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Citations for Sarstedt :
51 - 100 of 364 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cell debris was removed by centrifugation at 12,000 × g for 15 min at 4°C and the supernatant filtered through a 0.45 μm pore size Filtropur S membrane (Sarstedt). The cleared extract was directly loaded onto a HisTrap FF crude column (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: Mouse blood was collected isolated using Microvette CV 300 Z serum separation tubes (SARSTEDT Germany) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2024Quote: ... adherent cells were collected with a cell scraper (Sarstedt, 83.1380). The cells/organoids were lysed in RLT lysis buffer (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: Barcoded Asaia bacteria were grown overnight at 30°C to early log phase (OD600 0.5-0.8) in a deep-well plate (Sarstedt, Nümbrecht, Germany) in 300 μl GLY medium supplemented with 100 μg/ml kanamycin per well ...
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bioRxiv - Microbiology 2022Quote: ... GG770 and LL1021 cells were grown aerobically at 37 °C under agitation in 5 ml of M9 minimal medium in 50 ml conical polypropylene tubes (Sarstedt) with an inclination of 90° ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were recovered for 5 hours at 30°C in YP medium with 2% maltose and then spread onto 50 petri dishes (150×20 mm, Sarstedt) with YNB + CSM Ura− with 2% maltose supplemented with G418 at 0.2 mg mL−1 ...
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bioRxiv - Microbiology 2019Quote: ... Cultures were inoculated to an initial OD600 of 0.05 into fresh medium from overnight liquid cultures grown in LB in 18mm borosilicate culture tubes at 37°C on a roller drum in 15 mL conical screw-cap tubes (Sarstedt). Tubes were placed in 2.5 liter AnaeroPack System rectangular jars (Mitsubishi Gas Chemical Co. ...
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bioRxiv - Neuroscience 2023Quote: ... Samples of each peptide and the peptide mix were incubated at 37 °C in sealed 2 ml non-treated plastic cryotubes (Sarstedt) for 24 hours and amyloid content was determined by ThT fluorescence and TEM (data not shown).
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bioRxiv - Cell Biology 2020Quote: ... in T25 polystyrene cell culture flasks for suspension cells (Sarstedt, Austria) at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by rapid scraping of cells using a cell scraper (Sarstedt) and collected into 2ml Eppendorf tubes ...
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bioRxiv - Cell Biology 2021Quote: Mouse blood was collected by cardiac puncture and transferred into microvette serum gel tubes (20.1344, Sarstedt). Samples were then centrifuged at 10,000 g for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse blood samples were collected with heparin-coated blood collection tubes (Sarstedt Microvette; CB 300 LH). The collected blood samples were centrifuged at 3000 g for 3 min ...
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bioRxiv - Biophysics 2020Quote: ... cells were seeded at the density of 3000 cells/cm2 in cell culture dishes with Ø = 35 mm (Sarstedt). They were kept in DMEM medium containing no phenol red (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: Adult flies of the desired genotype were collected and aged for 6 days at 25°C and transferred into 1% agar (in dH2O) contained in 12 mL vials (SARSTEDT, 58.487). Dead animals were counted in 15 ...
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bioRxiv - Microbiology 2019Quote: The level of pyoverdine and pyocyanine production was analyzed on a 72h-biofilm formed on Nephrophane or selected PAO1 mutants cultured (48 h, 37°C) in TSB medium (bioMerieux, France) in a 24-well polystyrene plate (Sarstedt, Germany) (Danis-Wlodarczyk et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was clarified at 45’000 g at 4°C for 45 min and the supernatant was filtered through a 0.4 µm syringe tip filter (Sarstedt, Nümbrecht, Germany). Lysate was loaded onto a pre-equilibrated 1 mL HisTrap FF column and washed with 12 % Buffer B (50 mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... D and E) or as catheter urine from a fresh collection bag (timepoint B and C) into standard urine sample cups (Sarstedt, Germany). Preservatives and protease inhibitors were added to the final concentrations 6,6mM NaN3 ...
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bioRxiv - Immunology 2022Quote: ... the unstressed sample was prepared once weekly and stored at 5 °C in 5 mL vials (#60.558.001, 57×15,3mm, Sarstedt AG & Co., Nümbrecht, Germany). The sample was used for two rounds of injections and hereafter discarded.
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bioRxiv - Microbiology 2023Quote: ... where a large volume of passage one stock was made and stored at -80°C in 500 μL aliquots in 2 ml cryovials (72.694.406, Sarstedt Inc. (Nümbrecht, Germany)) until required ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial growth was determined after 24 h of incubation (37°C, 200 rpm) by measuring at OD600 using standard photometer cuvettes (Sarstedt, Germany). An identical protocol was applied for the 20 clinical NDM-producing isolates with the only modification of using a fixed concentration of 4 µg/ml of aztreonam.
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bioRxiv - Microbiology 2021Quote: ... HeLa cells grown in 8-well removable cell culture chambers (Sarstedt, Germany) or on coverslips were infected and treated as described above ...
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bioRxiv - Immunology 2022Quote: ... resistant cells were transferred to 48-well cell culture plate (Sarstedt; 83.3923), and further grown over 3-4 weeks into stable cell lines ...
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bioRxiv - Cell Biology 2020Quote: ... a cell scraper (Sarstedt) was used to collect the remaining cell content ...
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bioRxiv - Cell Biology 2023Quote: ... and cell scraper (Sarstedt). Cell counts of every treatment condition were determined and adjusted to the condition with the lowest count ...
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bioRxiv - Microbiology 2023Quote: ... Parents were instructed to collect infant faeces from diapers and freeze them immediately at –20◦C in their home freezer inside a cool transport container (Sarstedt, Hilden, Germany). Samples were transported to the laboratory ...
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bioRxiv - Neuroscience 2023Quote: We collected blood from ad-libitum fed 9 months-old mice through cardiac puncture and centrifugation at 2000 g for 5 minutes at 4 C° in heparinised tubes (Microvette CB 300; Sarstedt, Leicester, UK) for plasma collection ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: ... the organoid pieces were digested in 2 ml of CellLive™ Tissue Dissociation Solution (Singleron Biotechnologies, #1190062) at 37°C for 15 minutes in a 15-ml centrifuge tube (Sarstedt, #62.5544.003), with continuous agitation on a thermal shaker ...
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bioRxiv - Biochemistry 2023Quote: ... and manufacturer C (SafeSeal reaction tube no. 72.706 and SafeSeal reaction tube, Low protein-binding no. 72.706.600, Sarstedt AG & Co. KG, Nümbrecht, Germany). Further ...
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bioRxiv - Neuroscience 2023Quote: ... mouse blood was collected by cheek puncture into 1.1-ml z-gel serum collection tubes (Sarstedt; Germany) and stored on ice in the dark at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... All mouse blood samples were collected from the tail using Microvette capillary tubes (Sarstedt Inc, Cat# NC9141704). Fasting plasma insulin levels were measured using the Mouse Insulin ELISA Kit (Proteintech ...
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bioRxiv - Immunology 2021Quote: ... The cell suspensions were filtered through a 70 µm cell strainer (Sarstedt #83.3945.070) and incubated for 30 min at 4°C with fixable viability dye eFluor455UV (eBioscience #65-0868 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells from a confluent 6 cm plate (about 2.7*106 cells, 83.3901.300, Sarstedt) were harvested with 300 µl of boiling 1x SDS loading dye (3 % (w/v ...
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bioRxiv - Cell Biology 2023Quote: TIFF cells were seeded on 10 cm diameter cell culture dishes (Sarstedt, #83.3902) and treated as described above for CDM growth ...
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bioRxiv - Microbiology 2019Quote: ... and used to inoculate 200 µL of M9 medium supplemented with the desired C-source in a 96-well microtiter plate (Falcon, product no. 353047 or Sarstedt, product no. 83.3924) and incubated at 30°C and 250 rpm in a microplate reader (Xenius ...
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bioRxiv - Genetics 2022Quote: ... Blood was collected by venous puncture or cardiac puncture at the time of sacrifice and centrifuged at 2000 g for 15 minutes at 4 °C in heparin-coated tubes (Sarstedt AG, Nürnbrecht, Germany) to separate plasma from cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated sample was centrifuged (15000 x g, 30 min, 4 °C) and the supernatants were transferred into fresh DNA-low binding tubes (Sarstedt, Cat.-No. 72.706.700). The protein concentration was measured using Bradford assay (Roti®-Quant ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded at a density of 400 cells/mm2 or 300 cells/mm2 in 60 mm dishes (Sarstedt, North Rhine-Westphalia, Germany) and allowed to grow to 80-90% confluency ...
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bioRxiv - Microbiology 2020Quote: ... vermiformis cells were grown at room temperature in 75 cm2 cell culture flasks (Sarstedt) in modified PYNFH medium (ATCC medium 1034 ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 14,000 cells/cm2 iAF cells were seeded onto 12-well plates (Sarstedt, 83.3921.005) and in separate cultures approximately 14,000 cells/cm2 NP cells were seeded onto hanging inserts (Corning Costar 0.2 µm pore PTFE transwells) ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were seeded at 4·106 cells per 100 mm polystyrene dish (Sarstedt) 1 day before transfection in order to achieve 80% confluency at the moment of transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... standard for adherent cells (Sarstedt). Culture medium was changed every 2-3 days upon reaching around 80% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 75,000 cells/well HeLa cells were seeded on 10 cm dishes (Sarstedt) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were scraped off the cell culture dish surface with a cell scraper (Sarstedt, #83.1830) and the matrix was transferred into 1.5 ml centrifugation tubes.
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: Fecal pellets were collected fresh from each mouse in a cage directly into a screw cap micro tube (Sarstedt) at the indicated times ...
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bioRxiv - Immunology 2021Quote: ... Mouse peripheral blood was taken from the lateral tail vein with an EDTA coated Microvette 100 K3E (Sarstedt, 20.1278). Full blood counts were measured on an IDEXX ProCyte Dx™ Hematology Analyzer (IDEXX Laboratories) ...
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bioRxiv - Systems Biology 2020Quote: ... The kit contains a 1.5mL screw cap tube (Sarstedt) containing 200uL of RNA preservative buffer (RPB) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were trypsinized and seeded onto a 96-well standard cell culture plate (Sarstedt, Nümbrecht, Germany). Cell seeding was then performed done by pipetting 100 μl each with 10,000 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were trypsinized and seeded in a 96-well standard cell culture plate (Sarstedt, Nümbrecht, Germany). Cell seeding was then performed done by pipetting 100 μl of RPMI1640 with 10% FCS with 75,000 cells per well ...
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bioRxiv - Neuroscience 2020Quote: Cells were seeded onto poly-L-lysine precoated chamber slides (5000 cells/well; Sarstedt, Nümbrecht, Germany). Briefly ...