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Citations for Tocris :
601 - 650 of 664 citations for Recombinant Human Interleukin 22 Receptor Alpha 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... organoids cultured for 8 days with airway cell culture medium were followed by inclusion of CHIR99021 (2 µM, Tocris) for additional 10 days with or without DAPT (20 µM) ...
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bioRxiv - Neuroscience 2020Quote: ... all electrophysiological recordings were performed in the presence of picrotoxin (100μM) and 3-((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP; 10μM; Tocris Bioscience), and a cut was made between CA3 and CA1 regions to prevent the propagation of epileptiform activity ...
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bioRxiv - Neuroscience 2023Quote: Pharmacological inhibition of AQP4 was achieved using TGN-020 (N-1,3,4-thiadiazol-2-yl-3-pyridinecarboxamide, Tocris Bioscience, UK). Unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... 3- (4,5-dimmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to estimate cell number following the manufacturer’s description (Tocris, # 5224), and absorbance was measured at 550 nm in an EnsightTM 96-well plate reader (Perkin Elmer).
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bioRxiv - Molecular Biology 2023Quote: ... BI stands for Broccoli aptamer ligand (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-di-fluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride) (Cat. No. 7466, Tocris) (14) ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated from the 100 mm dishes and 2.8-3M cells per well were seeded on the prepared 2-well dishes in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) media for 3h ...
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bioRxiv - Neuroscience 2021Quote: ... Four of the rats were also infused with 2 μL of either physiological saline or 5 μg/μL yohimbine (Tocris) with a flow rate of 0.5 μL/min as previously reported (Caetano et al. ...
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bioRxiv - Cell Biology 2019Quote: Human PSC were grown for 5 days in E8 media(Chen et al., 2011) without FGF-2 and TGF-b but supplemented with 10μM CHIR99021 (Tocris, 4423). Loss of pluripotency was confirmed by RT-qPCR panel of self-renewal ...
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bioRxiv - Genomics 2020Quote: ... The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience, UK). The used concentrations of inhibitors were selected based on recommendations provided by the manufacturers ...
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bioRxiv - Neuroscience 2020Quote: ... and the Mai Tai eHP was tuned to 725nm for photoactivation of recirculated caged MNI-L-glutamate (2 mM, Tocris)57,58 ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Neuroscience 2021Quote: ... mEPSCs were recorded in aCSF supplemented with 10 μM N-[3-[4-(3-Aminopropylamino)butylamino]propyl]-2-naphthalen-1-ylacetamide trihydrochloride (NASPM, Tocris) to selectively block CP-AMPARs ...
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bioRxiv - Neuroscience 2020Quote: ... QX-314 (Cat No. 1014); and D-2-amino-5-phosphonovalerate (D-AP5; Cat No. 0106) were purchased from Tocris.
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bioRxiv - Neuroscience 2020Quote: ... The GAT1 blocker NO-711 [(1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene)amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride] and GAT3 blocker SNAP-5114 [1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid] were purchased from Tocris Bioscience (Minneapolis ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazoledihydro-chloride (MPP; cat# 1991) were purchased from Tocris Bioscience ...
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bioRxiv - Neuroscience 2021Quote: ... The mGluR5 specific negative allosteric modulator MTEP (3-((2-Methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride) was purchased from Tocris (2921) and administered to brain slices at 1 μM by bath perfusion ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 6 hrs (Figure 6 – figure supplement 2) or 24 hrs after transfection (the remaining experiments) neurons were treated with tetrodotoxin (TTX, 5 µM, Tocris) for ∼16 hours ...
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bioRxiv - Neuroscience 2019Quote: ... 2-(3,4-Dichlorophenyl)-N-methyl-N-[(1S)-1-(3-isothiocyanatophenyl)-2-(1-pyrrolidinyl)ethyl]acetamide (DIPPA) HCl (10 mg/kg Tocris) dissolved in 10% DMSO.
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bioRxiv - Neuroscience 2019Quote: ... Specific blockade of GABA-B receptors was performed by wash in of 10 µM CGP 55845 hydrochlorid [(2S)-3-[[(1S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid hydrochloride] (Tocris Bioscience) for 20 minutes.
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2020Quote: The FPR2 agonist Compound-43 (N-(4-Chlorophenyl)-N-[2,3-dihydro-1-methyl-5-(1-methylethyl)-3-oxo-2-phenyl-1H-pyrazol-4-yl]-urea) and antagonist WRW4 (Trp-Arg-Trp-Trp-Trp-NH2) were purchased from Tocris Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... During BQA experiments the ACSF solution contained the following substances: 10 μM D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5) (Tocris), 10 μM 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX ...
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bioRxiv - Microbiology 2022Quote: ... 3 μl of 10 mM of NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Tocris Bioscience) was added to the resuspension ...
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bioRxiv - Cell Biology 2022Quote: Compounds which were considered a hit after library screening were purchased from the following commercial suppliers: 2-TEDC (Tocris Bioscience), Anethole-trithione (ATT ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053] ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614] ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in an ad hoc defined generic medium (Supplementary Table 2) supplemented with Rho-kinase inhibitor (ROCKi; Tocris) and Primocin ...
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bioRxiv - Neuroscience 2023Quote: ... and (3-chloro-4-fluoro-N-[(4-([2-(phenylcarbonyl)hydrazino]carbonyl) phenyl)methyl]benzene-sulfonamide) (TCN-201) (Hansen et al., 2012)were obtained from Tocris Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... 1 MgCl2, and 2 CaCl2 (300-310 mOsm, pH adjusted to 7.4 with NaOH) supplemented by SC79 (5uM, Tocris 4635) or A769662 (1uM ...
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bioRxiv - Biochemistry 2023Quote: ... Washed membranes were incubated in the hypotonic buffer in the presence of 2 mg/ml iodoacetamide and 50 μm SSR149415 (Tocris) for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2- yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate (NR12S, Cat No 7509, Tocris) was used to measure membrane fluidity ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 25 µl PBS together with 0.25 µl of 5 mM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl) carbonyl]amino]-D-alanine hydrocholoride (HADA) (Tocris) dissolved in DMSO ...
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bioRxiv - Microbiology 2024Quote: ... the cells were hybridized with V-EV71-C1 probe (Biotechne) at 40°C for 2 hours and then the signals were revealed using TSA Vivid 570 kit (Tocris). The slides were then incubated with blocking buffer and incubated with MAB979 (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... NMDA receptor (R) dependency was tested by application of an NMDAR antagonist (APV 50 µM, DL-2-Amino-5-phosphonopentanoic acid, Tocris, Germany) in the bath solution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a fourth group (DZP) received 2 mg/kg diazepam (CAS #439-14-5; Tocris, 2805; German-Ponciano et al., 2020) as a pharmacological control of anxiolytic activity ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was then changed to 3N (50:50 DMEM/F12:neurobasal with N2 and B27 supplements) (Shi et al., 2012) without vitamin A with 2 µM DMH1 (Tocris, 4126), 2 µM XAV939 (Cayman Chemical ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 50 μM D,L-2-amino-5-phosphonovaleric acid (AP5) (both from Tocris, Bristol, UK) to block recurrent glutamatergic signaling.
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bioRxiv - Neuroscience 2020Quote: ... to isolate inner retinal signaling (B-waves) and DL-2-Amino-4-phosphonobutyric acid (DL-AP4, 40µM, Tocris Bioscience, Bristol, U.K.) to isolate the photoreceptor component of the ERG signal (A-wave) ...
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bioRxiv - Systems Biology 2020Quote: ... On Day 3 we changed the medium to RPMI + 2% B-27 (minus insulin) + 1uM Xav 939 (Tocris Bioscience, cat. 3748) and incubated the cells for 48 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... From day 2 to day 4 the medium was additionally supplemented with BMP and TGFβ inhibitors (Noggin, Peprotech and SB431542, Tocris Bioscience). Treatment with MEK inhibitor (PD184352 ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors were resuspended in DMSO and serially diluted in medium to their final working concentration and added on day 0 (Lats1/2 inhibitor Truli40 (CSNpharm, # CSN26140) or the Limk1 inhibitor Damnacanthal41 (Tocris, # 1936)) ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding was measured in the presence of 2 µM of Xanthine Amine Congener (XAC, Tocris Bioscience, Bristol, United Kingdom), and the resulting values were subtracted from those obtained with [3H]DPCPX35 to determine the specific binding and normalized for protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl) hydrazino]carbonyl]benzyl]benzene-sulfonamide (TCN 201, 3 μM dissolved in DMSO; Tocris Bioscience), (αR,βS)-α-(4-Hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidinepropanol maleate (Ro25-6981 ...
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bioRxiv - Neuroscience 2023Quote: ... The following compounds were added to raCSF as indicated in the Results section: D-(-)-2-Amino-5-phosphonopentanoic acid (D-APV, 100 μM dissolved in H2O; Tocris Bioscience), (5S,10R)-(+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate (MK-801 ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was then changed to 3N (50:50 DMEM/F12:neurobasal with N2 and B27 supplements) without vitamin A with 2 µM DMH1 (Tocris, 4126), 2 µM XAV939 (Cayman Chemical ...
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bioRxiv - Cell Biology 2022Quote: ... The FFA2R antagonist CATPB ((S)-3-(2-(3-chlorophenyl)acetamido)-4-(4-(trifluoromethyl)-phenyl) butanoic acid) was from Tocris (Britsol, UK).
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bioRxiv - Physiology 2023Quote: ... the cell medium was replaced with the experimental medium (2 ml/well) consisting of DMEM containing 1 nM of 1,25-dihydroxyvitamin D3 (Tocris; Cat. #2551), as described previously58 ...
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bioRxiv - Neuroscience 2023Quote: iPSCs were replated at a density of 2 x 104 cells/cm2 in mTeSR medium containing 10 µM Rho kinase inhibitor (Y-27632, Tocris #5849), which was removed after 24 hours ...