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Citations for American BioInnovations, :
251 - 300 of 302 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95 C for 15 seconds and 60 ℃ for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Neuroscience 2020Quote: ... High Capacity cDNA Reverse Transcription Kit (#4368814) was from ABI/Thermo (Waltham ...
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bioRxiv - Immunology 2022Quote: ... The hypervariable region V3-V4 of the bacterial 16S rRNA gene was amplified with primer pairs 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’) by an ABI Gene Amp® 9700 PCR thermocycler (ABI, CA, USA). The PCR reaction mixture included 4 μL 5× Fast Pfu buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using a QuantStudio™ 7 Flex device and results were analyzed with QuantStudio™ Real-Time PCR software (both ABI, Waltham, MA, USA)
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bioRxiv - Cancer Biology 2019Quote: ... with 0.1 µM of each primer and 20 ng of genomic DNA using an Applied Biosystems 7500 real-time PCR instrument (ABI 7500; Thermo Fisher Scientific). PCR conditions were as follows ...
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bioRxiv - Genomics 2022Quote: ... and gene expressions of apoLp and POA3-like after male feeding on dsRNAapoLp were evaluated by qRT-PCR analysis (ABI StepOne Plus, Foster, CA) using TB Green Premix Ex Taq II (Catalog No ...
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bioRxiv - Genomics 2022Quote: ... was used for RSV subtype detection with TaqPath 1-Step Multiplex Master Mix (No ROX) on Applied Biosystems 7500 Real-Time PCR System (ABI, Life Technologies, Waltham, MA) as instructed ...
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bioRxiv - Cancer Biology 2024Quote: ... the libraries were quantified (KAPA Library Quantification Kit (Illumina/ABI Prism), normalized ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor necrosis factor α (TNF-α) and TATA box binding protein (TBP) were determined by real☐time PCR (ABI 7900 Prism, Applied Biosystems, US). Sequences used to amplify a fragment of IL-6 were FP ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed using TAQMAN gene expression master mix of equivalent amounts of total cDNA for forty cycles (ABI GeneAmp 9700 DNA thermal cycler). Endpoint data was assembled by comparison of Delta-Ct values for HN1 versus corresponding GAPDH Delta-Ct values for each cell line ...
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bioRxiv - Neuroscience 2020Quote: ... We used the High Capacity cDNA Reverse Transcription Kit (#4368814) from ABI/Thermo Fisher per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... cDNA was synthesised using the High Capacity RNA-to-cDNA kit (ABI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription was performed using a highcapacity RNA-cDNA kit (Applied Biosystems [ABI]) with 1 μg RNA per 20 μL reaction ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (ABI, # 4368814).
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was produced using the High-Capacity cDNA Reverse Transcription kit (ABI) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (ABI). Power SYBR Master Mix (ABI ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was prepared using 10 µL RNA (High Capacity cDNA Reverse Transcription kit, ABI). The Light Cycler 480 probe master kit (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription reaction was carried out using High Capacity cDNA Reverse Transcription Kit (ABI), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and Ex-protein fractions using TaqMan microRNA Reverse transcription kit (ABI 4366597 Lot#00636931), TaqMan miRNA stem-loop RT primers/qPCR primer probe set (ABI 4427975) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was carried out using High-Capacity cDNA Reverse Transcription Kit (ABI, 4368814) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and RISC-Poor (36-47) using TaqMan microRNA Reverse transcription kit (ABI 4366597 Lot#00636931), TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975 ...
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bioRxiv - Neuroscience 2021Quote: ... for reverse transcription with the High-Capacity cDNA Reverse Transcription Kits (ABI Applied Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, ABI 4368814). PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and reverse transcription was performed using a high-capacity RNA-cDNA kit (Applied Biosystems [ABI]) with 1 μg RNA per 20 μl reaction ...
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bioRxiv - Immunology 2019Quote: ... or whole blood (PAXgene Blood RNA Kit) and reverse-transcribed (ABI High Capacity Reverse Transcriptase). The expression of ISGs (IFIT1 ...
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bioRxiv - Immunology 2020Quote: ... cDNA was obtained by reverse transcription using the High-capacity cDNA Reverse Transcription Kits (ABI). Samples were analyzed by real-time PCR with GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Immunology 2021Quote: ... cDNA was obtained by reverse transcription using the High-capacity cDNA Reverse Transcription Kits (ABI). Samples were analyzed by real-time PCR with LightCycler Taqman Master (Roche ...
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bioRxiv - Molecular Biology 2020Quote: The sequencing reaction was carried out using Big dye Terminator v3.1 cycle sequencing kit (ABI, 4337454) in 10μl volume (containing 0.5μl purified DNA ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was generated in 20 µl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Quantitative real time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using the high-capacity reverse transcription kit (ABI) according to the manufactures’ instructions ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription was performed using the Taqman® Reverse Transcription kit (Life Technologies/ABI, Rotkreuz, CH). Real-time PCR was performed using presynthesized Taqman®-based Assays-on-Demand (Life Technologies/ABI ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg total RNA was reverse transcribed using the cDNA archive kit (Applied Biosystems – ABI, USA) following manufacturer’s instructions and the resulting cDNA was used as a template for Real Time PCR analysis ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was generated with random oligomer primers by using cDNA reverse transcription kit (ABI Biosystems #4368814). qRT reaction was carried out using and Sybr Green master mix (ABI Biosystems #4309155 ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed with the High-Capacity cDNA Reverse Transcription Kit (ABI, New York, NY, USA). Messenger RNA levels were quantified by using the StepOnePlus Real-Time PCR System with Fast SYBR Green Master Mix (ABI) ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was synthesized using 500-1000 ng RNA and the High-capacity cDNA reverse transcription kit (ABI) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription was conducted at 50°C for 15 min using the High Capacity Reverse Transcription kit (ABI) and 10-50 ng total RNA ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were titered by measuring p24gag levels with an HIV-1 p24 antigen capture assay kit (ABI#5447), and also by TZM-bl assay.
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bioRxiv - Microbiology 2019Quote: We adapted the AFLP protocol of the Applied Biosystems Microbial Fingerprinting Kit (Applied Biosystems [ABI], Foster City, CA, USA) for use with our samples ...
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bioRxiv - Microbiology 2021Quote: ... They were quantitated by quantitative polymerase chain reaction (qPCR) using a Roche KAPA Library Quantification Complete Kit (ABI Prism), and run on the Applied Biosystems QuantStudio 5 Real-Time PCR System.
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was synthesized by reverse transcription of 400 ng of RNA using the High Capacity cDNA Transcription Kit (ABI) with random primers ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 500ng RNA was reverse transcribed to cDNA using the ABI high-capacity cDNA archive kit (ABI # 4322171) as per the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... The DNA sequence of the cloned devil CIITA transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI), Foster City ...
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bioRxiv - Neuroscience 2020Quote: ... 1μg total RNA was used to synthesize cDNA using the High Capacity c-DNA Reverse Transcription kit (ABI-P/N 4368814). cDNA was diluted 1 ...
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bioRxiv - Neuroscience 2022Quote: ... was converted to complementary DNA (cDNA) using the High Capacity cDNA Reverse Transcription kit (Catalog number: 468814, Applied Biosystems Inc. (ABI), Foster City ...
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bioRxiv - Immunology 2020Quote: ... The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI), Foster City ...
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bioRxiv - Neuroscience 2019Quote: ... Two micrograms of total RNA from each sample was used for first-strand cDNA synthesis using the Retroscript Kit (Applied Biosystems, ABI). The reverse transcriptase was omitted in the negative control groups for qPCR.
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bioRxiv - Developmental Biology 2023Quote: ... Real-time PCR was performed by using a SYBR Premix Ex Taq kit (Q711-02, Vazyme) on the QuantStudio 5 Real-Time System (ABI, USA). The data were normalized against the levels of Rpl7 expression ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA using the High-Capacity cDNA Reverse Transcription Kit (ABI, Thermo Fisher Scientific) and random hexamers ...