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Citations for Tocris :
351 - 400 of 421 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 3- (4,5-dimmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to estimate cell number following the manufacturer’s description (Tocris, # 5224), and absorbance was measured at 550 nm in an EnsightTM 96-well plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 1 uM of the microtubule-stabilizing agent SAHA (TOCRIS bioscience Cat. No. 149647-78-9) for 30 minutes before activation or experimental procedures ...
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bioRxiv - Genetics 2023Quote: ... cells underwent various drug experiments: for cAMP analogs (8Br-cAMP, db-cAMP, and 6Bnz-cAMP, Tocris Bioscience, Bristol, UK), they were incubated for 48 hours with the appropriate concentrations (50 and 500µM for 8Br-cAMP and db-cAMP ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in Anteriorization media-1 (100 ng/ml Noggin (R&D/Biotechne) and 10 μM SB431542 (Tocris)) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... mEPSC recordings were obtained from cells voltage-clamped at –70 mV in ACSF containing 0.5 µM tetrodotoxin (TTX, Tocris) and 50 µM picrotoxin (PTX ...
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bioRxiv - Neuroscience 2024Quote: Myelin degeneration was obtained by treating myelinated Schwann cell–DRG neuronal co-cultures with 25 μM AT–56 (Tocris) in myelinating medium for additional 7 days ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Neuroscience 2019Quote: ... The chemical inhibition of CTL6 cells was conducted by a global perfusion of clozapine-n-oxide (CNO, 5uM, Cat# 4936, Tocris), the chemical actuator of the chemogenetic probe ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Cell Biology 2021Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were either untreated (GFP-Apex and S1R-Apex) or treated (S1R-Apex) with 25μM Haloperidol (Tocris, Minneapolis, MN) or 20μM (+)-PTZ (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were rinsed with ice cold PBS and then collected and suspended by scraping in PBS containing 50nM JZL195 (Tocris), a dual inhibitor of FAAH and MAGL (Long et al. ...
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Immunology 2019Quote: ... To test the effect of VIP on ILC3, cells were incubated with different concentrations of VIP (human, rat, mouse, rabbit, canine, porcine) from Tocris, Bay 55-9837 (Tocris) ...
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 dissociated iPS cells resuspended in mTeSR1 media containing 10 µM Y-27362 dihydrochloride (ROCKi; Tocris Bioscience, cat. no. 1254) and 0.05% poly-vinyl alcohol (PVA ...
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bioRxiv - Genetics 2019Quote: ... cells were treated with phorbol 12-myristate 13-acetate (PMA; 100nM in DMSO) or DMSO or PKC antagonist (GF10930X; Tocris) for 24 hours following which cells were lysed for dual luciferase analysis as per manufacturer’s instructions (Promega UK).
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2022Quote: ... Then cells were hyperpolarized to -80 mV to isolate AMPA currents which were then were blocked by NBQX (20uM; Tocris) and finally the cells got depolarized to +40 mV to measure NMDA-mediated currents which were blocked by the selective NMDA antagonist AP-V (50 uM ...
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Cancer Biology 2023Quote: ... we washed cells once with PBS and then added various concentrations of drugs (tamoxifen, fulvenstrant, or 18albociclib; all purchased from Tocris) in phenol red free DMEM (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in an ad hoc defined generic medium (Supplementary Table 2) supplemented with Rho-kinase inhibitor (ROCKi; Tocris) and Primocin ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were continuously perfused with this saline solution during whole-cell recording with the addition of 20 μM NBQX and 20 μM (R)-CPP (Tocris) to isolate inhibitory synaptic activity ...
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bioRxiv - Developmental Biology 2023Quote: ... Mechanically dissociated cells in accutase were diluted with 4x volume of StemFlex with 10uM Y-27632 (ROCK inhibitor, Tocris, #1254) and manually counted with Trypan Blue ...
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bioRxiv - Systems Biology 2023Quote: These stable cell lines were split into separate wells of a 24-well plate and either treated with doxycycline (Tocris) or left untreated until analyzed by flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with quercetin for approximately 24 hours with DMSO used as a negative control and ActD (Tocris 1229) used as a positive control ...
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bioRxiv - Cancer Biology 2023Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase-dissociated single cells are FACS-sorted for the expression of the respective fluorescent marker protein and either directly used for bulk NGS based validation of the desired genome modification or subsequently plated at clonal density (250 to 350 cells/cm2) on MEFs in hESC media supplemented with 10 µm ROCK inhibitor (Y27632, ToCris) for the first 24 hr ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated and plated into pre-coated 6-well Aggrewell 800 plates at a concentration of 18M cells per well in hESC media with 10 µM Rock Inhibitor (ToCris). The next day (Day 1) ...
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bioRxiv - Immunology 2019Quote: ... Cell were then washed and rested overnight in complete RPMI medium without or with JAK kinase inhibitor CP-690550 (Tocris Biosciences), and stimulated for 4 hours with 100U/ml of IL-2 ...
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bioRxiv - Biochemistry 2020Quote: ... and plated at 50,000 cells per plate in 2 mL of E8® media supplemented with 2 µL of 1000x Rho/Rock inhibitor Y27632 (Tocris). Prior to incubation with the appropriate peptide ...
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bioRxiv - Neuroscience 2021Quote: ... cells were whole-cell voltage-clamped at −40mV and recorded in the presence of the GABAAR antagonist Gabazine (10 μM, Tocris 1262). N-methyl-D-aspartic acid (NMDA ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were seeded on Matrigel-coated plates or coverslips at a density of 1.1×105 cells/cm² in mTeSR1 medium supplemented with 10 μM of ROCK inhibitor Y-27632 (Ri; Tocris, ref. 1254). To induce MES differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2022Quote: Macrophages were plated in a 12-well plate at 106 cells per well and treated with 10 μM E64 (Tocris, 5208) or 20 μM Z-Phe-Ala-FMK cathepsin B inhibitor (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Human ePSCs were thawed at 37°C and plated on top of the feeder cells in EPSCM with 10 µM Y-27632 dihydrochloride (Tocris, 1254) for 48 h ...
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Bioengineering 2023Quote: ... and then seeded at a density of 3.8 × 10⁴ cells per well in 1% Geltrex-coated 24-well cell culture plates (30024, SPL Life Sciences) in mTeSR1 supplemented with Y-27632 dihydrochloride (10 μM) (1254, Tocris Bioscience). The medium was replaced with mTeSR1 the following day ...
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 µM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for ≤ 10 passages after thawing.
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bioRxiv - Cancer Biology 2023Quote: ... 1000 cells/well were seeded and pre-treated with SCFAs (SP or SB) for 48 hours followed by cisplatin (Tocris, 2251) treatment in a dose dependent manner for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting after treatment of the cells with 1 µM dTAG-7 (Tocris, 6912) for 3 h ...
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 μM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for no more than 10 passages after thawing ...