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Citations for Tocris :
1 - 50 of 68 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The craniotomy was then covered with 3% agar (MilliporeSigma) dissolved in aCSF (TOCRIS). The agar was prevented from drying out by covering it with silicone oil ...
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bioRxiv - Neuroscience 2023Quote: ... The craniotomy was then covered with 3% agar (MilliporeSigma) dissolved in aCSF (TOCRIS). The agar was covered with silicone oil (60,000 centistokes ...
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bioRxiv - Developmental Biology 2020Quote: ... and passaged 1:18 onto new Geltrex-coated 24-well plates for immunocytochemistry and 12-well plates for RNA preparation in mTeSR1 with 1uM thiazovivin (Tocris) (Day -1) ...
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bioRxiv - Cell Biology 2023Quote: ... 9,000 cells/well were seeded in a V-bottom ultra-low adhesion 96 well plate (#MS-9096VZ, Sbio prime surface 96V plate) in Essential 8 flex medium + Y-drug (#1254 Y-27632 dihydrochloride, Tocris), centrifuged at 3,000 rpm for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... and plate blocked with 300 µL 2% BSA (#5217, Tocris) in 1xPBS for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 hPSC-derived NMP-like cells were dissociated using Accutase and re-plated at a density of 37,500-86,000 cells /cm2 on Geltrex (Thermo Fisher)-coated plates directly in N2B27 containing CHIR99021 (3 µM, Tocris) and FGF2 (100 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... coated plates in E8 medium with 10 mM ROCK inhibitor (Tocris). After 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... coated plates in E8 with 10uM ROCK inhibitor (ROCKi; Tocris Y-27632) for transfection according to manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: AIM-100 plates were made by first dissolving AIM-100 (Tocris Cat. No. 4946) in a molecular grade 200 proof ethanol stock and then adding to NGM at a final concentration of 100 μM AIM-100 / 0.1% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were seeded in 6-well plates and treated with 25 μM AdipoRon (Tocris) or Adiponectin antibody (Cell Signaling Technology,1:200 ...
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bioRxiv - Biophysics 2021Quote: The parallel plate flow chamber was functionalized by incubating BSA (TOCRIS Bioscience, 9048-46-8), streptavidin (Cedarlane ...
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bioRxiv - Developmental Biology 2022Quote: ... and replated on Geltrex-coated plates in N2B27 with 10mM Y-27632 ROCK inhibitor (Tocris). The ROCK inhibitor was removed after 24 hours (day 8 ...
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bioRxiv - Bioengineering 2021Quote: ... Plates were centrifuged at 100xg for 3 minutes in E8 medium containing 10 μM Y27632 (Tocris). The following day ...
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bioRxiv - Microbiology 2022Quote: HEK293T cell suspension were seeded onto well plates in the presence of 50 μM CMK (Tocris). After 18 h upon confirming no signs of morphological change ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plated on Matrigel-coated plates at an appropriate density with ROCK inhibitor (1254, Tocris Bioscience, UK) at a final concentration of 10 μM for 24 hours to improve viability ...
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bioRxiv - Neuroscience 2024Quote: ... at seeding densities of 1000 cells/plate in hPSCs media containing 10 µm ROCK inhibitor (Y27632, ToCris). After individual colonies appear around day 14 ...
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bioRxiv - Microbiology 2024Quote: ... iPSC cells were passaged onto collagen-coated plates (2.4µg/mL) containing TeSR-E8 media with 10µM ROCK inhibitor Y-27632 (ROCKi; Tocris). Cells were then left to adhere to the plate for two hours while incubating at 37°C + 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was incubated for 20 min at 37°C and generic medium was added with ROCKi (Tocris) and Primocin for the first 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cell pellet was broken up and resuspended in 1ml E8 media by 1000ul pipette and distributed on a Geltrex coated 6 well plate with E8 and 10µM ROCK inhibitor (Y-27632 dihydrochloride, Tocris) for 24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and re-plated at 1:6 ratio on Matrigel-coated plate in neural medium supplemented with 1μM CHIR99021 (Tocris), 2μM DMH1 (Tocris) ...
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bioRxiv - Neuroscience 2020Quote: ... and re-plated at 1:6 ratio on Matrigel-coated plate in neural medium supplemented with 3μM CHIR99021 (Tocris), 2μM DMH1 (Tocris) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded at 7.5×105 cells per well of a 12-well plate on Matrigel two days before the differentiation medium (E6 medium, supplemented with either 10µM CHIR99021 (Tocris) for 2 days or with 3µM CHIR99021 and 0.5µM LDN193189 for six days) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were cultured on XF96e plates at 45,000/well density and treated with 1 µM Ara-C (Cytarabine, Tocris) for two days at 3 DIV as described above ...
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bioRxiv - Neuroscience 2022Quote: ... Matured iMGLs at density of 15 000 cells/well on 96-well plates were treated with 0.3-50 µM Yoda1 (Tocris) or equal volume of DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... of the cell suspension is reseeded to a new MEF containing 96-well plate pre-loaded with 100µL hPSC media containing 10 µM Rock inhibitor (Y27632, ToCris) and cultured for another 7 days with hPSC media.
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bioRxiv - Cell Biology 2024Quote: ... transgenic embryos were treated in 6-well plates at the indicated time points with 5 μM Ro5-3335 (Tocris) or DMSO (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Monolayers followed the same timing but were cultured in 6-well plates in 3.5 ml of medium and 5 µM XAV939 (Tocris) was included between day 3 and 6.
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were dissociated and 9,000 cells were seeded into each well of a U-shape 96-well plates in EB Formation Medium (Basal Medium 1 with Cerebral Organoid Supplement A) and 50 mM Y27632 (Tocris). The medium was changed every second day without Y27632 ...
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bioRxiv - Developmental Biology 2020Quote: ... confluent cells were digested by Accutase to single cells and seeded onto Geltrex-coated 12well plate at the dilution of 1:20 – 1:18 in mTeSR1 with 1uM Thiazovivin (Tocris). Next day ...
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Bioengineering 2020Quote: ... Biolamina)-coated 384-well plates in 30 μL of E8 media supplemented with CEPT cocktail or Y-27632 (10 μM, Tocris). After 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated single cells were then washed in PXGL or UXGL medium and plated onto MEF feeder plates with PXGL or UXGL medium supplemented with ROCK inhibitor 10 µM Y27632 (Tocris), and then re-fed with PXGL or UXGL medium every 2 days ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated and plated into pre-coated 6-well Aggrewell 800 plates at a concentration of 18M cells per well in hESC media with 10 µM Rock Inhibitor (ToCris). The next day (Day 1) ...
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bioRxiv - Neuroscience 2022Quote: Astrocytes grown in 6-well plates were refreshed with glial maintenance media or glial maintenance media supplemented with 100 μM L-Glutamic Acid (Tocris) for 3 h ...
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bioRxiv - Systems Biology 2023Quote: These stable cell lines were split into separate wells of a 24-well plate and either treated with doxycycline (Tocris) or left untreated until analyzed by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Microbiology 2024Quote: 5 × 104 BV2 cells were seeded in black walled 96-well plates and the following day media was removed and 100 nM NR12S (Tocris) diluted in DMEM without FBS was added to cells at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... A549 RIG-I−/− ACE2+ cells (80-90% confluent in 6-well plates) were transduced by adding 10 µg ml−1 Polybrene (Tocris) and recombinant lentivirus to a multiplicity of infection (MOI ...
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bioRxiv - Biochemistry 2024Quote: ... and plated on Geltrex coated 6 well plates at a density of 10,000 iPSCs/cm2 in 2ml/well of mTeSR1 + 10uM Y-27632 dihydrochloride (Tocris, 1254). Cells were cultured at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... PCTS and PCLS 8 mm in diameter were cultured in BioR plates in the Bioreactor and treated with sorafenib (10 µM – 20 µM) (Tocris), lenvatinib (0.25 µM – 1.0 µM ...
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bioRxiv - Cell Biology 2024Quote: ... coated 6-well plates with 1-1.5 million cells per well in E8 with 10 μM ROCK inhibitor Y-27632 (Tocris 1254). At day 1 of differentiation medium was exchanged to induction medium (IM ...
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bioRxiv - Biochemistry 2020Quote: ... and plated at 50,000 cells per plate in 2 mL of E8® media supplemented with 2 µL of 1000x Rho/Rock inhibitor Y27632 (Tocris). Prior to incubation with the appropriate peptide ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2022Quote: Macrophages were plated in a 12-well plate at 106 cells per well and treated with 10 μM E64 (Tocris, 5208) or 20 μM Z-Phe-Ala-FMK cathepsin B inhibitor (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... and singularized cells were seeded at a defined density (between 42,000 and 62,000 cells/cm2 depending on the hiPSC line) on matrigel coated 6-well plates in StemMACS iPS-Brew supplemented with 10 µM Y27632 (ROCK inhibitor, Tocris Bioscience). After 24 h (Fig ...
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bioRxiv - Genetics 2021Quote: ... 4-6 x 104 cells per well were seeded onto V-bottom 96 well plate in NPC differentiation media suppled with 10 µM Y27632 (Tocris; 1254) to generate embryonic bodies (EBs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...