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Citations for Tocris :
451 - 500 of 509 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 1 uM of the microtubule-stabilizing agent SAHA (TOCRIS bioscience Cat. No. 149647-78-9) for 30 minutes before activation or experimental procedures ...
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bioRxiv - Genetics 2023Quote: ... cells underwent various drug experiments: for cAMP analogs (8Br-cAMP, db-cAMP, and 6Bnz-cAMP, Tocris Bioscience, Bristol, UK), they were incubated for 48 hours with the appropriate concentrations (50 and 500µM for 8Br-cAMP and db-cAMP ...
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bioRxiv - Neuroscience 2024Quote: ... mEPSC recordings were obtained from cells voltage-clamped at –70 mV in ACSF containing 0.5 µM tetrodotoxin (TTX, Tocris) and 50 µM picrotoxin (PTX ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Neuroscience 2019Quote: ... The chemical inhibition of CTL6 cells was conducted by a global perfusion of clozapine-n-oxide (CNO, 5uM, Cat# 4936, Tocris), the chemical actuator of the chemogenetic probe ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Cell Biology 2021Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were either untreated (GFP-Apex and S1R-Apex) or treated (S1R-Apex) with 25μM Haloperidol (Tocris, Minneapolis, MN) or 20μM (+)-PTZ (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were rinsed with ice cold PBS and then collected and suspended by scraping in PBS containing 50nM JZL195 (Tocris), a dual inhibitor of FAAH and MAGL (Long et al. ...
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Immunology 2019Quote: ... To test the effect of VIP on ILC3, cells were incubated with different concentrations of VIP (human, rat, mouse, rabbit, canine, porcine) from Tocris, Bay 55-9837 (Tocris) ...
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Genetics 2019Quote: ... cells were treated with phorbol 12-myristate 13-acetate (PMA; 100nM in DMSO) or DMSO or PKC antagonist (GF10930X; Tocris) for 24 hours following which cells were lysed for dual luciferase analysis as per manufacturer’s instructions (Promega UK).
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2022Quote: ... Then cells were hyperpolarized to -80 mV to isolate AMPA currents which were then were blocked by NBQX (20uM; Tocris) and finally the cells got depolarized to +40 mV to measure NMDA-mediated currents which were blocked by the selective NMDA antagonist AP-V (50 uM ...
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bioRxiv - Cell Biology 2022Quote: ... the actin depolymerizer latrunculin B (10 μM; for 30 min only to preserve cell viability; Tocris Bioscience; Thermo Fisher Scientific), or the YAP inhibitor verteporfin (0.5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Cancer Biology 2023Quote: ... we washed cells once with PBS and then added various concentrations of drugs (tamoxifen, fulvenstrant, or 18albociclib; all purchased from Tocris) in phenol red free DMEM (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in an ad hoc defined generic medium (Supplementary Table 2) supplemented with Rho-kinase inhibitor (ROCKi; Tocris) and Primocin ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were continuously perfused with this saline solution during whole-cell recording with the addition of 20 μM NBQX and 20 μM (R)-CPP (Tocris) to isolate inhibitory synaptic activity ...
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bioRxiv - Biochemistry 2022Quote: ... hiPSCs were patterned for 21 days with a growth factor cocktail to promote differentiation toward ventral midbrain neuronal progenitor cells for 11 days (10 mM SB431542, Tocris; 100 nM LDN193189 ...
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bioRxiv - Developmental Biology 2023Quote: ... Mechanically dissociated cells in accutase were diluted with 4x volume of StemFlex with 10uM Y-27632 (ROCK inhibitor, Tocris, #1254) and manually counted with Trypan Blue ...
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bioRxiv - Systems Biology 2023Quote: These stable cell lines were split into separate wells of a 24-well plate and either treated with doxycycline (Tocris) or left untreated until analyzed by flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with quercetin for approximately 24 hours with DMSO used as a negative control and ActD (Tocris 1229) used as a positive control ...
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bioRxiv - Cancer Biology 2023Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Immunology 2019Quote: ... Cell were then washed and rested overnight in complete RPMI medium without or with JAK kinase inhibitor CP-690550 (Tocris Biosciences), and stimulated for 4 hours with 100U/ml of IL-2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were whole-cell voltage-clamped at −40mV and recorded in the presence of the GABAAR antagonist Gabazine (10 μM, Tocris 1262). N-methyl-D-aspartic acid (NMDA ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were seeded on Matrigel-coated plates or coverslips at a density of 1.1×105 cells/cm² in mTeSR1 medium supplemented with 10 μM of ROCK inhibitor Y-27632 (Ri; Tocris, ref. 1254). To induce MES differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2022Quote: Macrophages were plated in a 12-well plate at 106 cells per well and treated with 10 μM E64 (Tocris, 5208) or 20 μM Z-Phe-Ala-FMK cathepsin B inhibitor (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...
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bioRxiv - Immunology 2020Quote: ... anti-IgM/CpG-activated or non-activated B cells were incubated with or without reserpine (5 μM, Tocris, catalogue number: 2747), an inhibitor of VMAT-1 & VMAT-2 for 45min ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Human ePSCs were thawed at 37°C and plated on top of the feeder cells in EPSCM with 10 µM Y-27632 dihydrochloride (Tocris, 1254) for 48 h ...
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Cancer Biology 2023Quote: ... 1000 cells/well were seeded and pre-treated with SCFAs (SP or SB) for 48 hours followed by cisplatin (Tocris, 2251) treatment in a dose dependent manner for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting after treatment of the cells with 1 µM dTAG-7 (Tocris, 6912) for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... 12-well plates at 7-8 x 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y- 27632; Tocris, 1254). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were then differentiated to neural precursor cells by dual SMAD inhibition using 0.1 mM LDN193189 (Biotechne/Tocris Cat. No. 6053) and 10 mM SB431542 (Biotechne/Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... then treated for an additional 24 h in the presence (synchronised in G2 cell cycle phase) or absence (asynchronised) of 9 μM CDK1 inhibitor RO-3306 (CDK1i; Tocris Bioscience) prior to collection.
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bioRxiv - Neuroscience 2023Quote: iPSCs were replated at a density of 2 x 104 cells/cm2 in mTeSR medium containing 10 µM Rho kinase inhibitor (Y-27632, Tocris #5849), which was removed after 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... all buffers and solutions used during the process (from animal perfusion to sorted cells flash freezing) were supplemented with a cocktail composed of Actinomycin D (3μM, Tocris #1229/10), Anisomycin (100 μM ...
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bioRxiv - Immunology 2024Quote: ... The following inhibitors were added to the cells for 24 hours before harvesting: the NF-κB inhibitor JSH23 (2 µM, Tocris Bioscience), STING inhibitor H151 (5 µM ...
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bioRxiv - Neuroscience 2022Quote: ... 200 nM, 90 min application for the experiments on transfected cells; 120 min application for electrophysiological recordings in oocytes, Tocris; Cat. #5756) [18 ...
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bioRxiv - Neuroscience 2021Quote: ... Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 µM, Tocris bioscience; 1265), NBQX (10 μM ...