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Citations for Tocris :
451 - 493 of 493 citations for Monocyclohexyl Phthalate Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Any unbound streptavidin-binding sites were blocked by a 1 min dip into 10 mg/mL biocytin (Tocris). Each set of biosensors was incubated with 25 nM solutions of IgG2 mAb139 in 1 x phosphate buffered saline in 6 min association/disassociation cycles ...
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bioRxiv - Genomics 2019Quote: ... 20ng/ml human LIF (all from WT-MRC Cambridge Stem Cell Institute) and 2 μM Gö6983 (PKCi; Tocris) on Matrigel–coated plates (Corning) ...
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bioRxiv - Neuroscience 2022Quote: ... 3,5-dichloro-N-[[(1α,5α,6-exo,6α)-3-(3,3-dimethylbutyl)-3-azabicyclo[3.1.0]hex-6-yl]methyl]-benzamide-hydrochloride (ML218 or ml) was purchased from TOCRIS and initially dissolved at 50 mM in DMSO ...
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bioRxiv - Molecular Biology 2022Quote: Parental A549 cells were fixed with 4% paraformaldehyde following 3-hour treatment with poly I:C (2.0 μg/mL, Tocris), LPS (1.0 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... EDL muscles were digested in 2.6 mg/mL collagenase in DMEM with Y-27632 (50 μM; Tocris Bioscience) for 55 min in 37℃ shaking water bath ...
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bioRxiv - Molecular Biology 2024Quote: The prepared crude synaptosome sample was diluted in PBS and stained with SynaptoRed C2 (1μg/ml, Tocris Bioscience) to label all plasma membrane-containing particles to increase synaptosome detection sensitivity ...
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bioRxiv - Neuroscience 2023Quote: ... Evoked AMPAR responses were taken from SPNs at baseline and followed by a bath-application of the CP-AMPAR antagonist 1-naphthyl acetylspermine (NASPM; Tocris 2756, 200 μM in 1:100 DMSO). Cells were incubated in NASPM for 10 mins before taking evoked AMPAR responses ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mL fresh RPMI 1640 containing B-27 supplement minus insulin was added to 0.5 mL conditioned (old) medium and added to cells along with 5 µM IWP2 (Tocris). Medium was aspirated at Day 5 and replenished with fresh RPMI 1640 containing B-27 supplement minus insulin ...
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bioRxiv - Neuroscience 2019Quote: ... To prevent eye movement during recordings α-bungarotoxin (1 nl of 2 mg/ml; Tocris, Bristol, UK; Cat: 2133) was injected into the ocular muscles behind the eye.
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bioRxiv - Neuroscience 2020Quote: ... Neurotoxic lesions were made by injecting either a solution of 5mg/ml NMDA in phosphate buffer (Tocris; pH 7.4) or a solution of quinolinic acid (Tocris ...
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bioRxiv - Cell Biology 2022Quote: ... Neuroectoderm was induced for 6 days in CDM-BSA supplemented with 12ng/ml FGF2 and 10μM SB431542 (Activin/Nodal/TGFβ inhibitor; Tocris), as previously described34 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... 3M cells on the second day of bFGF treatment were plated onto the chips in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) and washed 3h after plating.
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated from the 100 mm dishes and 2.8-3M cells per well were seeded on the prepared 2-well dishes in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) media for 3h ...
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bioRxiv - Bioengineering 2023Quote: ... 10 nM gastrin, 50 ng/ml recombinant human EGF (R&D, 236-EG) and 500 nM A83-01 (Tocris). Organoids were passaged every 4 days by physical dissociation using fire-polished Pasteur pipettes.
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bioRxiv - Cell Biology 2024Quote: ... The following inhibitors were resuspended in DMSO and used at the final concentration as indicated: Actinomycin D (1.0 and 2.5 µg/ml; Tocris; 1229); Puromycin (10ug/ml ...
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bioRxiv - Neuroscience 2020Quote: ... animals were intraperitoneal (i.p.) injected (0.1 ml/ 10 g) 1 hour prior to baseline freezing assessment with norbinalthorphimine (norBNI, Tocris Cat.# 0347) diluted in sterile saline (1 mg/ ml ...
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bioRxiv - Neuroscience 2021Quote: ... Hox propagation was initiated by activation of Wnt signaling using NMP media comprised of E6 medium supplemented with 200 ng/mL FGF8b and 3 μM CHIR99021 (Tocris). This constitutes the “Hox timepoint” of 0hrs ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were cultured for 36 hours in CDM-BSA (without insulin) supplemented with 20ng/ml FGF2 and 8µM CHIR99021 (WNT signalling activator; Tocris Bioscience). Subsequently cells were cultured for 4 days in CDM-BSA (with insulin ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Synthetic Biology 2020Quote: Cells expressing stably integrated or transiently transfected rTetR-effectors were assayed by flow cytometry during and after 5 days of 1 μg/mL doxycycline (Tocris) treatment ...
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bioRxiv - Developmental Biology 2020Quote: ... smFISH was then performed as above with the exception that 2.37 μg/ml Janelia Fluor 549 (Tocris, Cat. No. 6147) conjugated anti-GFP nanobody (Chromotek ...
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bioRxiv - Developmental Biology 2021Quote: ... fish were bathed in 200 ml fresh fish water containing 10 or 20 μM DMH1 diluted from a 10 mM DMH1 (Tocris) stock solution in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... The concentrations of hydrocortisone for both BEGM and ALI were increased to 0.48 μg/ml and BEGM was further supplemented with the inhibitors 1 μmol/L A83-01 (Tocris, USA), 3 μmol/L isoproterenol (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... These 30 µm slices were incubated with methoxy-X04 solution (0.004 mg/ml, Tocris Bioscience, 1:1 ethanol-PBS solution) at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia cells from C57Bl/6 J mice cultured in presence of CSF-1 were treated with Formoterol (10 ng/ml, cat # 1448, Tocris) or vehicle for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...
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bioRxiv - Cancer Biology 2022Quote: ... and plated at 60,000 cells/cm2 in N2B27 medium supplemented with 20 ng/ml of FGF2 (Cat# 233-FB/CF, R&D) and 4 μM of CHIR 99021 (Cat# 4423, Tocris) and 10 μM of Rock Inhibitor (Y-27632 ...
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bioRxiv - Biochemistry 2023Quote: ... Washed membranes were incubated in the hypotonic buffer in the presence of 2 mg/ml iodoacetamide and 50 μm SSR149415 (Tocris) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then rinsed with 3x with PBS before fixation with 4% paraformaldehyde in PBS with 10 μg/ml Hoechest 33342 (Tocris) for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... DIV12-13 neurons were pre-treated for 20 minutes with 40 ng/ml of the CXCR2 (CXCL1 receptor in neurons) antagonist SB225002 (Tocris Bioscience), prior to the addition of 40 nM recombinant mouse CXCL1 (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... and plated at 50,000 cells per plate in 2 mL of E8® media supplemented with 2 µL of 1000x Rho/Rock inhibitor Y27632 (Tocris). Prior to incubation with the appropriate peptide ...
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bioRxiv - Physiology 2023Quote: ... the cell medium was replaced with the experimental medium (2 ml/well) consisting of DMEM containing 1 nM of 1,25-dihydroxyvitamin D3 (Tocris; Cat. #2551), as described previously58 ...
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bioRxiv - Neuroscience 2023Quote: ... Allopregnanolone levels in the range of 0.2 to 200 ng/ml were measured against reference material purchased (Tocris Cat No. 3653) and a penta-deuterated stable isotope labeled internal standard (Tocris Cat No ...
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bioRxiv - Genetics 2023Quote: ... and 10 µg of the Lenti guide puro/blast-gRNA plasmid using 1 mg/ml of PEI transfection reagent (Tocris, 7854). Incubation with the transfection mix was done for 5 hours at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 1.0×105 cells/cm2 for anti-fibrotic condition consisting in FibroGRO 2% FBS supplemented with 75ng/mL of bFGF and 500nM of TGF-β inhibitor (A83-01, Tocris). iPSC-cFbs were maintained for 7 days in culture replacing medium each second day ...
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bioRxiv - Cell Biology 2019Quote: ... Primary cells were imaged in 100 µL DMEM/F12 containing 10% FCS and gentamycin (50 µg/mL) and were stimulated with either 150 µL PSS-Ca2+ or Yoda1 (20 µM, 5586, Tocris, prepared fresh) in PSS-Ca2+ (final concentration of Yoda1 is 12 µM) ...
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025], 250 nM Purmorphamine [Tocris, 4551] ...
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bioRxiv - Neuroscience 2024Quote: ... Cells in dissociation reagent were incubated for 15-20 min at 37οC to ensure dissociation of cell clusters before adding 5 mL of DMEM/F12 containing 0.04% BSA and 10% ROCK inhibitor Y-27632 (1/1000; Tocris; Cat. No. 1254), followed by resuspension by gentle pipetting ...
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bioRxiv - Developmental Biology 2020Quote: ... 9 month old Tg(id1-CRM2:GFP) fish were bathed in 300 ml fresh fish water containing 600 µl of a 10 mM DMH1 (Tocris, Wiesbaden-Nordenstadt, Germany) stock solution (final concentration 20 µM ...
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bioRxiv - Neuroscience 2020Quote: ... slices were equilibrated for 10 minutes while being continuously superfused at room temperature with oxygenated aCSF at 1-2 ml/min containing picrotoxin (50 μM, Tocris Bioscience, MO, USA) to block GABAA receptor-mediated inhibitory responses.
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bioRxiv - Developmental Biology 2022Quote: ... and 1% Pen/Strep) +2iLIF (10 ng/ml leukemia inhibitory factor + 3 μM CHIR99021 + 1 μM PD0325901 from Tocris, Cat. # 4423 and 4192, respectively). The cells were grown on 0.1% gelatin-coated dishes and usually seeded at a density of 0.5–1.5 × 104/cm2 ...