in a total volume of 94 µl was loaded onto a black 96-well plate (Sarstedt, Nümbrecht, Germany). Two µl 50 mM ATP-Mg2+ (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... and manufacturer C (SafeSeal reaction tube no. 72.706 and SafeSeal reaction tube, Low protein-binding no. 72.706.600, Sarstedt AG & Co. KG, Nümbrecht, Germany). Further ...
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bioRxiv - Microbiology 2020Quote: ... The strains were then diluted 1:10 in Fraquil and 1 ml were distributed in 13-ml tubes (Sarstedt) and incubated in a 55 °C water bath ...
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bioRxiv - Cancer Biology 2023Quote: 1 x 105 cells were seeded in 24-well transwell inserts with 8 µm pore size (Sarstedt, 102511-1) in media containing 1% FBS ...
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bioRxiv - Plant Biology 2024Quote: Cubes (1×1×0.5cm) were cut from a petri dish with 25ml solid media using a cuvette (Sarstedt No.67.755). The cubes were then carefully weighted.
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bioRxiv - Immunology 2021Quote: PET membrane 1 μm transwell inserts (Sarstedt 83.3930.101) were placed with the bottom membrane facing upwards into 15 cm dishes ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin (Wisent) in T75 flasks (Sarstedt). Undifferentiated cells were passaged every 48-72 hours at semi-confluence ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM DTT) inside a 1 ml Polystyrene Cuvette (Sarstedt). MDH activity was quantified by measuring absorption at 340 nm every 10 s for 30 s on a Biochrom Novaspec Plus photometer ...
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bioRxiv - Immunology 2021Quote: ... 1×106 VeroE6 cells were seeded onto a T25 flask (Sarstedt) on the previous day DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: Caki-1 cells were seeded in clear-bottom Lumox plates (Sarstedt) one day prior to infection with EDB-2 at MOI = 1 ...
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bioRxiv - Cell Biology 2023Quote: ... 1×106 cells were seeded per well in 24-well plates (Sarstedt) one day before transfection ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL was dispensed into a 13 mL polypropylene culture tube (Sarstedt). Tubes of the treated lineage were immersed for 15 minutes in a pre-heated water bath set to the challenge temperature (55°C to 59°C) ...
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bioRxiv - Systems Biology 2023Quote: ... For each passage 1-2×106 cells were seeded in T25 flasks (Sarstedt). Then ...
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bioRxiv - Immunology 2023Quote: ... Cells (1 x 105/ml) were plated in a 48-well plate (Sarstedt, 83.3923) precoated overnight at 4 °C with 1 µg/ml anti-CD3χ and 3 µg/ml anti-CD28 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 1 ml blood was collected in EDTA-coated tubes on ice (Sarstedt, Numbrecht, Germany), centrifuged at 4°C (2000xg ...
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bioRxiv - Microbiology 2020Quote: ... 1 ml of cells were seeded in each well of the 24-well plate (Sarstedt).
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bioRxiv - Cell Biology 2023Quote: ... or 1:20 for maintenance in standard 100 mm culture dishes (#83.3902; Sarstedt, Nümbrecht, Germany). Differentiation of the myoblasts into myotubes was induced by replacing the growth medium with a low-mitotic medium i.e. ...
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bioRxiv - Immunology 2023Quote: ... 500µl of 1 x 106 mast cells/ml were added to 24 well plates (Sarstedt) and infected with 25µl inoculum ...
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bioRxiv - Bioengineering 2021Quote: ... Aliquots of 1 mL of the neutralized solution were cast in 48-well plates (Sarstedt, Germany) and kept at 37 °C for 30 minutes to undergo fibrillogenesis to form collagen hydrogels ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The THP-1 monocytes (106 cells/ml) were plated in a 100 mm Petri dish (Sarstedt) and treated with 100μM PMA for 24 h ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis seedlings were cultured in liquid medium (1 ml/well) in sterile 24-well plates (Sarstedt), essentially as described (30) ...
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bioRxiv - Immunology 2022Quote: ... 1 mL supplemented DMEM was added and the remaining cells were obtained using a cell scraper (Sarstedt). Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were activated overnight in a 48-well plate (1×106 cells/well) (Sarstedt) in T cell medium in the presence of plate bound anti-CD3ε (1 μg/ml ...