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Citations for American BioInnovations, :
101 - 150 of 154 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... B2M and 18s rRNA transcripts was performed using Amplitaq Gold SYBR Master mix (ABI) on Applied Biosytems step one plus real-time thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... The real-time PCR was performed by using SYBR Select Master Mix (ABI, USA), and 16S rDNA was used as reference gene to normalize data ...
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bioRxiv - Biochemistry 2024Quote: ... qRT reaction was carried out using and Sybr Green master mix (ABI Biosystems #4309155) (Varghese et al. ...
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bioRxiv - Microbiology 2023Quote: Quantitative real-time PCR was performed using the SYBR Green PCR master-mix (ABI) on a QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: Quantitative real-time RT-PCR (qRT-PCR) was performed using 7500 Real Time PCR system (ABI) in 20 μl reactions using AgPath-ID One-Step RT-PCR Reagents 10 μl RT-PCR buffer (2X ...
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bioRxiv - Developmental Biology 2020Quote: ... Real-time RT-PCR was performed in a StepOne plus system (ABI StepOne Plus, ThermoFisher, USA) using SYBR green master mix (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Tm was measured using a ramp from 25°C to 95C on ViiA7 qPCR machine (ABI) over 30min.
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bioRxiv - Microbiology 2020Quote: ... Low ROX (QuantaBio)’ master mix on an ABI 7500 Fast Real-Time PCR System (ABI).
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bioRxiv - Genomics 2019Quote: ... RT-PCR products were accurately sized and peak areas calculated (Relative Fluorescence Units – RFUs) using GeneMapper (ABI) software.
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed with the ABI Step one plus Real- Time PCR (RT-PCR) system (ABI, USA) using One Step TB Green(tm ...
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bioRxiv - Neuroscience 2021Quote: ... The protocol followed the manufacturer’s recommendations with the exception of using 2 μl for each RT primer (ABI) in a 10 μl total reaction volume (i.e. ...
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bioRxiv - Neuroscience 2020Quote: ... it was more accurately quantified using qPCR with the Step One Plus Real-Time PCR system (ABI, USA).
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bioRxiv - Genetics 2020Quote: ... the qualified insert size was accurately quantified using qPCR with the StepOnePlus Real-Time PCR system (ABI, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System (Illumina ...
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bioRxiv - Genomics 2020Quote: ... the purified DNA was quantified with ABI ViiA 7 and Power SYBR Green Master Mix (ABI 4368706) and normalized by genomic DNA ...
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bioRxiv - Microbiology 2019Quote: ... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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bioRxiv - Molecular Biology 2019Quote: ... Power SYBR Green PCR Master Mix (Fermentas, Canada) was used in the qRT-PCR analyses (ABI-7500FAST). The relative mRNA level was calculated based on the average Ct value of the target gene and the Alu reference [2-(Cttarget_gene-CtAlu)] [49].
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bioRxiv - Neuroscience 2021Quote: ... We then amplified the cDNA by real-time PCR using TaqMan Fast Advanced Master Mix (2X, ABI) and mRNA-specific TaqMan assays (20X ...
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bioRxiv - Microbiology 2019Quote: ... All qRT-PCR experiments were performed using Thermo Fisher’s Power Sybr Green PCR Master Mix (4367659; ABI) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PowerUp SYBR Green Master Mix was then used for qRT-PCR reactions using Second Step instrument (ABI). Transcriptional levels of genes were normalized to 16S rRNA ...
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bioRxiv - Microbiology 2023Quote: ... PowerUp SYBR Green Master Mix was then used for qRT-PCR reactions using Second Step instrument (ABI). Transcriptional levels of genes were normalized to GAPDH.
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bioRxiv - Biophysics 2019Quote: ... Heterozygous adults and mutant larvae were genotyped using a KASP assay (LGC genomics) on a qPCR machine (ABI StepOne). All procedures were performed on zebrafish embryos in accordance with the European Communities Council Directive and the Norwegian Food Safety Authorities ...
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bioRxiv - Microbiology 2020Quote: ... A new probe that would complement the primers and be compatible with TaqMan qPCR requirements (ABI 7700 Users Manual) was designed by using Integrated DNA Technologies (IDT ...
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bioRxiv - Microbiology 2021Quote: ... They were quantitated by quantitative polymerase chain reaction (qPCR) using a Roche KAPA Library Quantification Complete Kit (ABI Prism), and run on the Applied Biosystems QuantStudio 5 Real-Time PCR System.
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bioRxiv - Developmental Biology 2022Quote: ... qPCR was run in 384-well plates on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (ABI) and analyzed in Microsoft Excel.
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bioRxiv - Immunology 2023Quote: ... PCR amplification was performed by using RT-PCR in the presence of SYBR green in a 7500 Fast Realtime PCR System (ABI). Specific primers were designed with Primer Express 2.0 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was conducted using designed primers with the primerdb database and PowerUp SYBR Green Master Mix (ABI) in QuantaStudio (ABI) ...
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bioRxiv - Immunology 2023Quote: ... 2 μl of cDNA were added to 23 μl of PCR mixture containing 2xSYBR Green Master Mix (ABI) and 0.2 μM of forward and reverse primers ...
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bioRxiv - Biochemistry 2021Quote: ... Realtime quantitative PCR (qPCR) was performed using the SYBR Green method and cycle threshold values (Ct) were obtained using an Applied Biosystem (ABI) 7500 Real-Time PCR machine and normalized to GAPDH.
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bioRxiv - Neuroscience 2021Quote: ... We performed qPCR using 1 μL of cDNA with 7.60 µl of Taqman Universal PCR Master Mix (with No Amperase UNG, ABI) and 0.75 µl of miRNA-specific PCR TaqMan MicroRNA probes (20X ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was tested by semi-quantitative real time reverse transcription polymerase chain reaction (RT-PCR) on the StepOnePlus platform (ABI, Beverly, MA) with qScript XLT 1-Step RT-PCR ToughMix ...
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bioRxiv - Neuroscience 2021Quote: ... Gene expression analyses were performed on 12 ng cDNA per reaction using the TaqMan Fast Advanced Master Mix (ABI, 4364103) in a 384 well plate on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... reactions were carried out in biological triplicate with QuantiTect SYBR Green Master Mix using an Applied Biosystems StepOne Plus real-time machine (ABI). Results were analyzed using the ΔΔCT method ...
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bioRxiv - Cell Biology 2021Quote: ... All real-time PCR was carried out with Power SYBR Green PCR master mix on the ABI prism 7900 HT sequence detection system (ABI) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The template concentration was 10-15 ng while the concentration of forward and reverse primer was 10 ng in SYBR select Master Mix (ABI). The PCR was performed using the ABI 7700 sequence detector (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: The qPCR reactions were performed with samples in triplicate on an ABI 7500 fast real-time PCR system using power SYBR green PCR master mix (ABI). The mouse TRPV1 cDNA was amplified with primers 5’-TTCCTGCAGAAGAGCAAGAAGC-3’ and 5’-CCCATTGTGCAGATTGAGCAT-3’ (Albers et al. ...
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bioRxiv - Immunology 2020Quote: ... Quantitative RT-PCR was performed on cDNA using either TaqMan primers and probes or QuantiTect primers in combination with TaqMan PCR Master Mix (ABI) or SYBR Green chemistry and reactions were run on a RT-PCR system (QuantiStudio 6 Flex ...
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bioRxiv - Cell Biology 2022Quote: ... qRT-PCR reactions were performed with the TOYOBO SYBR Green Realtime PCR Master Mix (TOYOBO) and analyzed with a step-one Plus PCR system (ABI). Lotus Ubiquitin (Lj5g3v2060710.1 ...
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bioRxiv - Plant Biology 2023Quote: ... by using the HieffTM qPCR SYBR Green Master Mix (Low Rox Plus, cat no. 11202ES08, Yeasen, Shanghai, China) on a QuantStudio 6 Flex PCR (ABI). The qPCR signals were normalized to those of the reference gene PST in pine trees ...
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bioRxiv - Neuroscience 2023Quote: ... Messenger RNA levels were quantified by using the StepOnePlus Real-Time PCR System with Fast SYBR Green Master Mix (ABI). rp49 was used as internal control ...
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bioRxiv - Microbiology 2023Quote: ... was performed using SYBR Green Real-time PCR Master Mix (QPK-201, Toyobo) with the QuantStudio 6 Flex multicolor real-time PCR detection system (ABI). Relative mRNA levels were normalized to GAPDH levels and calculated using the 2-ΔΔCT method (Livak & Schmittgen ...
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bioRxiv - Plant Biology 2024Quote: ... with Hieff™ qPCR SYBR Green Master Mix (Low Rox Plus, cat no. 11202ES08, Yeasen, Shanghai, China) on a QuantStudio 6 Flex PCR system (ABI). The qPCR signals were normalized to those of the reference gene PST in Pinus by applying the 2-ΔΔCT method (Fang et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out in technical triplicate with QuantiTect SYBR Green Master Mix using Applied Biosystems StepOne Plus real-time machine (ABI). Results were analyzed using ΔΔCT method ...
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bioRxiv - Genomics 2020Quote: The purified cDNA or dsDNA samples were assayed by quantitative PCR with ABI ViiA 7 and Power SYBR Green Master Mix (ABI 4368706) using custom designed primers (Additional file 3 ...
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bioRxiv - Microbiology 2021Quote: ... was amplified and detected by SYBR Green PCR Master Mix kit (bao bio-engineering Co., Ltd, China) on the ABI7500 instrument (ABI, USA). For qRT-PCR reactions ...
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR reactions using the PowerUp™ SYBR Green Master Mix were carried out on the QuantStudio 12K Flex Real-Time PCR System (ABI) (ABI ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95°C for 15 seconds and 60°C for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Neuroscience 2019Quote: ... with ten-fold dilution of cDNA and 200 nM of each primer using the SYBR Select PCR Master Mix (ABI, Thermo Fisher). Primers are listed in Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR reactions using the PowerUp™ SYBR Green Master Mix were carried out on the QuantStudio 12K Flex Real-Time PCR System (ABI) (ABI, A25742). Data were analyzed by the 2−ΔΔCT relative quantification method ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95 C for 15 seconds and 60 ℃ for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...